Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides BWI76_RS00975 and BWI76_RS00980 are separated by 2 nucleotides
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
BWI76_RS00980: BWI76_RS00980 - PLP-dependent threonine dehydratase, at 200,147 to 201,691
_RS00980
Position (kb) 198
199
200
201 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970 at 198.044 kb on + strand, within BWI76_RS00970 at 198.112 kb on + strand, within BWI76_RS00970 at 198.168 kb on + strand at 198.297 kb on + strand at 198.442 kb on + strand at 198.442 kb on + strand at 198.543 kb on + strand, within BWI76_RS00975 at 198.551 kb on - strand, within BWI76_RS00975 at 198.608 kb on + strand, within BWI76_RS00975 at 198.636 kb on - strand, within BWI76_RS00975 at 198.648 kb on + strand, within BWI76_RS00975 at 198.849 kb on + strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 199.017 kb on - strand, within BWI76_RS00975 at 199.040 kb on + strand, within BWI76_RS00975 at 199.043 kb on - strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.234 kb on - strand, within BWI76_RS00975 at 199.740 kb on - strand, within BWI76_RS00975 at 199.806 kb on + strand, within BWI76_RS00975 at 199.877 kb on - strand, within BWI76_RS00975 at 199.960 kb on - strand at 200.009 kb on + strand at 200.062 kb on + strand at 200.070 kb on - strand at 200.192 kb on - strand at 200.207 kb on - strand at 200.207 kb on - strand at 200.229 kb on - strand at 200.244 kb on - strand at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.325 kb on + strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.333 kb on - strand, within BWI76_RS00980 at 200.558 kb on + strand, within BWI76_RS00980 at 200.652 kb on - strand, within BWI76_RS00980 at 200.792 kb on + strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.845 kb on - strand, within BWI76_RS00980 at 200.869 kb on - strand, within BWI76_RS00980 at 200.929 kb on + strand, within BWI76_RS00980
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 197,330 - -0.8 197,383 + -1.1 197,399 - BWI76_RS00970 0.10 -3.5 197,484 + BWI76_RS00970 0.19 -1.3 197,484 + BWI76_RS00970 0.19 -0.3 197,540 + BWI76_RS00970 0.25 -2.1 197,540 + BWI76_RS00970 0.25 -2.1 197,686 + BWI76_RS00970 0.41 -0.6 198,044 + BWI76_RS00970 0.80 +2.2 198,112 + BWI76_RS00970 0.87 -0.2 198,168 + +0.2 198,297 + -3.0 198,442 + -3.7 198,442 + -6.4 198,543 + BWI76_RS00975 0.13 -2.5 198,551 - BWI76_RS00975 0.14 -3.8 198,608 + BWI76_RS00975 0.17 -3.5 198,636 - BWI76_RS00975 0.18 -3.0 198,648 + BWI76_RS00975 0.19 -5.2 198,849 + BWI76_RS00975 0.30 -2.3 198,850 - BWI76_RS00975 0.30 -3.2 198,850 - BWI76_RS00975 0.30 -4.1 199,017 - BWI76_RS00975 0.39 -5.4 199,040 + BWI76_RS00975 0.40 -3.3 199,043 - BWI76_RS00975 0.40 -4.1 199,226 + BWI76_RS00975 0.50 -3.6 199,226 + BWI76_RS00975 0.50 -0.8 199,226 + BWI76_RS00975 0.50 -3.8 199,226 + BWI76_RS00975 0.50 -1.8 199,234 - BWI76_RS00975 0.51 -3.5 199,234 - BWI76_RS00975 0.51 -4.5 199,740 - BWI76_RS00975 0.78 +2.2 199,806 + BWI76_RS00975 0.82 -1.6 199,877 - BWI76_RS00975 0.86 -5.0 199,960 - -3.4 200,009 + -2.2 200,062 + -3.3 200,070 - -2.9 200,192 - +0.7 200,207 - -2.6 200,207 - -3.8 200,229 - -1.9 200,244 - -3.6 200,325 + BWI76_RS00980 0.12 -3.1 200,325 + BWI76_RS00980 0.12 -1.1 200,325 + BWI76_RS00980 0.12 -5.1 200,333 - BWI76_RS00980 0.12 -3.3 200,333 - BWI76_RS00980 0.12 -3.8 200,333 - BWI76_RS00980 0.12 -3.2 200,333 - BWI76_RS00980 0.12 -4.1 200,333 - BWI76_RS00980 0.12 -5.1 200,333 - BWI76_RS00980 0.12 -3.5 200,333 - BWI76_RS00980 0.12 -2.5 200,333 - BWI76_RS00980 0.12 -4.2 200,333 - BWI76_RS00980 0.12 -5.1 200,333 - BWI76_RS00980 0.12 -3.1 200,558 + BWI76_RS00980 0.27 -4.5 200,652 - BWI76_RS00980 0.33 -1.3 200,792 + BWI76_RS00980 0.42 -3.5 200,845 - BWI76_RS00980 0.45 +1.2 200,845 - BWI76_RS00980 0.45 -2.7 200,869 - BWI76_RS00980 0.47 -4.3 200,929 + BWI76_RS00980 0.51 -4.4
Or see this region's nucleotide sequence