Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00825

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS00815 and BWI76_RS00820 are separated by 56 nucleotidesBWI76_RS00820 and BWI76_RS00825 are separated by 205 nucleotidesBWI76_RS00825 and BWI76_RS00830 are separated by 151 nucleotidesBWI76_RS00830 and BWI76_RS00835 are separated by 97 nucleotides BWI76_RS00815: BWI76_RS00815 - glycerol dehydrogenase, at 166,891 to 167,994 _RS00815 BWI76_RS00820: BWI76_RS00820 - fructose-6-phosphate aldolase, at 168,051 to 168,713 _RS00820 BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782 _RS00825 BWI76_RS00830: BWI76_RS00830 - metal-binding protein ZinT, at 169,934 to 170,581 _RS00830 BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330 _RS00835 Position (kb) 168 169 170Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 168.079 kb on + strandat 168.079 kb on + strandat 168.096 kb on + strandat 168.189 kb on + strand, within BWI76_RS00820at 168.297 kb on + strand, within BWI76_RS00820at 168.305 kb on - strand, within BWI76_RS00820at 168.384 kb on - strand, within BWI76_RS00820at 168.395 kb on + strand, within BWI76_RS00820at 168.481 kb on + strand, within BWI76_RS00820at 168.590 kb on - strand, within BWI76_RS00820at 168.742 kb on + strandat 168.848 kb on + strandat 168.978 kb on - strandat 169.099 kb on - strand, within BWI76_RS00825at 169.128 kb on + strand, within BWI76_RS00825at 169.137 kb on + strand, within BWI76_RS00825at 169.285 kb on + strand, within BWI76_RS00825at 169.293 kb on - strand, within BWI76_RS00825at 169.346 kb on - strand, within BWI76_RS00825at 169.495 kb on - strand, within BWI76_RS00825at 169.509 kb on + strand, within BWI76_RS00825at 169.529 kb on - strand, within BWI76_RS00825at 169.610 kb on + strand, within BWI76_RS00825at 169.625 kb on + strand, within BWI76_RS00825at 169.825 kb on + strandat 169.843 kb on - strandat 169.919 kb on + strandat 169.927 kb on - strandat 169.982 kb on - strandat 170.030 kb on - strand, within BWI76_RS00830at 170.082 kb on - strand, within BWI76_RS00830at 170.235 kb on - strand, within BWI76_RS00830at 170.241 kb on + strand, within BWI76_RS00830at 170.251 kb on + strand, within BWI76_RS00830at 170.552 kb on - strandat 170.664 kb on - strandat 170.694 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
168,079 + -1.3
168,079 + +1.2
168,096 + -1.0
168,189 + BWI76_RS00820 0.21 +2.2
168,297 + BWI76_RS00820 0.37 -0.5
168,305 - BWI76_RS00820 0.38 +1.0
168,384 - BWI76_RS00820 0.50 +0.5
168,395 + BWI76_RS00820 0.52 +0.4
168,481 + BWI76_RS00820 0.65 -1.3
168,590 - BWI76_RS00820 0.81 +0.5
168,742 + +0.6
168,848 + +2.1
168,978 - +1.3
169,099 - BWI76_RS00825 0.21 +0.2
169,128 + BWI76_RS00825 0.24 -0.4
169,137 + BWI76_RS00825 0.25 +0.8
169,285 + BWI76_RS00825 0.42 +1.0
169,293 - BWI76_RS00825 0.43 +1.0
169,346 - BWI76_RS00825 0.49 +1.2
169,495 - BWI76_RS00825 0.67 +0.1
169,509 + BWI76_RS00825 0.68 +1.6
169,529 - BWI76_RS00825 0.71 +2.3
169,610 + BWI76_RS00825 0.80 +3.0
169,625 + BWI76_RS00825 0.82 +1.3
169,825 + +1.3
169,843 - +0.9
169,919 + +0.9
169,927 - +2.8
169,982 - -0.3
170,030 - BWI76_RS00830 0.15 -1.6
170,082 - BWI76_RS00830 0.23 -0.0
170,235 - BWI76_RS00830 0.46 +0.0
170,241 + BWI76_RS00830 0.47 +0.7
170,251 + BWI76_RS00830 0.49 +0.7
170,552 - -4.2
170,664 - -0.5
170,694 - -0.6

Or see this region's nucleotide sequence