Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20780 and BWI76_RS20785 overlap by 11 nucleotides BWI76_RS20785 and BWI76_RS20790 are separated by 10 nucleotides BWI76_RS20790 and BWI76_RS20795 are separated by 11 nucleotides BWI76_RS20795 and BWI76_RS20800 are separated by 114 nucleotides BWI76_RS20800 and BWI76_RS20805 are separated by 36 nucleotides
BWI76_RS20780: BWI76_RS20780 - alcohol dehydrogenase EutG, at 4,343,873 to 4,345,060
_RS20780
BWI76_RS20785: BWI76_RS20785 - ethanolamine utilization protein EutJ, at 4,345,050 to 4,345,889
_RS20785
BWI76_RS20790: BWI76_RS20790 - aldehyde dehydrogenase, at 4,345,900 to 4,347,303
_RS20790
BWI76_RS20795: BWI76_RS20795 - ethanolamine catabolic microcompartment shell protein EutN, at 4,347,315 to 4,347,602
_RS20795
BWI76_RS20800: BWI76_RS20800 - carboxysome shell protein, at 4,347,717 to 4,348,007
_RS20800
BWI76_RS20805: BWI76_RS20805 - phosphate acetyltransferase, at 4,348,044 to 4,349,060
_RS20805
Position (kb) 4345
4346
4347
4348 Strain fitness (log2 ratio) -1
0
1
2 at 4344.993 kb on + strand at 4345.529 kb on + strand, within BWI76_RS20785 at 4345.579 kb on - strand at 4345.916 kb on + strand at 4346.010 kb on - strand at 4346.342 kb on + strand, within BWI76_RS20790 at 4346.544 kb on - strand, within BWI76_RS20790 at 4346.605 kb on + strand, within BWI76_RS20790 at 4346.644 kb on - strand, within BWI76_RS20790 at 4347.523 kb on + strand, within BWI76_RS20795 at 4347.955 kb on + strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800 at 4347.963 kb on - strand, within BWI76_RS20800
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,344,993 + -0.4 4,345,529 + BWI76_RS20785 0.57 -0.1 4,345,579 - -0.9 4,345,916 + +0.4 4,346,010 - +0.9 4,346,342 + BWI76_RS20790 0.31 +0.6 4,346,544 - BWI76_RS20790 0.46 +1.7 4,346,605 + BWI76_RS20790 0.50 +0.5 4,346,644 - BWI76_RS20790 0.53 +0.0 4,347,523 + BWI76_RS20795 0.72 +0.5 4,347,955 + BWI76_RS20800 0.82 +0.6 4,347,963 - BWI76_RS20800 0.85 +0.1 4,347,963 - BWI76_RS20800 0.85 +2.0
Or see this region's nucleotide sequence