Experiment: RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20065 and BWI76_RS20070 are separated by 14 nucleotides BWI76_RS20070 and BWI76_RS20075 overlap by 4 nucleotides BWI76_RS20075 and BWI76_RS20080 are separated by 77 nucleotides
BWI76_RS20065: BWI76_RS20065 - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,203,569 to 4,204,426
_RS20065
BWI76_RS20070: BWI76_RS20070 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 4,204,441 to 4,205,199
_RS20070
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
BWI76_RS20080: BWI76_RS20080 - isochorismate synthase MenF, at 4,206,944 to 4,208,269
_RS20080
Position (kb) 4205
4206
4207 Strain fitness (log2 ratio) -1
0
1 at 4204.489 kb on + strand at 4204.777 kb on + strand, within BWI76_RS20070 at 4205.061 kb on + strand, within BWI76_RS20070 at 4205.182 kb on - strand at 4205.899 kb on - strand at 4205.899 kb on - strand, within BWI76_RS20075 at 4206.257 kb on - strand, within BWI76_RS20075 at 4206.268 kb on + strand, within BWI76_RS20075 at 4206.414 kb on + strand, within BWI76_RS20075 at 4206.612 kb on - strand, within BWI76_RS20075 at 4206.761 kb on - strand at 4206.830 kb on + strand at 4206.845 kb on + strand at 4206.904 kb on - strand at 4207.023 kb on - strand at 4207.563 kb on - strand, within BWI76_RS20080 at 4207.664 kb on + strand, within BWI76_RS20080 at 4207.671 kb on + strand, within BWI76_RS20080
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x remove 4,204,489 + +0.3 4,204,777 + BWI76_RS20070 0.44 +1.2 4,205,061 + BWI76_RS20070 0.82 +0.1 4,205,182 - +1.6 4,205,899 - -0.7 4,205,899 - BWI76_RS20075 0.42 +0.3 4,206,257 - BWI76_RS20075 0.63 +0.4 4,206,268 + BWI76_RS20075 0.64 +0.4 4,206,414 + BWI76_RS20075 0.73 +0.6 4,206,612 - BWI76_RS20075 0.85 +1.2 4,206,761 - -1.1 4,206,830 + -0.6 4,206,845 + -1.4 4,206,904 - +0.2 4,207,023 - +0.5 4,207,563 - BWI76_RS20080 0.47 -0.2 4,207,664 + BWI76_RS20080 0.54 -0.5 4,207,671 + BWI76_RS20080 0.55 +0.5
Or see this region's nucleotide sequence