Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02985 and BWI76_RS02990 are separated by 112 nucleotides BWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotides BWI76_RS02995 and BWI76_RS03000 are separated by 36 nucleotides BWI76_RS03000 and BWI76_RS03005 are separated by 192 nucleotides BWI76_RS03005 and BWI76_RS03010 are separated by 190 nucleotides
BWI76_RS02985: BWI76_RS02985 - reactive intermediate/imine deaminase, at 613,013 to 613,399
_RS02985
BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973
_RS02990
BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921
_RS02995
BWI76_RS03000: BWI76_RS03000 - PyrBI operon leader peptide, at 614,958 to 615,062
_RS03000
BWI76_RS03005: BWI76_RS03005 - hypothetical protein, at 615,255 to 615,707
_RS03005
BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902
_RS03010
Position (kb) 613
614
615 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 613.314 kb on - strand, within BWI76_RS02985 at 613.348 kb on + strand, within BWI76_RS02985 at 613.349 kb on - strand, within BWI76_RS02985 at 613.552 kb on - strand at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.889 kb on + strand, within BWI76_RS02990 at 613.917 kb on + strand, within BWI76_RS02990 at 613.941 kb on - strand at 614.067 kb on + strand at 614.248 kb on - strand, within BWI76_RS02995 at 614.303 kb on - strand, within BWI76_RS02995 at 614.318 kb on + strand, within BWI76_RS02995 at 614.407 kb on - strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.469 kb on + strand, within BWI76_RS02995 at 614.477 kb on - strand, within BWI76_RS02995 at 614.488 kb on - strand, within BWI76_RS02995 at 614.503 kb on - strand, within BWI76_RS02995 at 614.557 kb on - strand, within BWI76_RS02995 at 614.919 kb on - strand at 615.106 kb on - strand at 615.140 kb on - strand at 615.140 kb on - strand at 615.279 kb on + strand at 615.300 kb on + strand at 615.323 kb on + strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.331 kb on - strand, within BWI76_RS03005 at 615.356 kb on + strand, within BWI76_RS03005 at 615.617 kb on + strand, within BWI76_RS03005 at 615.682 kb on - strand at 615.739 kb on - strand at 615.800 kb on - strand at 615.823 kb on + strand at 615.829 kb on - strand at 615.858 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 613,314 - BWI76_RS02985 0.78 -0.3 613,348 + BWI76_RS02985 0.87 -1.0 613,349 - BWI76_RS02985 0.87 +0.3 613,552 - -1.8 613,889 + BWI76_RS02990 0.82 +0.1 613,889 + BWI76_RS02990 0.82 -0.5 613,889 + BWI76_RS02990 0.82 +0.2 613,917 + BWI76_RS02990 0.88 -0.9 613,941 - -1.3 614,067 + -3.1 614,248 - BWI76_RS02995 0.28 -2.4 614,303 - BWI76_RS02995 0.34 -1.1 614,318 + BWI76_RS02995 0.35 -4.2 614,407 - BWI76_RS02995 0.45 -4.0 614,469 + BWI76_RS02995 0.52 -4.0 614,469 + BWI76_RS02995 0.52 -1.0 614,477 - BWI76_RS02995 0.52 -4.1 614,488 - BWI76_RS02995 0.54 -5.5 614,503 - BWI76_RS02995 0.55 +1.2 614,557 - BWI76_RS02995 0.61 -3.2 614,919 - -3.2 615,106 - -2.0 615,140 - +1.4 615,140 - +0.9 615,279 + -0.5 615,300 + +0.4 615,323 + BWI76_RS03005 0.15 +1.0 615,331 - BWI76_RS03005 0.17 +0.2 615,331 - BWI76_RS03005 0.17 -1.5 615,356 + BWI76_RS03005 0.22 -0.2 615,617 + BWI76_RS03005 0.80 -0.1 615,682 - +0.7 615,739 - -0.3 615,800 - -0.0 615,823 + +0.4 615,829 - -2.4 615,858 + +0.1
Or see this region's nucleotide sequence