Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11640 and BWI76_RS11645 overlap by 28 nucleotides BWI76_RS11645 and BWI76_RS11650 are separated by 9 nucleotides BWI76_RS11650 and BWI76_RS11655 overlap by 4 nucleotides
BWI76_RS11640: BWI76_RS11640 - ABC transporter membrane protein, at 2,448,851 to 2,450,386
_RS11640
BWI76_RS11645: BWI76_RS11645 - ABC transporter substrate-binding protein, at 2,450,359 to 2,451,519
_RS11645
BWI76_RS11650: BWI76_RS11650 - carboxymuconolactone decarboxylase family protein, at 2,451,529 to 2,452,080
_RS11650
BWI76_RS11655: BWI76_RS11655 - TVP38/TMEM64 family protein, at 2,452,077 to 2,452,775
_RS11655
Position (kb)
2450
2451
2452 Strain fitness (log2 ratio)
-2
-1
0
1 at 2449.978 kb on + strand, within BWI76_RS11640 at 2449.986 kb on - strand, within BWI76_RS11640 at 2450.218 kb on - strand, within BWI76_RS11640 at 2450.364 kb on - strand at 2450.459 kb on - strand at 2450.526 kb on - strand, within BWI76_RS11645 at 2450.721 kb on + strand, within BWI76_RS11645 at 2450.729 kb on + strand, within BWI76_RS11645 at 2452.180 kb on - strand, within BWI76_RS11655 at 2452.253 kb on + strand, within BWI76_RS11655 at 2452.253 kb on + strand, within BWI76_RS11655 at 2452.303 kb on + strand, within BWI76_RS11655 at 2452.454 kb on + strand, within BWI76_RS11655 at 2452.477 kb on + strand, within BWI76_RS11655
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,449,978 + BWI76_RS11640 0.73 +0.4 2,449,986 - BWI76_RS11640 0.74 +0.1 2,450,218 - BWI76_RS11640 0.89 +0.5 2,450,364 - -1.6 2,450,459 - +0.7 2,450,526 - BWI76_RS11645 0.14 +0.9 2,450,721 + BWI76_RS11645 0.31 +0.5 2,450,729 + BWI76_RS11645 0.32 +0.5 2,452,180 - BWI76_RS11655 0.15 +0.8 2,452,253 + BWI76_RS11655 0.25 -0.1 2,452,253 + BWI76_RS11655 0.25 -0.5 2,452,303 + BWI76_RS11655 0.32 +0.2 2,452,454 + BWI76_RS11655 0.54 -2.5 2,452,477 + BWI76_RS11655 0.57 -0.1
Or see this region's nucleotide sequence