Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00060 and BWI76_RS00065 are separated by 147 nucleotides BWI76_RS00065 and BWI76_RS00070 are separated by 326 nucleotides
BWI76_RS00060: BWI76_RS00060 - N-acetylneuraminate lyase, at 11,227 to 12,123
_RS00060
BWI76_RS00065: BWI76_RS00065 - MurR/RpiR family transcriptional regulator, at 12,271 to 13,152
_RS00065
BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969
_RS00070
Position (kb)
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 11.485 kb on + strand, within BWI76_RS00060 at 11.485 kb on + strand, within BWI76_RS00060 at 11.697 kb on - strand, within BWI76_RS00060 at 11.935 kb on - strand, within BWI76_RS00060 at 12.356 kb on - strand at 12.428 kb on + strand, within BWI76_RS00065 at 12.436 kb on - strand, within BWI76_RS00065 at 12.465 kb on - strand, within BWI76_RS00065 at 12.527 kb on - strand, within BWI76_RS00065 at 12.584 kb on + strand, within BWI76_RS00065 at 12.584 kb on + strand, within BWI76_RS00065 at 12.695 kb on - strand, within BWI76_RS00065 at 12.749 kb on + strand, within BWI76_RS00065 at 12.749 kb on + strand, within BWI76_RS00065 at 12.822 kb on + strand, within BWI76_RS00065 at 13.124 kb on + strand at 13.253 kb on + strand at 13.471 kb on - strand at 13.471 kb on - strand at 13.575 kb on + strand at 13.624 kb on + strand at 13.648 kb on - strand, within BWI76_RS00070 at 13.693 kb on + strand, within BWI76_RS00070 at 13.794 kb on - strand, within BWI76_RS00070 at 13.842 kb on - strand, within BWI76_RS00070 at 13.858 kb on + strand, within BWI76_RS00070 at 13.858 kb on + strand, within BWI76_RS00070 at 13.980 kb on - strand, within BWI76_RS00070 at 13.993 kb on - strand, within BWI76_RS00070 at 14.009 kb on - strand, within BWI76_RS00070
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 11,485 + BWI76_RS00060 0.29 -1.8 11,485 + BWI76_RS00060 0.29 -1.2 11,697 - BWI76_RS00060 0.52 +1.2 11,935 - BWI76_RS00060 0.79 +0.3 12,356 - -1.0 12,428 + BWI76_RS00065 0.18 -1.3 12,436 - BWI76_RS00065 0.19 +1.4 12,465 - BWI76_RS00065 0.22 +2.8 12,527 - BWI76_RS00065 0.29 +1.1 12,584 + BWI76_RS00065 0.35 +0.0 12,584 + BWI76_RS00065 0.35 +1.2 12,695 - BWI76_RS00065 0.48 +2.3 12,749 + BWI76_RS00065 0.54 -3.3 12,749 + BWI76_RS00065 0.54 +0.6 12,822 + BWI76_RS00065 0.62 -1.1 13,124 + +0.6 13,253 + +0.5 13,471 - -3.2 13,471 - -0.8 13,575 + -0.9 13,624 + +0.2 13,648 - BWI76_RS00070 0.11 -1.5 13,693 + BWI76_RS00070 0.14 +1.3 13,794 - BWI76_RS00070 0.21 +0.8 13,842 - BWI76_RS00070 0.24 +0.6 13,858 + BWI76_RS00070 0.25 +1.0 13,858 + BWI76_RS00070 0.25 +1.3 13,980 - BWI76_RS00070 0.34 -0.1 13,993 - BWI76_RS00070 0.34 -1.8 14,009 - BWI76_RS00070 0.36 +1.0
Or see this region's nucleotide sequence