Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01870

Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01865 and BWI76_RS01870 are separated by 129 nucleotidesBWI76_RS01870 and BWI76_RS01875 are separated by 122 nucleotidesBWI76_RS01875 and BWI76_RS01880 are separated by 69 nucleotides BWI76_RS01865: BWI76_RS01865 - glycerol-3-phosphate 1-O-acyltransferase, at 380,061 to 382,484 _RS01865 BWI76_RS01870: BWI76_RS01870 - diacylglycerol kinase, at 382,614 to 382,982 _RS01870 BWI76_RS01875: BWI76_RS01875 - repressor LexA, at 383,105 to 383,713 _RS01875 BWI76_RS01880: BWI76_RS01880 - MATE family efflux transporter, at 383,783 to 385,099 _RS01880 Position (kb) 382 383Strain fitness (log2 ratio) -3 -2 -1 0 1at 382.488 kb on - strandat 382.536 kb on - strandat 382.571 kb on + strandat 382.571 kb on + strandat 382.571 kb on + strandat 382.627 kb on + strandat 382.764 kb on - strand, within BWI76_RS01870at 382.798 kb on - strand, within BWI76_RS01870at 382.803 kb on - strand, within BWI76_RS01870at 382.873 kb on + strand, within BWI76_RS01870at 382.907 kb on - strand, within BWI76_RS01870at 382.995 kb on - strandat 383.810 kb on + strandat 383.868 kb on - strandat 383.899 kb on - strandat 383.927 kb on - strand, within BWI76_RS01880at 383.952 kb on + strand, within BWI76_RS01880

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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382,488 - -3.3
382,536 - -0.4
382,571 + -0.4
382,571 + +1.0
382,571 + +0.3
382,627 + -0.1
382,764 - BWI76_RS01870 0.41 -0.6
382,798 - BWI76_RS01870 0.50 -0.7
382,803 - BWI76_RS01870 0.51 -1.7
382,873 + BWI76_RS01870 0.70 -1.9
382,907 - BWI76_RS01870 0.79 -0.8
382,995 - +0.2
383,810 + +0.9
383,868 - +0.1
383,899 - +0.3
383,927 - BWI76_RS01880 0.11 +0.1
383,952 + BWI76_RS01880 0.13 +0.3

Or see this region's nucleotide sequence