Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06975 and BWI76_RS06980 are separated by 210 nucleotides BWI76_RS06980 and BWI76_RS06985 are separated by 91 nucleotides BWI76_RS06985 and BWI76_RS06990 are separated by 88 nucleotides
BWI76_RS06975: BWI76_RS06975 - DNA-binding transcriptional activator AllS, at 1,465,061 to 1,465,984
_RS06975
BWI76_RS06980: BWI76_RS06980 - ureidoglycolate lyase, at 1,466,195 to 1,466,677
_RS06980
BWI76_RS06985: BWI76_RS06985 - transcriptional regulator, at 1,466,769 to 1,467,587
_RS06985
BWI76_RS06990: BWI76_RS06990 - glyoxylate carboligase, at 1,467,676 to 1,469,457
_RS06990
Position (kb) 1466
1467
1468 Strain fitness (log2 ratio) -1
0
1 at 1465.925 kb on + strand at 1465.928 kb on - strand at 1466.133 kb on + strand at 1466.152 kb on + strand at 1466.153 kb on - strand at 1466.499 kb on + strand, within BWI76_RS06980 at 1466.835 kb on + strand at 1466.978 kb on - strand, within BWI76_RS06985 at 1467.603 kb on + strand at 1467.852 kb on + strand at 1467.891 kb on + strand, within BWI76_RS06990 at 1467.933 kb on + strand, within BWI76_RS06990 at 1468.137 kb on + strand, within BWI76_RS06990 at 1468.161 kb on + strand, within BWI76_RS06990 at 1468.169 kb on - strand, within BWI76_RS06990 at 1468.530 kb on + strand, within BWI76_RS06990 at 1468.548 kb on + strand, within BWI76_RS06990
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,465,925 + +0.3 1,465,928 - +0.5 1,466,133 + -0.9 1,466,152 + -0.1 1,466,153 - +1.0 1,466,499 + BWI76_RS06980 0.63 +0.4 1,466,835 + -1.3 1,466,978 - BWI76_RS06985 0.26 +0.1 1,467,603 + -1.0 1,467,852 + +0.2 1,467,891 + BWI76_RS06990 0.12 +0.0 1,467,933 + BWI76_RS06990 0.14 -0.6 1,468,137 + BWI76_RS06990 0.26 -0.1 1,468,161 + BWI76_RS06990 0.27 +0.3 1,468,169 - BWI76_RS06990 0.28 +0.2 1,468,530 + BWI76_RS06990 0.48 +0.1 1,468,548 + BWI76_RS06990 0.49 +1.0
Or see this region's nucleotide sequence