Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18265

Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18260 and BWI76_RS18265 are separated by 252 nucleotidesBWI76_RS18265 and BWI76_RS18270 are separated by 46 nucleotidesBWI76_RS18270 and BWI76_RS18275 are separated by 371 nucleotides BWI76_RS18260: BWI76_RS18260 - ferritin, at 3,794,989 to 3,795,492 _RS18260 BWI76_RS18265: BWI76_RS18265 - MFS transporter, at 3,795,745 to 3,797,307 _RS18265 BWI76_RS18270: BWI76_RS18270 - hypothetical protein, at 3,797,354 to 3,797,605 _RS18270 BWI76_RS18275: BWI76_RS18275 - MFS transporter, at 3,797,977 to 3,799,401 _RS18275 Position (kb) 3795 3796 3797 3798Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3794.860 kb on + strandat 3794.860 kb on + strandat 3794.896 kb on - strandat 3794.933 kb on + strandat 3794.939 kb on - strandat 3794.940 kb on - strandat 3794.940 kb on - strandat 3794.942 kb on + strandat 3794.947 kb on + strandat 3794.947 kb on + strandat 3794.947 kb on + strandat 3794.954 kb on + strandat 3794.954 kb on + strandat 3794.954 kb on + strandat 3794.955 kb on + strandat 3794.955 kb on + strandat 3794.955 kb on - strandat 3794.963 kb on + strandat 3795.077 kb on + strand, within BWI76_RS18260at 3795.082 kb on + strand, within BWI76_RS18260at 3795.082 kb on + strand, within BWI76_RS18260at 3795.082 kb on + strand, within BWI76_RS18260at 3795.085 kb on - strand, within BWI76_RS18260at 3795.088 kb on - strand, within BWI76_RS18260at 3795.090 kb on - strandat 3795.090 kb on - strand, within BWI76_RS18260at 3795.090 kb on - strand, within BWI76_RS18260at 3795.090 kb on - strand, within BWI76_RS18260at 3795.090 kb on - strand, within BWI76_RS18260at 3795.090 kb on - strand, within BWI76_RS18260at 3795.091 kb on + strand, within BWI76_RS18260at 3795.099 kb on - strand, within BWI76_RS18260at 3795.132 kb on + strand, within BWI76_RS18260at 3795.137 kb on + strand, within BWI76_RS18260at 3795.145 kb on - strand, within BWI76_RS18260at 3795.145 kb on - strand, within BWI76_RS18260at 3795.235 kb on + strand, within BWI76_RS18260at 3795.289 kb on + strand, within BWI76_RS18260at 3795.289 kb on + strand, within BWI76_RS18260at 3795.337 kb on - strand, within BWI76_RS18260at 3795.479 kb on - strandat 3795.493 kb on + strandat 3795.500 kb on - strandat 3795.500 kb on - strandat 3795.549 kb on - strandat 3795.553 kb on - strandat 3795.558 kb on - strandat 3795.656 kb on + strandat 3795.664 kb on - strandat 3795.774 kb on - strandat 3795.778 kb on - strandat 3795.822 kb on - strandat 3795.899 kb on + strandat 3796.023 kb on - strand, within BWI76_RS18265at 3796.077 kb on + strand, within BWI76_RS18265at 3796.082 kb on + strand, within BWI76_RS18265at 3796.082 kb on + strand, within BWI76_RS18265at 3796.213 kb on - strand, within BWI76_RS18265at 3796.277 kb on + strand, within BWI76_RS18265at 3796.277 kb on + strand, within BWI76_RS18265at 3796.369 kb on - strand, within BWI76_RS18265at 3796.439 kb on + strand, within BWI76_RS18265at 3796.442 kb on - strand, within BWI76_RS18265at 3796.606 kb on + strand, within BWI76_RS18265at 3796.661 kb on - strand, within BWI76_RS18265at 3796.731 kb on + strand, within BWI76_RS18265at 3796.736 kb on + strand, within BWI76_RS18265at 3796.739 kb on - strand, within BWI76_RS18265at 3796.739 kb on - strand, within BWI76_RS18265at 3796.739 kb on - strand, within BWI76_RS18265at 3796.835 kb on + strand, within BWI76_RS18265at 3797.154 kb on + strandat 3797.431 kb on + strand, within BWI76_RS18270at 3797.761 kb on + strandat 3797.922 kb on + strandat 3797.922 kb on + strandat 3797.925 kb on - strandat 3797.925 kb on - strandat 3797.930 kb on - strandat 3797.949 kb on - strandat 3798.093 kb on - strandat 3798.299 kb on - strand, within BWI76_RS18275

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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3,794,860 + -0.6
3,794,860 + -0.2
3,794,896 - +0.4
3,794,933 + +0.0
3,794,939 - +0.4
3,794,940 - +1.0
3,794,940 - -0.3
3,794,942 + +0.5
3,794,947 + -0.2
3,794,947 + -1.1
3,794,947 + +0.4
3,794,954 + -1.1
3,794,954 + -0.8
3,794,954 + -1.1
3,794,955 + -0.1
3,794,955 + +0.9
3,794,955 - -0.9
3,794,963 + +1.1
3,795,077 + BWI76_RS18260 0.17 -0.6
3,795,082 + BWI76_RS18260 0.18 -2.0
3,795,082 + BWI76_RS18260 0.18 +3.3
3,795,082 + BWI76_RS18260 0.18 -0.1
3,795,085 - BWI76_RS18260 0.19 +0.3
3,795,088 - BWI76_RS18260 0.20 -1.1
3,795,090 - +0.9
3,795,090 - BWI76_RS18260 0.20 -0.6
3,795,090 - BWI76_RS18260 0.20 +0.8
3,795,090 - BWI76_RS18260 0.20 -0.1
3,795,090 - BWI76_RS18260 0.20 -0.2
3,795,090 - BWI76_RS18260 0.20 +1.1
3,795,091 + BWI76_RS18260 0.20 -1.9
3,795,099 - BWI76_RS18260 0.22 +0.9
3,795,132 + BWI76_RS18260 0.28 -1.6
3,795,137 + BWI76_RS18260 0.29 +1.9
3,795,145 - BWI76_RS18260 0.31 +0.5
3,795,145 - BWI76_RS18260 0.31 -1.1
3,795,235 + BWI76_RS18260 0.49 -1.2
3,795,289 + BWI76_RS18260 0.60 +0.2
3,795,289 + BWI76_RS18260 0.60 -0.3
3,795,337 - BWI76_RS18260 0.69 -0.7
3,795,479 - +0.5
3,795,493 + -0.5
3,795,500 - +1.4
3,795,500 - -0.9
3,795,549 - -0.0
3,795,553 - +0.2
3,795,558 - +0.7
3,795,656 + -0.2
3,795,664 - -0.6
3,795,774 - -0.4
3,795,778 - -0.4
3,795,822 - +1.8
3,795,899 + -0.2
3,796,023 - BWI76_RS18265 0.18 +3.3
3,796,077 + BWI76_RS18265 0.21 -0.1
3,796,082 + BWI76_RS18265 0.22 -0.7
3,796,082 + BWI76_RS18265 0.22 -1.0
3,796,213 - BWI76_RS18265 0.30 -0.1
3,796,277 + BWI76_RS18265 0.34 -0.9
3,796,277 + BWI76_RS18265 0.34 -0.2
3,796,369 - BWI76_RS18265 0.40 -0.1
3,796,439 + BWI76_RS18265 0.44 -1.6
3,796,442 - BWI76_RS18265 0.45 -0.4
3,796,606 + BWI76_RS18265 0.55 -0.1
3,796,661 - BWI76_RS18265 0.59 -2.2
3,796,731 + BWI76_RS18265 0.63 +0.7
3,796,736 + BWI76_RS18265 0.63 -2.6
3,796,739 - BWI76_RS18265 0.64 -2.5
3,796,739 - BWI76_RS18265 0.64 -1.1
3,796,739 - BWI76_RS18265 0.64 -2.4
3,796,835 + BWI76_RS18265 0.70 -0.0
3,797,154 + -1.1
3,797,431 + BWI76_RS18270 0.31 +0.2
3,797,761 + -0.4
3,797,922 + +0.6
3,797,922 + -1.4
3,797,925 - -0.1
3,797,925 - +0.1
3,797,930 - -1.6
3,797,949 - +0.6
3,798,093 - -0.4
3,798,299 - BWI76_RS18275 0.23 +0.5

Or see this region's nucleotide sequence