Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09780

Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09770 and BWI76_RS09775 overlap by 68 nucleotidesBWI76_RS09775 and BWI76_RS09780 are separated by 137 nucleotidesBWI76_RS09780 and BWI76_RS09785 are separated by 150 nucleotidesBWI76_RS09785 and BWI76_RS09790 are separated by 215 nucleotides BWI76_RS09765: BWI76_RS09765 - metalloprotease, at 2,048,747 to 2,049,514 _RS09765 BWI76_RS09770: BWI76_RS09770 - hypothetical protein, at 2,049,461 to 2,049,753 _RS09770 BWI76_RS09775: BWI76_RS09775 - cytidylate kinase, at 2,049,686 to 2,050,369 _RS09775 BWI76_RS09780: BWI76_RS09780 - 30S ribosomal protein S1, at 2,050,507 to 2,052,180 _RS09780 BWI76_RS09785: BWI76_RS09785 - integration host factor subunit beta, at 2,052,331 to 2,052,618 _RS09785 BWI76_RS09790: BWI76_RS09790 - ComEC family protein, at 2,052,834 to 2,055,089 _RS09790 Position (kb) 2050 2051 2052 2053Strain fitness (log2 ratio) -1 0 1at 2049.624 kb on - strand, within BWI76_RS09770at 2052.284 kb on + strandat 2052.704 kb on - strandat 2052.887 kb on - strandat 2052.918 kb on + strandat 2052.962 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,049,624 - BWI76_RS09770 0.56 -1.2
2,052,284 + -1.1
2,052,704 - +0.5
2,052,887 - +1.5
2,052,918 + +0.8
2,052,962 + -0.6

Or see this region's nucleotide sequence