Experiment: RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25100 and BWI76_RS25105 are separated by 206 nucleotides BWI76_RS25105 and BWI76_RS25110 are separated by 130 nucleotides BWI76_RS25110 and BWI76_RS25115 overlap by 21 nucleotides BWI76_RS25115 and BWI76_RS25120 are separated by 153 nucleotides BWI76_RS25120 and BWI76_RS25125 are separated by 14 nucleotides BWI76_RS25125 and BWI76_RS25130 are separated by 229 nucleotides
BWI76_RS25100: BWI76_RS25100 - ribosome maturation factor, at 5,226,910 to 5,227,362
_RS25100
BWI76_RS25105: BWI76_RS25105 - tRNA-Met, at 5,227,569 to 5,227,645
_RS25105
BWI76_RS25110: BWI76_RS25110 - hypothetical protein, at 5,227,776 to 5,227,973
_RS25110
BWI76_RS25115: BWI76_RS25115 - argininosuccinate synthase, at 5,227,953 to 5,229,296
_RS25115
BWI76_RS25120: BWI76_RS25120 - tRNA-Leu, at 5,229,450 to 5,229,536
_RS25120
BWI76_RS25125: BWI76_RS25125 - preprotein translocase subunit SecG, at 5,229,551 to 5,229,880
_RS25125
BWI76_RS25130: BWI76_RS25130 - phosphoglucosamine mutase, at 5,230,110 to 5,231,447
_RS25130
Position (kb)
5227
5228
5229
5230 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 5228.036 kb on + strand at 5228.036 kb on + strand at 5228.054 kb on - strand at 5228.077 kb on + strand at 5228.228 kb on - strand, within BWI76_RS25115 at 5228.249 kb on + strand, within BWI76_RS25115 at 5228.359 kb on + strand, within BWI76_RS25115 at 5228.390 kb on + strand, within BWI76_RS25115 at 5228.455 kb on + strand, within BWI76_RS25115 at 5228.585 kb on - strand, within BWI76_RS25115 at 5228.994 kb on + strand, within BWI76_RS25115 at 5229.002 kb on - strand, within BWI76_RS25115 at 5229.007 kb on - strand, within BWI76_RS25115 at 5229.103 kb on - strand, within BWI76_RS25115 at 5230.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x remove 5,228,036 + -4.1 5,228,036 + -3.9 5,228,054 - -2.4 5,228,077 + -5.3 5,228,228 - BWI76_RS25115 0.20 -3.2 5,228,249 + BWI76_RS25115 0.22 -1.7 5,228,359 + BWI76_RS25115 0.30 -2.8 5,228,390 + BWI76_RS25115 0.33 -2.2 5,228,455 + BWI76_RS25115 0.37 -4.2 5,228,585 - BWI76_RS25115 0.47 -5.6 5,228,994 + BWI76_RS25115 0.77 -4.1 5,229,002 - BWI76_RS25115 0.78 -5.8 5,229,007 - BWI76_RS25115 0.78 -5.7 5,229,103 - BWI76_RS25115 0.86 -0.9 5,230,108 + +1.3
Or see this region's nucleotide sequence