Experiment: RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00385 and BWI76_RS00390 are separated by 8 nucleotides BWI76_RS00390 and BWI76_RS00395 overlap by 49 nucleotides BWI76_RS00395 and BWI76_RS00400 are separated by 57 nucleotides
BWI76_RS00385: BWI76_RS00385 - nitrogen assimilation regulatory protein, at 80,878 to 82,287
_RS00385
BWI76_RS00390: BWI76_RS00390 - two-component system sensor histidine kinase NtrB, at 82,296 to 83,345
_RS00390
BWI76_RS00395: BWI76_RS00395 - hypothetical protein, at 83,297 to 83,476
_RS00395
BWI76_RS00400: BWI76_RS00400 - glutamine synthetase, at 83,534 to 84,943
_RS00400
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 81.432 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.600 kb on + strand, within BWI76_RS00385 at 81.652 kb on - strand, within BWI76_RS00385 at 81.934 kb on - strand, within BWI76_RS00385 at 82.010 kb on + strand, within BWI76_RS00385 at 82.010 kb on - strand, within BWI76_RS00385 at 82.201 kb on + strand at 82.201 kb on + strand at 82.209 kb on - strand at 82.209 kb on - strand at 82.255 kb on - strand at 82.334 kb on + strand at 82.334 kb on + strand at 82.400 kb on + strand at 82.493 kb on - strand, within BWI76_RS00390 at 82.861 kb on + strand, within BWI76_RS00390 at 82.889 kb on + strand, within BWI76_RS00390 at 83.033 kb on + strand, within BWI76_RS00390 at 83.040 kb on + strand, within BWI76_RS00390 at 83.142 kb on + strand, within BWI76_RS00390 at 83.186 kb on - strand, within BWI76_RS00390 at 83.302 kb on + strand at 83.501 kb on - strand at 83.508 kb on - strand at 83.541 kb on + strand at 83.636 kb on + strand at 83.695 kb on + strand, within BWI76_RS00400 at 83.819 kb on - strand, within BWI76_RS00400
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Arthrobacter sp. AnTP99 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 81,432 + BWI76_RS00385 0.39 -2.0 81,432 + BWI76_RS00385 0.39 -2.6 81,600 + BWI76_RS00385 0.51 -0.8 81,652 - BWI76_RS00385 0.55 -1.7 81,934 - BWI76_RS00385 0.75 -2.5 82,010 + BWI76_RS00385 0.80 -2.5 82,010 - BWI76_RS00385 0.80 -1.4 82,201 + -1.8 82,201 + +0.2 82,209 - -1.9 82,209 - -1.3 82,255 - -1.3 82,334 + -0.6 82,334 + -1.8 82,400 + -2.0 82,493 - BWI76_RS00390 0.19 -1.2 82,861 + BWI76_RS00390 0.54 -1.3 82,889 + BWI76_RS00390 0.56 -1.1 83,033 + BWI76_RS00390 0.70 -0.8 83,040 + BWI76_RS00390 0.71 -1.8 83,142 + BWI76_RS00390 0.81 -2.1 83,186 - BWI76_RS00390 0.85 -3.7 83,302 + -1.5 83,501 - -2.6 83,508 - -0.6 83,541 + -2.0 83,636 + +1.2 83,695 + BWI76_RS00400 0.11 +0.2 83,819 - BWI76_RS00400 0.20 +1.2
Or see this region's nucleotide sequence