Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02415 and BWI76_RS02420 are separated by 70 nucleotides BWI76_RS02420 and BWI76_RS02425 are separated by 163 nucleotides BWI76_RS02425 and BWI76_RS02430 are separated by 140 nucleotides BWI76_RS02430 and BWI76_RS02435 overlap by 4 nucleotides BWI76_RS02435 and BWI76_RS02440 are separated by 140 nucleotides BWI76_RS02440 and BWI76_RS02445 are separated by 10 nucleotides BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides
BWI76_RS02415: BWI76_RS02415 - elongation factor P, at 497,957 to 498,523
_RS02415
BWI76_RS02420: BWI76_RS02420 - entericidin EcnAB, at 498,594 to 498,728
_RS02420
BWI76_RS02425: BWI76_RS02425 - entericidin B, at 498,892 to 499,038
_RS02425
BWI76_RS02430: BWI76_RS02430 - multidrug transporter, at 499,179 to 499,496
_RS02430
BWI76_RS02435: BWI76_RS02435 - outer membrane lipoprotein Blc, at 499,493 to 500,023
_RS02435
BWI76_RS02440: BWI76_RS02440 - fumarate reductase subunit D, at 500,164 to 500,523
_RS02440
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
Position (kb) 499
500
501 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 498.557 kb on - strand at 498.561 kb on + strand at 498.563 kb on + strand at 498.569 kb on - strand at 498.573 kb on + strand at 498.573 kb on + strand at 498.640 kb on + strand, within BWI76_RS02420 at 498.640 kb on + strand, within BWI76_RS02420 at 498.648 kb on - strand, within BWI76_RS02420 at 498.656 kb on + strand, within BWI76_RS02420 at 498.729 kb on + strand at 498.740 kb on - strand at 498.765 kb on - strand at 498.826 kb on + strand at 498.871 kb on - strand at 498.890 kb on - strand at 498.925 kb on - strand, within BWI76_RS02425 at 498.938 kb on + strand, within BWI76_RS02425 at 498.938 kb on + strand, within BWI76_RS02425 at 498.938 kb on + strand, within BWI76_RS02425 at 498.938 kb on + strand, within BWI76_RS02425 at 498.938 kb on + strand, within BWI76_RS02425 at 498.946 kb on - strand, within BWI76_RS02425 at 498.946 kb on - strand, within BWI76_RS02425 at 498.951 kb on - strand, within BWI76_RS02425 at 498.962 kb on - strand, within BWI76_RS02425 at 498.962 kb on - strand, within BWI76_RS02425 at 498.962 kb on - strand, within BWI76_RS02425 at 499.020 kb on + strand, within BWI76_RS02425 at 499.060 kb on + strand at 499.148 kb on + strand at 499.196 kb on + strand at 499.202 kb on - strand at 499.211 kb on + strand, within BWI76_RS02430 at 499.211 kb on + strand, within BWI76_RS02430 at 499.219 kb on - strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.308 kb on + strand, within BWI76_RS02430 at 499.324 kb on - strand, within BWI76_RS02430 at 499.359 kb on + strand, within BWI76_RS02430 at 499.392 kb on - strand, within BWI76_RS02430 at 499.397 kb on - strand, within BWI76_RS02430 at 499.468 kb on + strand at 499.491 kb on + strand at 499.499 kb on - strand at 499.510 kb on + strand at 499.606 kb on + strand, within BWI76_RS02435 at 499.614 kb on - strand, within BWI76_RS02435 at 499.828 kb on + strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.934 kb on - strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 500.052 kb on + strand at 500.299 kb on - strand, within BWI76_RS02440 at 500.304 kb on + strand, within BWI76_RS02440 at 500.463 kb on - strand, within BWI76_RS02440 at 500.543 kb on - strand at 500.543 kb on - strand at 500.586 kb on + strand, within BWI76_RS02445 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 498,557 - -1.0 498,561 + +0.1 498,563 + -0.0 498,569 - -0.1 498,573 + +0.2 498,573 + +0.5 498,640 + BWI76_RS02420 0.34 -0.4 498,640 + BWI76_RS02420 0.34 +0.1 498,648 - BWI76_RS02420 0.40 +0.2 498,656 + BWI76_RS02420 0.46 -0.2 498,729 + +0.6 498,740 - -0.8 498,765 - -0.1 498,826 + -0.1 498,871 - -2.2 498,890 - -0.8 498,925 - BWI76_RS02425 0.22 -0.4 498,938 + BWI76_RS02425 0.31 -0.2 498,938 + BWI76_RS02425 0.31 +0.5 498,938 + BWI76_RS02425 0.31 -0.9 498,938 + BWI76_RS02425 0.31 -1.1 498,938 + BWI76_RS02425 0.31 -0.8 498,946 - BWI76_RS02425 0.37 +1.1 498,946 - BWI76_RS02425 0.37 -0.2 498,951 - BWI76_RS02425 0.40 +0.7 498,962 - BWI76_RS02425 0.48 -0.6 498,962 - BWI76_RS02425 0.48 +0.2 498,962 - BWI76_RS02425 0.48 -1.4 499,020 + BWI76_RS02425 0.87 -0.0 499,060 + +0.2 499,148 + -0.4 499,196 + +0.4 499,202 - +0.9 499,211 + BWI76_RS02430 0.10 +0.4 499,211 + BWI76_RS02430 0.10 +0.1 499,219 - BWI76_RS02430 0.13 -0.2 499,220 + BWI76_RS02430 0.13 -0.0 499,220 + BWI76_RS02430 0.13 +0.0 499,308 + BWI76_RS02430 0.41 +0.1 499,324 - BWI76_RS02430 0.46 -0.3 499,359 + BWI76_RS02430 0.57 +1.6 499,392 - BWI76_RS02430 0.67 -0.6 499,397 - BWI76_RS02430 0.69 +0.2 499,468 + -1.4 499,491 + +0.7 499,499 - -0.3 499,510 + +0.3 499,606 + BWI76_RS02435 0.21 -1.6 499,614 - BWI76_RS02435 0.23 -0.0 499,828 + BWI76_RS02435 0.63 +0.7 499,836 - BWI76_RS02435 0.65 +2.0 499,836 - BWI76_RS02435 0.65 +0.4 499,836 - BWI76_RS02435 0.65 +0.0 499,934 - BWI76_RS02435 0.83 -0.5 499,960 + BWI76_RS02435 0.88 +0.5 499,960 + BWI76_RS02435 0.88 +1.5 499,960 + BWI76_RS02435 0.88 +0.7 500,052 + +0.5 500,299 - BWI76_RS02440 0.38 -0.2 500,304 + BWI76_RS02440 0.39 +0.5 500,463 - BWI76_RS02440 0.83 -0.4 500,543 - -0.8 500,543 - +0.0 500,586 + BWI76_RS02445 0.13 +1.2 500,762 + BWI76_RS02445 0.58 +0.4 500,848 + BWI76_RS02445 0.79 -1.4 500,850 + BWI76_RS02445 0.80 -0.2 500,911 - +0.1 500,948 + -2.8 500,956 - +0.3 500,994 - +0.2
Or see this region's nucleotide sequence