Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00840 and BWI76_RS00845 are separated by 184 nucleotides BWI76_RS00845 and BWI76_RS00850 are separated by 9 nucleotides BWI76_RS00850 and BWI76_RS00855 are separated by 77 nucleotides
BWI76_RS00840: BWI76_RS00840 - acetylornithine deacetylase, at 173,585 to 174,736
_RS00840
BWI76_RS00845: BWI76_RS00845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 174,921 to 175,925
_RS00845
BWI76_RS00850: BWI76_RS00850 - acetylglutamate kinase, at 175,935 to 176,708
_RS00850
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
Position (kb) 174
175
176 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 173.960 kb on - strand, within BWI76_RS00840 at 173.960 kb on - strand, within BWI76_RS00840 at 174.257 kb on + strand, within BWI76_RS00840 at 174.352 kb on + strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.432 kb on - strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.445 kb on + strand, within BWI76_RS00840 at 174.453 kb on - strand, within BWI76_RS00840 at 174.497 kb on - strand, within BWI76_RS00840 at 174.674 kb on + strand at 174.707 kb on - strand at 174.966 kb on + strand at 174.996 kb on + strand at 175.207 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.291 kb on + strand, within BWI76_RS00845 at 175.329 kb on - strand, within BWI76_RS00845 at 175.483 kb on - strand, within BWI76_RS00845 at 175.498 kb on - strand, within BWI76_RS00845 at 175.593 kb on + strand, within BWI76_RS00845 at 175.709 kb on - strand, within BWI76_RS00845 at 175.714 kb on - strand, within BWI76_RS00845 at 175.739 kb on + strand, within BWI76_RS00845 at 175.775 kb on - strand, within BWI76_RS00845 at 175.803 kb on + strand, within BWI76_RS00845 at 175.825 kb on - strand at 176.129 kb on + strand, within BWI76_RS00850 at 176.171 kb on + strand, within BWI76_RS00850 at 176.235 kb on - strand, within BWI76_RS00850 at 176.263 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.350 kb on + strand, within BWI76_RS00850 at 176.358 kb on - strand, within BWI76_RS00850 at 176.583 kb on - strand, within BWI76_RS00850 at 176.612 kb on - strand, within BWI76_RS00850 at 176.652 kb on + strand at 176.655 kb on - strand at 176.669 kb on + strand at 176.669 kb on + strand at 176.737 kb on + strand at 176.861 kb on + strand at 176.876 kb on + strand at 176.917 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 173,960 - BWI76_RS00840 0.33 -0.4 173,960 - BWI76_RS00840 0.33 -0.2 174,257 + BWI76_RS00840 0.58 -0.2 174,352 + BWI76_RS00840 0.67 -0.1 174,432 - BWI76_RS00840 0.74 +0.4 174,432 - BWI76_RS00840 0.74 -0.4 174,445 + BWI76_RS00840 0.75 -0.4 174,445 + BWI76_RS00840 0.75 +0.1 174,453 - BWI76_RS00840 0.75 -0.0 174,497 - BWI76_RS00840 0.79 -0.2 174,674 + +0.4 174,707 - +0.5 174,966 + -3.0 174,996 + -1.1 175,207 + BWI76_RS00845 0.28 -4.2 175,291 + BWI76_RS00845 0.37 -6.3 175,291 + BWI76_RS00845 0.37 +1.2 175,329 - BWI76_RS00845 0.41 -2.6 175,483 - BWI76_RS00845 0.56 -3.4 175,498 - BWI76_RS00845 0.57 -2.0 175,593 + BWI76_RS00845 0.67 -4.3 175,709 - BWI76_RS00845 0.78 -1.8 175,714 - BWI76_RS00845 0.79 -4.7 175,739 + BWI76_RS00845 0.81 -1.8 175,775 - BWI76_RS00845 0.85 -2.0 175,803 + BWI76_RS00845 0.88 -4.6 175,825 - -5.1 176,129 + BWI76_RS00850 0.25 -3.6 176,171 + BWI76_RS00850 0.30 -3.9 176,235 - BWI76_RS00850 0.39 -4.1 176,263 + BWI76_RS00850 0.42 -3.5 176,350 + BWI76_RS00850 0.54 -4.0 176,350 + BWI76_RS00850 0.54 -5.1 176,358 - BWI76_RS00850 0.55 -3.7 176,583 - BWI76_RS00850 0.84 -5.7 176,612 - BWI76_RS00850 0.87 -3.0 176,652 + +0.1 176,655 - -4.5 176,669 + -5.4 176,669 + -4.1 176,737 + -0.1 176,861 + -4.5 176,876 + -4.9 176,917 + -2.2
Or see this region's nucleotide sequence