Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16115 and BWI76_RS16120 are separated by 83 nucleotides BWI76_RS16120 and BWI76_RS16125 overlap by 11 nucleotides BWI76_RS16125 and BWI76_RS16130 overlap by 11 nucleotides
BWI76_RS16115: BWI76_RS16115 - fumarate hydratase, at 3,343,519 to 3,345,174
_RS16115
BWI76_RS16120: BWI76_RS16120 - 4-carboxymuconolactone decarboxylase, at 3,345,258 to 3,345,641
_RS16120
BWI76_RS16125: BWI76_RS16125 - 3-oxoadipate enol-lactonase, at 3,345,631 to 3,346,398
_RS16125
BWI76_RS16130: BWI76_RS16130 - 3-carboxy-cis,cis-muconate cycloisomerase, at 3,346,388 to 3,347,740
_RS16130
Position (kb) 3345
3346 Strain fitness (log2 ratio) -1
0
1
2 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.316 kb on - strand, within BWI76_RS16115 at 3344.603 kb on + strand, within BWI76_RS16115 at 3344.637 kb on + strand, within BWI76_RS16115 at 3344.689 kb on - strand, within BWI76_RS16115 at 3344.746 kb on - strand, within BWI76_RS16115 at 3345.032 kb on + strand at 3345.032 kb on + strand at 3345.228 kb on + strand at 3345.392 kb on - strand, within BWI76_RS16120 at 3345.440 kb on + strand, within BWI76_RS16120 at 3345.585 kb on + strand, within BWI76_RS16120 at 3345.593 kb on - strand, within BWI76_RS16120 at 3345.654 kb on + strand at 3345.903 kb on + strand, within BWI76_RS16125 at 3346.488 kb on - strand at 3346.494 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,344,316 - BWI76_RS16115 0.48 +0.6 3,344,316 - BWI76_RS16115 0.48 +0.0 3,344,603 + BWI76_RS16115 0.65 +0.2 3,344,637 + BWI76_RS16115 0.68 +2.0 3,344,689 - BWI76_RS16115 0.71 +0.2 3,344,746 - BWI76_RS16115 0.74 +0.3 3,345,032 + -0.2 3,345,032 + -0.5 3,345,228 + +1.4 3,345,392 - BWI76_RS16120 0.35 -0.2 3,345,440 + BWI76_RS16120 0.47 +2.0 3,345,585 + BWI76_RS16120 0.85 +0.7 3,345,593 - BWI76_RS16120 0.87 +0.0 3,345,654 + +1.0 3,345,903 + BWI76_RS16125 0.35 -0.4 3,346,488 - -0.6 3,346,494 + -1.0
Or see this region's nucleotide sequence