Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14945 and BWI76_RS14950 are separated by 4 nucleotides BWI76_RS14950 and BWI76_RS14955 are separated by 3 nucleotides
BWI76_RS14945: BWI76_RS14945 - PTS system, cellobiose-specific IIC component, at 3,131,958 to 3,133,277
_RS14945
BWI76_RS14950: BWI76_RS14950 - glycosylasparaginase, at 3,133,282 to 3,134,235
_RS14950
BWI76_RS14955: BWI76_RS14955 - peptidase M17 leucyl aminopeptidase domain-containing protein, at 3,134,239 to 3,135,594
_RS14955
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-1
0
1 at 3132.620 kb on + strand, within BWI76_RS14945 at 3132.657 kb on + strand, within BWI76_RS14945 at 3133.014 kb on + strand, within BWI76_RS14945 at 3133.024 kb on - strand, within BWI76_RS14945 at 3133.074 kb on - strand, within BWI76_RS14945 at 3133.131 kb on + strand, within BWI76_RS14945 at 3133.370 kb on + strand at 3133.421 kb on - strand at 3133.637 kb on + strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3134.204 kb on - strand at 3134.232 kb on - strand at 3134.529 kb on - strand, within BWI76_RS14955 at 3134.561 kb on + strand, within BWI76_RS14955 at 3135.083 kb on + strand, within BWI76_RS14955 at 3135.123 kb on + strand, within BWI76_RS14955
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,132,620 + BWI76_RS14945 0.50 +0.4 3,132,657 + BWI76_RS14945 0.53 +0.6 3,133,014 + BWI76_RS14945 0.80 +0.8 3,133,024 - BWI76_RS14945 0.81 +0.5 3,133,074 - BWI76_RS14945 0.85 +0.4 3,133,131 + BWI76_RS14945 0.89 +0.6 3,133,370 + -0.6 3,133,421 - -0.0 3,133,637 + BWI76_RS14950 0.37 +0.5 3,133,851 - BWI76_RS14950 0.60 +0.1 3,133,851 - BWI76_RS14950 0.60 +0.7 3,134,204 - +0.4 3,134,232 - +1.0 3,134,529 - BWI76_RS14955 0.21 +0.4 3,134,561 + BWI76_RS14955 0.24 +0.0 3,135,083 + BWI76_RS14955 0.62 +0.5 3,135,123 + BWI76_RS14955 0.65 +1.0
Or see this region's nucleotide sequence