Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24605

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24595 and BWI76_RS24600 are separated by 10 nucleotidesBWI76_RS24600 and BWI76_RS24605 are separated by 10 nucleotidesBWI76_RS24605 and BWI76_RS24610 are separated by 66 nucleotides BWI76_RS24595: BWI76_RS24595 - dihydroxyacetone kinase subunit DhaK, at 5,126,406 to 5,127,476 _RS24595 BWI76_RS24600: BWI76_RS24600 - dihydroxyacetone kinase, at 5,127,487 to 5,128,119 _RS24600 BWI76_RS24605: BWI76_RS24605 - dihydroxyacetone kinase subunit DhaM, at 5,128,130 to 5,129,551 _RS24605 BWI76_RS24610: BWI76_RS24610 - dihydroxyacetone kinase, at 5,129,618 to 5,131,267 _RS24610 Position (kb) 5128 5129 5130Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 5127.184 kb on - strand, within BWI76_RS24595at 5127.384 kb on - strandat 5127.401 kb on + strandat 5127.444 kb on + strandat 5127.444 kb on + strandat 5127.452 kb on - strandat 5127.456 kb on + strandat 5127.456 kb on + strandat 5127.464 kb on - strandat 5127.464 kb on - strandat 5127.526 kb on - strandat 5127.624 kb on + strand, within BWI76_RS24600at 5127.940 kb on - strand, within BWI76_RS24600at 5128.013 kb on - strand, within BWI76_RS24600at 5128.087 kb on + strandat 5128.266 kb on + strandat 5128.357 kb on - strand, within BWI76_RS24605at 5128.375 kb on + strand, within BWI76_RS24605at 5128.419 kb on - strand, within BWI76_RS24605at 5128.437 kb on - strand, within BWI76_RS24605at 5128.650 kb on + strand, within BWI76_RS24605at 5128.676 kb on + strand, within BWI76_RS24605at 5128.773 kb on + strand, within BWI76_RS24605at 5128.773 kb on + strand, within BWI76_RS24605at 5128.781 kb on - strand, within BWI76_RS24605at 5128.918 kb on + strand, within BWI76_RS24605at 5128.931 kb on - strand, within BWI76_RS24605at 5129.094 kb on - strand, within BWI76_RS24605at 5129.143 kb on - strand, within BWI76_RS24605at 5129.226 kb on + strand, within BWI76_RS24605at 5129.280 kb on + strand, within BWI76_RS24605at 5129.295 kb on + strand, within BWI76_RS24605at 5129.311 kb on + strand, within BWI76_RS24605at 5129.321 kb on + strand, within BWI76_RS24605at 5129.391 kb on - strand, within BWI76_RS24605at 5129.391 kb on - strand, within BWI76_RS24605at 5129.391 kb on - strand, within BWI76_RS24605at 5129.405 kb on - strand, within BWI76_RS24605at 5129.507 kb on + strandat 5129.577 kb on - strandat 5129.710 kb on + strandat 5130.313 kb on - strand, within BWI76_RS24610at 5130.313 kb on - strand, within BWI76_RS24610at 5130.313 kb on - strand, within BWI76_RS24610at 5130.313 kb on - strand, within BWI76_RS24610at 5130.346 kb on + strand, within BWI76_RS24610

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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5,127,184 - BWI76_RS24595 0.73 -1.2
5,127,384 - -0.5
5,127,401 + -0.5
5,127,444 + -1.2
5,127,444 + -0.1
5,127,452 - -1.5
5,127,456 + -1.3
5,127,456 + +0.6
5,127,464 - -0.4
5,127,464 - +1.1
5,127,526 - -0.9
5,127,624 + BWI76_RS24600 0.22 -0.0
5,127,940 - BWI76_RS24600 0.72 -0.5
5,128,013 - BWI76_RS24600 0.83 -2.4
5,128,087 + +0.2
5,128,266 + +0.1
5,128,357 - BWI76_RS24605 0.16 -0.9
5,128,375 + BWI76_RS24605 0.17 -1.5
5,128,419 - BWI76_RS24605 0.20 -0.9
5,128,437 - BWI76_RS24605 0.22 -2.7
5,128,650 + BWI76_RS24605 0.37 -0.5
5,128,676 + BWI76_RS24605 0.38 +1.1
5,128,773 + BWI76_RS24605 0.45 +1.1
5,128,773 + BWI76_RS24605 0.45 -4.1
5,128,781 - BWI76_RS24605 0.46 -0.5
5,128,918 + BWI76_RS24605 0.55 -1.2
5,128,931 - BWI76_RS24605 0.56 -1.1
5,129,094 - BWI76_RS24605 0.68 -0.9
5,129,143 - BWI76_RS24605 0.71 -2.2
5,129,226 + BWI76_RS24605 0.77 -0.9
5,129,280 + BWI76_RS24605 0.81 -1.9
5,129,295 + BWI76_RS24605 0.82 +1.1
5,129,311 + BWI76_RS24605 0.83 +0.8
5,129,321 + BWI76_RS24605 0.84 -1.4
5,129,391 - BWI76_RS24605 0.89 -2.0
5,129,391 - BWI76_RS24605 0.89 +0.1
5,129,391 - BWI76_RS24605 0.89 -0.5
5,129,405 - BWI76_RS24605 0.90 +1.1
5,129,507 + -2.4
5,129,577 - -0.9
5,129,710 + -0.9
5,130,313 - BWI76_RS24610 0.42 -2.7
5,130,313 - BWI76_RS24610 0.42 +1.1
5,130,313 - BWI76_RS24610 0.42 -2.0
5,130,313 - BWI76_RS24610 0.42 -0.9
5,130,346 + BWI76_RS24610 0.44 -0.2

Or see this region's nucleotide sequence