Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16275

Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16270 and BWI76_RS16275 are separated by 142 nucleotidesBWI76_RS16275 and BWI76_RS16280 are separated by 197 nucleotides BWI76_RS16270: BWI76_RS16270 - fumarate hydratase, class II, at 3,375,140 to 3,376,540 _RS16270 BWI76_RS16275: BWI76_RS16275 - fumarate hydratase FumA, at 3,376,683 to 3,378,329 _RS16275 BWI76_RS16280: BWI76_RS16280 - mannose-6-phosphate isomerase, at 3,378,527 to 3,379,705 _RS16280 Position (kb) 3376 3377 3378 3379Strain fitness (log2 ratio) -3 -2 -1 0 1at 3375.706 kb on + strand, within BWI76_RS16270at 3376.030 kb on + strand, within BWI76_RS16270at 3376.146 kb on + strand, within BWI76_RS16270at 3376.166 kb on - strand, within BWI76_RS16270at 3376.270 kb on + strand, within BWI76_RS16270at 3376.367 kb on - strand, within BWI76_RS16270at 3376.473 kb on + strandat 3376.531 kb on + strandat 3376.909 kb on - strand, within BWI76_RS16275at 3377.840 kb on + strand, within BWI76_RS16275at 3378.245 kb on + strandat 3378.253 kb on + strandat 3378.256 kb on - strandat 3378.326 kb on + strandat 3378.546 kb on - strandat 3378.658 kb on - strand, within BWI76_RS16280at 3378.812 kb on + strand, within BWI76_RS16280at 3378.916 kb on + strand, within BWI76_RS16280

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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3,375,706 + BWI76_RS16270 0.40 +0.6
3,376,030 + BWI76_RS16270 0.64 -0.3
3,376,146 + BWI76_RS16270 0.72 +0.3
3,376,166 - BWI76_RS16270 0.73 +0.2
3,376,270 + BWI76_RS16270 0.81 +0.2
3,376,367 - BWI76_RS16270 0.88 +0.1
3,376,473 + +0.9
3,376,531 + -0.7
3,376,909 - BWI76_RS16275 0.14 -1.4
3,377,840 + BWI76_RS16275 0.70 -1.3
3,378,245 + -3.1
3,378,253 + -1.3
3,378,256 - -0.9
3,378,326 + +0.9
3,378,546 - +0.6
3,378,658 - BWI76_RS16280 0.11 -1.0
3,378,812 + BWI76_RS16280 0.24 -1.0
3,378,916 + BWI76_RS16280 0.33 +0.3

Or see this region's nucleotide sequence