Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04695 and BWI76_RS04700 are separated by 2 nucleotides BWI76_RS04700 and BWI76_RS04705 are separated by 180 nucleotides BWI76_RS04705 and BWI76_RS04710 are separated by 444 nucleotides BWI76_RS04710 and BWI76_RS04715 overlap by 2 nucleotides
BWI76_RS04695: BWI76_RS04695 - acetolactate synthase 3 large subunit, at 979,896 to 981,620
_RS04695
BWI76_RS04700: BWI76_RS04700 - acetolactate synthase small subunit, at 981,623 to 982,114
_RS04700
BWI76_RS04705: BWI76_RS04705 - DNA-binding transcriptional regulator FruR, at 982,295 to 983,299
_RS04705
BWI76_RS04710: BWI76_RS04710 - hypothetical protein, at 983,744 to 983,926
_RS04710
BWI76_RS04715: BWI76_RS04715 - division/cell wall cluster transcriptional repressor MraZ, at 983,925 to 984,383
_RS04715
Position (kb) 982
983
984 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 981.303 kb on + strand, within BWI76_RS04695 at 981.351 kb on + strand, within BWI76_RS04695 at 981.364 kb on - strand, within BWI76_RS04695 at 981.388 kb on - strand, within BWI76_RS04695 at 981.388 kb on - strand, within BWI76_RS04695 at 981.542 kb on - strand at 981.551 kb on + strand at 981.551 kb on + strand at 981.551 kb on + strand at 981.559 kb on - strand at 981.593 kb on - strand at 981.617 kb on - strand at 981.665 kb on + strand at 981.801 kb on + strand, within BWI76_RS04700 at 981.884 kb on - strand, within BWI76_RS04700 at 982.063 kb on + strand, within BWI76_RS04700 at 982.068 kb on + strand at 982.222 kb on + strand at 982.222 kb on + strand at 982.340 kb on + strand at 982.631 kb on + strand, within BWI76_RS04705 at 982.647 kb on - strand, within BWI76_RS04705 at 982.684 kb on - strand, within BWI76_RS04705 at 982.730 kb on + strand, within BWI76_RS04705 at 982.730 kb on + strand, within BWI76_RS04705 at 982.757 kb on + strand, within BWI76_RS04705 at 982.789 kb on - strand, within BWI76_RS04705 at 983.020 kb on + strand, within BWI76_RS04705 at 983.028 kb on - strand, within BWI76_RS04705 at 983.066 kb on - strand, within BWI76_RS04705 at 983.186 kb on - strand, within BWI76_RS04705 at 983.251 kb on - strand at 983.265 kb on + strand at 983.282 kb on + strand at 983.297 kb on - strand at 983.318 kb on - strand at 983.324 kb on + strand at 983.344 kb on - strand at 983.346 kb on - strand at 983.393 kb on - strand at 983.414 kb on - strand at 983.474 kb on + strand at 983.482 kb on - strand at 983.554 kb on + strand at 983.565 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.572 kb on + strand at 983.573 kb on - strand at 983.574 kb on + strand at 983.575 kb on + strand at 983.577 kb on + strand at 983.577 kb on + strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.580 kb on - strand at 983.582 kb on - strand at 983.619 kb on + strand at 983.626 kb on - strand at 983.627 kb on - strand at 983.636 kb on - strand at 983.637 kb on + strand at 983.638 kb on + strand at 983.670 kb on - strand at 983.710 kb on + strand at 983.718 kb on - strand at 983.824 kb on + strand, within BWI76_RS04710 at 983.826 kb on + strand, within BWI76_RS04710 at 983.834 kb on - strand, within BWI76_RS04710 at 983.849 kb on - strand, within BWI76_RS04710 at 983.922 kb on + strand at 984.062 kb on + strand, within BWI76_RS04715
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 981,303 + BWI76_RS04695 0.82 +0.1 981,351 + BWI76_RS04695 0.84 -0.2 981,364 - BWI76_RS04695 0.85 -0.9 981,388 - BWI76_RS04695 0.86 +0.3 981,388 - BWI76_RS04695 0.86 -1.7 981,542 - -0.2 981,551 + +0.0 981,551 + +0.7 981,551 + +0.5 981,559 - +0.7 981,593 - +0.6 981,617 - -0.9 981,665 + +1.5 981,801 + BWI76_RS04700 0.36 +0.7 981,884 - BWI76_RS04700 0.53 -0.1 982,063 + BWI76_RS04700 0.89 +0.0 982,068 + -0.2 982,222 + -0.2 982,222 + -0.8 982,340 + +1.1 982,631 + BWI76_RS04705 0.33 +0.2 982,647 - BWI76_RS04705 0.35 -1.6 982,684 - BWI76_RS04705 0.39 +0.5 982,730 + BWI76_RS04705 0.43 -1.9 982,730 + BWI76_RS04705 0.43 +0.1 982,757 + BWI76_RS04705 0.46 +0.8 982,789 - BWI76_RS04705 0.49 -1.9 983,020 + BWI76_RS04705 0.72 -2.1 983,028 - BWI76_RS04705 0.73 -1.8 983,066 - BWI76_RS04705 0.77 -0.5 983,186 - BWI76_RS04705 0.89 -0.5 983,251 - +1.6 983,265 + -1.1 983,282 + -0.9 983,297 - +0.1 983,318 - -0.9 983,324 + -0.8 983,344 - +0.1 983,346 - -0.3 983,393 - -1.8 983,414 - +0.5 983,474 + -0.8 983,482 - -0.7 983,554 + +0.5 983,565 + -0.2 983,572 + -0.5 983,572 + -1.8 983,572 + +0.1 983,572 + -0.7 983,572 + -0.3 983,572 + +0.0 983,573 - +0.1 983,574 + -0.9 983,575 + -0.2 983,577 + +0.1 983,577 + -0.2 983,580 - -0.3 983,580 - -1.5 983,580 - +0.1 983,580 - +0.2 983,580 - +0.6 983,580 - -0.5 983,580 - -0.3 983,580 - -0.3 983,580 - +0.3 983,580 - -0.3 983,580 - -0.6 983,580 - +1.3 983,580 - -1.2 983,582 - +1.4 983,619 + -0.2 983,626 - +0.5 983,627 - -0.6 983,636 - +0.7 983,637 + +0.3 983,638 + +0.5 983,670 - +0.1 983,710 + +0.1 983,718 - +1.1 983,824 + BWI76_RS04710 0.44 +1.1 983,826 + BWI76_RS04710 0.45 -3.0 983,834 - BWI76_RS04710 0.49 +0.8 983,849 - BWI76_RS04710 0.57 -1.1 983,922 + -0.9 984,062 + BWI76_RS04715 0.30 -2.0
Or see this region's nucleotide sequence