Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16750 and BWI76_RS16755 are separated by 23 nucleotides BWI76_RS16755 and BWI76_RS16760 are separated by 57 nucleotides BWI76_RS16760 and BWI76_RS16765 overlap by 8 nucleotides
BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941
_RS16750
BWI76_RS16755: BWI76_RS16755 - acetoin reductase, at 3,470,965 to 3,471,735
_RS16755
BWI76_RS16760: BWI76_RS16760 - lipase, at 3,471,793 to 3,472,863
_RS16760
BWI76_RS16765: BWI76_RS16765 - spermidine/putrescine ABC transporter permease, at 3,472,856 to 3,474,625
_RS16765
Position (kb) 3471
3472
3473 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3470.925 kb on - strand at 3471.142 kb on - strand, within BWI76_RS16755 at 3471.229 kb on - strand, within BWI76_RS16755 at 3471.754 kb on - strand at 3471.859 kb on - strand at 3471.966 kb on - strand, within BWI76_RS16760 at 3471.970 kb on - strand, within BWI76_RS16760 at 3472.087 kb on - strand, within BWI76_RS16760 at 3472.494 kb on + strand at 3472.540 kb on - strand, within BWI76_RS16760 at 3472.540 kb on - strand, within BWI76_RS16760 at 3472.611 kb on + strand, within BWI76_RS16760 at 3472.998 kb on + strand at 3473.131 kb on + strand, within BWI76_RS16765 at 3473.269 kb on + strand, within BWI76_RS16765 at 3473.424 kb on - strand, within BWI76_RS16765
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,470,925 - +0.7 3,471,142 - BWI76_RS16755 0.23 +1.0 3,471,229 - BWI76_RS16755 0.34 +0.3 3,471,754 - +0.1 3,471,859 - -0.1 3,471,966 - BWI76_RS16760 0.16 +1.0 3,471,970 - BWI76_RS16760 0.17 -1.5 3,472,087 - BWI76_RS16760 0.27 +0.9 3,472,494 + -1.9 3,472,540 - BWI76_RS16760 0.70 +3.6 3,472,540 - BWI76_RS16760 0.70 +0.9 3,472,611 + BWI76_RS16760 0.76 +1.3 3,472,998 + +0.4 3,473,131 + BWI76_RS16765 0.16 +1.0 3,473,269 + BWI76_RS16765 0.23 +0.0 3,473,424 - BWI76_RS16765 0.32 -0.7
Or see this region's nucleotide sequence