Experiment: RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotides BWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides BWI76_RS00855 and BWI76_RS00860 are separated by 31 nucleotides BWI76_RS00860 and BWI76_RS00865 overlap by 1 nucleotides
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708
_RS00850
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
BWI76_RS00860: BWI76_RS00860 - hypothetical protein, at 178,191 to 178,430
_RS00860
BWI76_RS00865: BWI76_RS00865 - DNA-binding transcriptional regulator OxyR, at 178,430 to 179,347
_RS00865
Position (kb) 176
177
178
179 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 175.803 kb on + strand, within BWI76_RS00845 at 175.825 kb on - strand at 176.129 kb on + strand, within BWI76_RS00850 at 176.171 kb on + strand, within BWI76_RS00850 at 176.235 kb on - strand, within BWI76_RS00850 at 176.263 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.358 kb on - strand, within BWI76_RS00850 at 176.583 kb on - strand, within BWI76_RS00850 at 176.612 kb on - strand, within BWI76_RS00850 at 176.652 kb on + strand at 176.655 kb on - strand at 176.669 kb on + strand at 176.669 kb on + strand at 176.737 kb on + strand at 176.861 kb on + strand at 176.876 kb on + strand at 176.917 kb on + strand at 176.966 kb on + strand, within BWI76_RS00855 at 176.994 kb on + strand, within BWI76_RS00855 at 177.129 kb on - strand, within BWI76_RS00855 at 177.132 kb on + strand, within BWI76_RS00855 at 177.164 kb on + strand, within BWI76_RS00855 at 177.231 kb on + strand, within BWI76_RS00855 at 177.375 kb on + strand, within BWI76_RS00855 at 177.458 kb on + strand, within BWI76_RS00855 at 177.466 kb on - strand, within BWI76_RS00855 at 177.598 kb on + strand, within BWI76_RS00855 at 177.712 kb on + strand, within BWI76_RS00855 at 177.719 kb on - strand, within BWI76_RS00855 at 177.744 kb on - strand, within BWI76_RS00855 at 178.145 kb on - strand at 178.161 kb on - strand at 178.161 kb on - strand at 178.175 kb on + strand at 178.551 kb on - strand, within BWI76_RS00865 at 178.618 kb on - strand, within BWI76_RS00865 at 178.761 kb on - strand, within BWI76_RS00865 at 178.943 kb on + strand, within BWI76_RS00865 at 179.153 kb on + strand, within BWI76_RS00865
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Glu_DLac_Acetate_mix 1 x remove 175,803 + BWI76_RS00845 0.88 -5.6 175,825 - -7.1 176,129 + BWI76_RS00850 0.25 -1.1 176,171 + BWI76_RS00850 0.30 -3.9 176,235 - BWI76_RS00850 0.39 -5.2 176,263 + BWI76_RS00850 0.42 -6.4 176,350 + BWI76_RS00850 0.54 -5.0 176,350 + BWI76_RS00850 0.54 -4.5 176,358 - BWI76_RS00850 0.55 -3.8 176,583 - BWI76_RS00850 0.84 -6.4 176,612 - BWI76_RS00850 0.87 -4.6 176,652 + -0.3 176,655 - -4.5 176,669 + -5.4 176,669 + -3.8 176,737 + -0.3 176,861 + -4.6 176,876 + -4.6 176,917 + -2.3 176,966 + BWI76_RS00855 0.13 -5.5 176,994 + BWI76_RS00855 0.15 -2.3 177,129 - BWI76_RS00855 0.25 -6.9 177,132 + BWI76_RS00855 0.25 -3.6 177,164 + BWI76_RS00855 0.28 -4.1 177,231 + BWI76_RS00855 0.32 -2.3 177,375 + BWI76_RS00855 0.43 -4.7 177,458 + BWI76_RS00855 0.49 -0.1 177,466 - BWI76_RS00855 0.49 -3.3 177,598 + BWI76_RS00855 0.59 -5.7 177,712 + BWI76_RS00855 0.67 +1.2 177,719 - BWI76_RS00855 0.68 -2.5 177,744 - BWI76_RS00855 0.70 -4.7 178,145 - -0.3 178,161 - -1.4 178,161 - +0.8 178,175 + +0.8 178,551 - BWI76_RS00865 0.13 +0.1 178,618 - BWI76_RS00865 0.20 +1.6 178,761 - BWI76_RS00865 0.36 -0.4 178,943 + BWI76_RS00865 0.56 -0.4 179,153 + BWI76_RS00865 0.79 -0.6
Or see this region's nucleotide sequence