Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00825

Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00815 and BWI76_RS00820 are separated by 56 nucleotidesBWI76_RS00820 and BWI76_RS00825 are separated by 205 nucleotidesBWI76_RS00825 and BWI76_RS00830 are separated by 151 nucleotidesBWI76_RS00830 and BWI76_RS00835 are separated by 97 nucleotides BWI76_RS00815: BWI76_RS00815 - glycerol dehydrogenase, at 166,891 to 167,994 _RS00815 BWI76_RS00820: BWI76_RS00820 - fructose-6-phosphate aldolase, at 168,051 to 168,713 _RS00820 BWI76_RS00825: BWI76_RS00825 - AraC family transcriptional regulator, at 168,919 to 169,782 _RS00825 BWI76_RS00830: BWI76_RS00830 - metal-binding protein ZinT, at 169,934 to 170,581 _RS00830 BWI76_RS00835: BWI76_RS00835 - phosphoenolpyruvate carboxylase, at 170,679 to 173,330 _RS00835 Position (kb) 168 169 170Strain fitness (log2 ratio) -2 -1 0 1at 168.079 kb on + strandat 168.079 kb on + strandat 168.096 kb on + strandat 168.189 kb on + strand, within BWI76_RS00820at 168.297 kb on + strand, within BWI76_RS00820at 168.305 kb on - strand, within BWI76_RS00820at 168.384 kb on - strand, within BWI76_RS00820at 168.395 kb on + strand, within BWI76_RS00820at 168.481 kb on + strand, within BWI76_RS00820at 168.590 kb on - strand, within BWI76_RS00820at 168.742 kb on + strandat 168.848 kb on + strandat 168.978 kb on - strandat 169.099 kb on - strand, within BWI76_RS00825at 169.128 kb on + strand, within BWI76_RS00825at 169.137 kb on + strand, within BWI76_RS00825at 169.285 kb on + strand, within BWI76_RS00825at 169.293 kb on - strand, within BWI76_RS00825at 169.346 kb on - strand, within BWI76_RS00825at 169.495 kb on - strand, within BWI76_RS00825at 169.509 kb on + strand, within BWI76_RS00825at 169.529 kb on - strand, within BWI76_RS00825at 169.610 kb on + strand, within BWI76_RS00825at 169.625 kb on + strand, within BWI76_RS00825at 169.825 kb on + strandat 169.843 kb on - strandat 169.919 kb on + strandat 169.927 kb on - strandat 169.982 kb on - strandat 170.030 kb on - strand, within BWI76_RS00830at 170.082 kb on - strand, within BWI76_RS00830at 170.235 kb on - strand, within BWI76_RS00830at 170.241 kb on + strand, within BWI76_RS00830at 170.251 kb on + strand, within BWI76_RS00830at 170.552 kb on - strandat 170.664 kb on - strandat 170.694 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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168,079 + -1.1
168,079 + +0.3
168,096 + +0.2
168,189 + BWI76_RS00820 0.21 +1.3
168,297 + BWI76_RS00820 0.37 -0.6
168,305 - BWI76_RS00820 0.38 +0.6
168,384 - BWI76_RS00820 0.50 +0.4
168,395 + BWI76_RS00820 0.52 +0.0
168,481 + BWI76_RS00820 0.65 -1.1
168,590 - BWI76_RS00820 0.81 +0.3
168,742 + +0.6
168,848 + +0.0
168,978 - -1.4
169,099 - BWI76_RS00825 0.21 +0.2
169,128 + BWI76_RS00825 0.24 +0.3
169,137 + BWI76_RS00825 0.25 +0.4
169,285 + BWI76_RS00825 0.42 +0.5
169,293 - BWI76_RS00825 0.43 +1.2
169,346 - BWI76_RS00825 0.49 +0.7
169,495 - BWI76_RS00825 0.67 -0.1
169,509 + BWI76_RS00825 0.68 +0.9
169,529 - BWI76_RS00825 0.71 +1.2
169,610 + BWI76_RS00825 0.80 +0.2
169,625 + BWI76_RS00825 0.82 +1.2
169,825 + +0.1
169,843 - +0.7
169,919 + +0.6
169,927 - +1.2
169,982 - +0.4
170,030 - BWI76_RS00830 0.15 -1.4
170,082 - BWI76_RS00830 0.23 +0.5
170,235 - BWI76_RS00830 0.46 -0.8
170,241 + BWI76_RS00830 0.47 +0.3
170,251 + BWI76_RS00830 0.49 +0.3
170,552 - -0.7
170,664 - -2.3
170,694 - -0.4

Or see this region's nucleotide sequence