Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01130 and BWI76_RS01135 are separated by 11 nucleotides BWI76_RS01135 and BWI76_RS01140 are separated by 240 nucleotides
BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245
_RS01130
BWI76_RS01135: BWI76_RS01135 - lipopolysaccharide N-acetylmannosaminouronosyltransferase, at 234,257 to 234,997
_RS01135
BWI76_RS01140: BWI76_RS01140 - amino acid permease, at 235,238 to 236,623
_RS01140
Position (kb) 234
235 Strain fitness (log2 ratio) -2
-1
0
1 at 234.278 kb on - strand at 234.297 kb on - strand at 234.607 kb on + strand, within BWI76_RS01135 at 234.644 kb on - strand, within BWI76_RS01135 at 234.795 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.828 kb on - strand, within BWI76_RS01135 at 234.839 kb on + strand, within BWI76_RS01135 at 234.986 kb on + strand at 235.121 kb on + strand at 235.121 kb on + strand at 235.121 kb on + strand at 235.124 kb on - strand at 235.129 kb on - strand at 235.183 kb on - strand at 235.185 kb on + strand at 235.188 kb on - strand at 235.200 kb on - strand at 235.210 kb on - strand at 235.216 kb on - strand at 235.216 kb on - strand at 235.216 kb on - strand at 235.218 kb on + strand at 235.227 kb on - strand at 235.298 kb on + strand at 235.346 kb on + strand at 235.585 kb on + strand, within BWI76_RS01140 at 235.701 kb on + strand, within BWI76_RS01140 at 235.710 kb on - strand, within BWI76_RS01140 at 235.742 kb on - strand, within BWI76_RS01140 at 235.755 kb on + strand, within BWI76_RS01140 at 235.869 kb on + strand, within BWI76_RS01140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 234,278 - +0.2 234,297 - +1.2 234,607 + BWI76_RS01135 0.47 -0.8 234,644 - BWI76_RS01135 0.52 -0.8 234,795 + BWI76_RS01135 0.73 -1.5 234,820 + BWI76_RS01135 0.76 -1.4 234,820 + BWI76_RS01135 0.76 -1.1 234,820 + BWI76_RS01135 0.76 -2.2 234,828 - BWI76_RS01135 0.77 -0.4 234,839 + BWI76_RS01135 0.79 +1.2 234,986 + -0.0 235,121 + +0.3 235,121 + -0.2 235,121 + +0.4 235,124 - -0.8 235,129 - -0.9 235,183 - +0.2 235,185 + -1.1 235,188 - -0.5 235,200 - -0.1 235,210 - +1.2 235,216 - +0.0 235,216 - -0.6 235,216 - +1.2 235,218 + +0.4 235,227 - +0.1 235,298 + -0.4 235,346 + +0.4 235,585 + BWI76_RS01140 0.25 -0.5 235,701 + BWI76_RS01140 0.33 +0.2 235,710 - BWI76_RS01140 0.34 -1.0 235,742 - BWI76_RS01140 0.36 -0.9 235,755 + BWI76_RS01140 0.37 +0.9 235,869 + BWI76_RS01140 0.46 +0.1
Or see this region's nucleotide sequence