Experiment: RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00380 and BWI76_RS00385 are separated by 258 nucleotides BWI76_RS00385 and BWI76_RS00390 are separated by 8 nucleotides
BWI76_RS00380: BWI76_RS00380 - oxygen-independent coproporphyrinogen III oxidase, at 79,246 to 80,619
_RS00380
BWI76_RS00385: BWI76_RS00385 - nitrogen assimilation regulatory protein, at 80,878 to 82,287
_RS00385
BWI76_RS00390: BWI76_RS00390 - two-component system sensor histidine kinase NtrB, at 82,296 to 83,345
_RS00390
Position (kb)
80
81
82
83 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 79.938 kb on + strand, within BWI76_RS00380 at 79.946 kb on - strand, within BWI76_RS00380 at 80.038 kb on - strand, within BWI76_RS00380 at 80.157 kb on - strand, within BWI76_RS00380 at 80.259 kb on + strand, within BWI76_RS00380 at 80.422 kb on + strand, within BWI76_RS00380 at 80.524 kb on + strand at 80.636 kb on + strand at 80.686 kb on - strand at 80.720 kb on - strand at 80.724 kb on + strand at 80.812 kb on - strand at 80.913 kb on + strand at 80.959 kb on + strand at 80.983 kb on + strand at 80.989 kb on - strand at 81.125 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.163 kb on + strand, within BWI76_RS00385 at 81.171 kb on - strand, within BWI76_RS00385 at 81.221 kb on - strand, within BWI76_RS00385 at 81.241 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.432 kb on + strand, within BWI76_RS00385 at 81.600 kb on + strand, within BWI76_RS00385 at 81.652 kb on - strand, within BWI76_RS00385 at 81.934 kb on - strand, within BWI76_RS00385 at 82.010 kb on + strand, within BWI76_RS00385 at 82.010 kb on - strand, within BWI76_RS00385 at 82.201 kb on + strand at 82.201 kb on + strand at 82.209 kb on - strand at 82.209 kb on - strand at 82.255 kb on - strand at 82.334 kb on + strand at 82.334 kb on + strand at 82.400 kb on + strand at 82.493 kb on - strand, within BWI76_RS00390 at 82.861 kb on + strand, within BWI76_RS00390 at 82.889 kb on + strand, within BWI76_RS00390 at 83.033 kb on + strand, within BWI76_RS00390 at 83.040 kb on + strand, within BWI76_RS00390 at 83.142 kb on + strand, within BWI76_RS00390 at 83.186 kb on - strand, within BWI76_RS00390
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Microbacterium sp. AnTP90 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 79,938 + BWI76_RS00380 0.50 +0.2 79,946 - BWI76_RS00380 0.51 +0.7 80,038 - BWI76_RS00380 0.58 +0.3 80,157 - BWI76_RS00380 0.66 -1.1 80,259 + BWI76_RS00380 0.74 -0.5 80,422 + BWI76_RS00380 0.86 +1.3 80,524 + -1.2 80,636 + -0.3 80,686 - -3.2 80,720 - +0.5 80,724 + +0.1 80,812 - +1.6 80,913 + -0.6 80,959 + -2.3 80,983 + -0.4 80,989 - -0.3 81,125 + BWI76_RS00385 0.18 -2.2 81,163 + BWI76_RS00385 0.20 -1.1 81,163 + BWI76_RS00385 0.20 -0.4 81,163 + BWI76_RS00385 0.20 -1.8 81,171 - BWI76_RS00385 0.21 -1.7 81,221 - BWI76_RS00385 0.24 -3.3 81,241 + BWI76_RS00385 0.26 -0.6 81,432 + BWI76_RS00385 0.39 -1.4 81,432 + BWI76_RS00385 0.39 -1.7 81,600 + BWI76_RS00385 0.51 -0.5 81,652 - BWI76_RS00385 0.55 -0.7 81,934 - BWI76_RS00385 0.75 -0.1 82,010 + BWI76_RS00385 0.80 -1.2 82,010 - BWI76_RS00385 0.80 -2.3 82,201 + -0.2 82,201 + +0.3 82,209 - -1.9 82,209 - -1.7 82,255 - -0.5 82,334 + -1.2 82,334 + -1.7 82,400 + -1.1 82,493 - BWI76_RS00390 0.19 -1.7 82,861 + BWI76_RS00390 0.54 -1.1 82,889 + BWI76_RS00390 0.56 -2.4 83,033 + BWI76_RS00390 0.70 +0.3 83,040 + BWI76_RS00390 0.71 -1.7 83,142 + BWI76_RS00390 0.81 -1.3 83,186 - BWI76_RS00390 0.85 -0.7
Or see this region's nucleotide sequence