Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00740

Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00735 and BWI76_RS00740 are separated by 60 nucleotidesBWI76_RS00740 and BWI76_RS00745 are separated by 92 nucleotides BWI76_RS00735: BWI76_RS00735 - ATP-dependent 6-phosphofructokinase, at 149,499 to 150,461 _RS00735 BWI76_RS00740: BWI76_RS00740 - ribonuclease E activity regulator RraA, at 150,522 to 151,007 _RS00740 BWI76_RS00745: BWI76_RS00745 - 1,4-dihydroxy-2-naphthoate polyprenyltransferase, at 151,100 to 152,026 _RS00745 Position (kb) 150 151 152Strain fitness (log2 ratio) -1 0 1 2 3at 150.559 kb on - strandat 150.683 kb on - strand, within BWI76_RS00740at 150.827 kb on + strand, within BWI76_RS00740at 150.827 kb on - strand, within BWI76_RS00740at 150.867 kb on - strand, within BWI76_RS00740at 150.867 kb on - strand, within BWI76_RS00740at 150.963 kb on - strandat 151.169 kb on - strandat 151.176 kb on - strandat 151.316 kb on - strand, within BWI76_RS00745at 151.316 kb on - strand, within BWI76_RS00745at 151.443 kb on + strand, within BWI76_RS00745at 151.489 kb on + strand, within BWI76_RS00745at 151.489 kb on + strand, within BWI76_RS00745at 151.497 kb on - strand, within BWI76_RS00745at 151.497 kb on - strand, within BWI76_RS00745

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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150,559 - +2.8
150,683 - BWI76_RS00740 0.33 -0.5
150,827 + BWI76_RS00740 0.63 +0.9
150,827 - BWI76_RS00740 0.63 +0.4
150,867 - BWI76_RS00740 0.71 +0.6
150,867 - BWI76_RS00740 0.71 +0.9
150,963 - -0.3
151,169 - +0.2
151,176 - +1.2
151,316 - BWI76_RS00745 0.23 +0.2
151,316 - BWI76_RS00745 0.23 +0.1
151,443 + BWI76_RS00745 0.37 -0.8
151,489 + BWI76_RS00745 0.42 +0.2
151,489 + BWI76_RS00745 0.42 +0.1
151,497 - BWI76_RS00745 0.43 -0.8
151,497 - BWI76_RS00745 0.43 -0.8

Or see this region's nucleotide sequence