Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotides BWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides BWI76_RS21285 and BWI76_RS21290 are separated by 209 nucleotides
BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607
_RS21275
BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149
_RS21280
BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804
_RS21285
BWI76_RS21290: BWI76_RS21290 - DNA-binding transcriptional regulator, at 4,460,014 to 4,460,862
_RS21290
Position (kb) 4459
4460 Strain fitness (log2 ratio) -1
0
1
2 at 4458.374 kb on + strand, within BWI76_RS21275 at 4458.380 kb on + strand, within BWI76_RS21275 at 4458.382 kb on - strand, within BWI76_RS21275 at 4458.492 kb on - strand at 4458.607 kb on + strand at 4459.171 kb on + strand at 4459.398 kb on + strand, within BWI76_RS21285 at 4459.745 kb on - strand at 4459.988 kb on + strand at 4459.996 kb on - strand at 4460.011 kb on + strand at 4460.045 kb on + strand at 4460.053 kb on - strand at 4460.053 kb on - strand at 4460.256 kb on + strand, within BWI76_RS21290 at 4460.256 kb on + strand, within BWI76_RS21290 at 4460.333 kb on - strand, within BWI76_RS21290 at 4460.531 kb on + strand, within BWI76_RS21290 at 4460.579 kb on - strand, within BWI76_RS21290 at 4460.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,458,374 + BWI76_RS21275 0.86 +0.4 4,458,380 + BWI76_RS21275 0.86 +0.8 4,458,382 - BWI76_RS21275 0.86 +0.8 4,458,492 - +0.2 4,458,607 + +2.1 4,459,171 + +0.1 4,459,398 + BWI76_RS21285 0.36 +0.6 4,459,745 - +0.6 4,459,988 + +0.3 4,459,996 - -0.9 4,460,011 + +0.2 4,460,045 + -1.1 4,460,053 - +0.2 4,460,053 - +0.2 4,460,256 + BWI76_RS21290 0.29 -0.5 4,460,256 + BWI76_RS21290 0.29 +1.2 4,460,333 - BWI76_RS21290 0.38 -0.9 4,460,531 + BWI76_RS21290 0.61 -1.0 4,460,579 - BWI76_RS21290 0.67 -1.1 4,460,748 - +1.7
Or see this region's nucleotide sequence