Experiment: RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23255 and BWI76_RS23260 overlap by 10 nucleotides BWI76_RS23260 and BWI76_RS23265 are separated by 218 nucleotides BWI76_RS23265 and BWI76_RS23270 are separated by 227 nucleotides
BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503
_RS23255
BWI76_RS23260: BWI76_RS23260 - prepilin-type cleavage/methylation domain-containing protein, at 4,851,494 to 4,851,961
_RS23260
BWI76_RS23265: BWI76_RS23265 - hypothetical protein, at 4,852,180 to 4,853,856
_RS23265
BWI76_RS23270: BWI76_RS23270 - phosphoenolpyruvate--protein phosphotransferase PtsP, at 4,854,084 to 4,856,330
_RS23270
Position (kb) 4852
4853
4854 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.397 kb on - strand, within BWI76_RS23255 at 4851.399 kb on + strand, within BWI76_RS23255 at 4851.499 kb on + strand at 4851.617 kb on - strand, within BWI76_RS23260 at 4851.617 kb on - strand at 4851.642 kb on - strand, within BWI76_RS23260 at 4851.803 kb on + strand, within BWI76_RS23260 at 4851.945 kb on - strand at 4852.040 kb on - strand at 4853.023 kb on - strand, within BWI76_RS23265 at 4853.023 kb on - strand, within BWI76_RS23265 at 4854.079 kb on - strand at 4854.670 kb on + strand, within BWI76_RS23270 at 4854.740 kb on + strand, within BWI76_RS23270
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x remove 4,851,397 - BWI76_RS23255 0.81 +0.3 4,851,397 - BWI76_RS23255 0.81 +0.6 4,851,399 + BWI76_RS23255 0.81 -0.1 4,851,499 + +0.9 4,851,617 - BWI76_RS23260 0.26 +0.4 4,851,617 - +0.1 4,851,642 - BWI76_RS23260 0.32 -1.1 4,851,803 + BWI76_RS23260 0.66 +0.4 4,851,945 - -0.6 4,852,040 - +1.1 4,853,023 - BWI76_RS23265 0.50 -4.4 4,853,023 - BWI76_RS23265 0.50 -3.0 4,854,079 - +1.0 4,854,670 + BWI76_RS23270 0.26 -0.1 4,854,740 + BWI76_RS23270 0.29 +0.5
Or see this region's nucleotide sequence