Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00965

Experiment: RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotidesBWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033 _RS00960 BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287 _RS00965 BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233 _RS00970 Position (kb) 197 198Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 196.149 kb on - strand, within BWI76_RS00960at 196.149 kb on - strand, within BWI76_RS00960at 196.541 kb on - strand, within BWI76_RS00960at 196.597 kb on + strandat 196.605 kb on - strand, within BWI76_RS00960at 196.754 kb on + strand, within BWI76_RS00960at 196.869 kb on + strandat 196.933 kb on + strandat 196.941 kb on - strandat 196.973 kb on + strandat 196.981 kb on - strandat 197.236 kb on - strand, within BWI76_RS00965at 197.330 kb on - strandat 197.383 kb on + strandat 197.399 kb on - strand, within BWI76_RS00970at 197.484 kb on + strand, within BWI76_RS00970at 197.484 kb on + strand, within BWI76_RS00970at 197.540 kb on + strand, within BWI76_RS00970at 197.540 kb on + strand, within BWI76_RS00970at 197.686 kb on + strand, within BWI76_RS00970at 198.044 kb on + strand, within BWI76_RS00970at 198.112 kb on + strand, within BWI76_RS00970at 198.168 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x
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196,149 - BWI76_RS00960 0.46 -2.2
196,149 - BWI76_RS00960 0.46 -1.1
196,541 - BWI76_RS00960 0.70 -2.3
196,597 + +1.2
196,605 - BWI76_RS00960 0.74 -4.2
196,754 + BWI76_RS00960 0.83 -0.1
196,869 + +0.4
196,933 + -0.1
196,941 - -0.9
196,973 + +0.1
196,981 - -2.0
197,236 - BWI76_RS00965 0.80 -2.4
197,330 - -0.8
197,383 + -1.1
197,399 - BWI76_RS00970 0.10 -2.5
197,484 + BWI76_RS00970 0.19 -1.0
197,484 + BWI76_RS00970 0.19 +1.2
197,540 + BWI76_RS00970 0.25 -2.7
197,540 + BWI76_RS00970 0.25 -0.1
197,686 + BWI76_RS00970 0.41 -1.6
198,044 + BWI76_RS00970 0.80 +1.2
198,112 + BWI76_RS00970 0.87 -0.2
198,168 + +1.5

Or see this region's nucleotide sequence