Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24995 and BWI76_RS25000 are separated by 11 nucleotides BWI76_RS25000 and BWI76_RS25005 overlap by 8 nucleotides BWI76_RS25005 and BWI76_RS25010 overlap by 1 nucleotides
BWI76_RS24995: BWI76_RS24995 - malonate decarboxylase subunit alpha, at 5,205,172 to 5,206,839
_RS24995
BWI76_RS25000: BWI76_RS25000 - malonate decarboxylase acyl carrier protein, at 5,206,851 to 5,207,183
_RS25000
BWI76_RS25005: BWI76_RS25005 - biotin-independent malonate decarboxylase subunit beta, at 5,207,176 to 5,208,105
_RS25005
BWI76_RS25010: BWI76_RS25010 - malonyl-S-acyl carrier protein--biotin carboxyltransferase, at 5,208,105 to 5,208,986
_RS25010
Position (kb)
5206
5207
5208 Strain fitness (log2 ratio)
-2
-1
0
1 at 5206.068 kb on + strand, within BWI76_RS24995 at 5206.137 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.155 kb on - strand, within BWI76_RS24995 at 5206.192 kb on + strand, within BWI76_RS24995 at 5206.376 kb on - strand, within BWI76_RS24995 at 5206.641 kb on - strand, within BWI76_RS24995 at 5206.684 kb on + strand at 5206.806 kb on + strand at 5206.812 kb on - strand at 5206.854 kb on + strand at 5207.092 kb on - strand, within BWI76_RS25000 at 5207.131 kb on + strand, within BWI76_RS25000 at 5207.151 kb on - strand at 5207.296 kb on + strand, within BWI76_RS25005 at 5207.301 kb on + strand, within BWI76_RS25005 at 5207.339 kb on + strand, within BWI76_RS25005 at 5207.347 kb on - strand, within BWI76_RS25005 at 5207.549 kb on - strand, within BWI76_RS25005 at 5207.650 kb on - strand, within BWI76_RS25005 at 5208.002 kb on + strand, within BWI76_RS25005
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 5,206,068 + BWI76_RS24995 0.54 -0.1 5,206,137 + BWI76_RS24995 0.58 +1.5 5,206,152 + BWI76_RS24995 0.59 -1.9 5,206,152 + BWI76_RS24995 0.59 +0.1 5,206,155 - BWI76_RS24995 0.59 -0.1 5,206,192 + BWI76_RS24995 0.61 +0.3 5,206,376 - BWI76_RS24995 0.72 +0.3 5,206,641 - BWI76_RS24995 0.88 +0.1 5,206,684 + +0.4 5,206,806 + +0.1 5,206,812 - +0.3 5,206,854 + -0.5 5,207,092 - BWI76_RS25000 0.72 -2.3 5,207,131 + BWI76_RS25000 0.84 +1.1 5,207,151 - +0.3 5,207,296 + BWI76_RS25005 0.13 +0.1 5,207,301 + BWI76_RS25005 0.13 +0.5 5,207,339 + BWI76_RS25005 0.18 +0.0 5,207,347 - BWI76_RS25005 0.18 +0.8 5,207,549 - BWI76_RS25005 0.40 +0.6 5,207,650 - BWI76_RS25005 0.51 +0.6 5,208,002 + BWI76_RS25005 0.89 -2.0
Or see this region's nucleotide sequence