Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12635 and BWI76_RS12640 are separated by 216 nucleotides BWI76_RS12640 and BWI76_RS12645 are separated by 125 nucleotides BWI76_RS12645 and BWI76_RS12650 are separated by 199 nucleotides
BWI76_RS12635: BWI76_RS12635 - cold-shock protein, at 2,637,768 to 2,637,956
_RS12635
BWI76_RS12640: BWI76_RS12640 - hypothetical protein, at 2,638,173 to 2,638,802
_RS12640
BWI76_RS12645: BWI76_RS12645 - membrane protein, at 2,638,928 to 2,639,509
_RS12645
BWI76_RS12650: BWI76_RS12650 - membrane protein, at 2,639,709 to 2,640,272
_RS12650
Position (kb)
2638
2639 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2637.249 kb on + strand at 2637.275 kb on + strand at 2637.299 kb on - strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.375 kb on + strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.383 kb on - strand at 2637.394 kb on + strand at 2637.397 kb on - strand at 2637.432 kb on + strand at 2637.471 kb on - strand at 2637.478 kb on - strand at 2637.520 kb on - strand at 2637.533 kb on + strand at 2637.589 kb on + strand at 2637.589 kb on + strand at 2637.602 kb on + strand at 2637.610 kb on - strand at 2637.613 kb on + strand at 2637.613 kb on + strand at 2637.646 kb on + strand at 2637.656 kb on + strand at 2637.717 kb on + strand at 2637.758 kb on + strand at 2637.764 kb on + strand at 2637.772 kb on - strand at 2637.772 kb on - strand at 2637.772 kb on - strand at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on + strand, within BWI76_RS12635 at 2637.802 kb on - strand, within BWI76_RS12635 at 2637.810 kb on + strand, within BWI76_RS12635 at 2637.810 kb on - strand, within BWI76_RS12635 at 2637.810 kb on - strand at 2637.810 kb on - strand, within BWI76_RS12635 at 2637.813 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.815 kb on + strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.823 kb on - strand, within BWI76_RS12635 at 2637.825 kb on - strand, within BWI76_RS12635 at 2638.023 kb on + strand at 2638.094 kb on - strand at 2638.097 kb on + strand at 2638.173 kb on + strand at 2638.214 kb on + strand at 2638.214 kb on + strand at 2638.285 kb on - strand, within BWI76_RS12640 at 2638.337 kb on + strand, within BWI76_RS12640 at 2638.344 kb on + strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.352 kb on - strand, within BWI76_RS12640 at 2638.401 kb on - strand, within BWI76_RS12640 at 2638.423 kb on + strand, within BWI76_RS12640 at 2638.423 kb on + strand, within BWI76_RS12640 at 2638.436 kb on + strand, within BWI76_RS12640 at 2638.450 kb on + strand, within BWI76_RS12640 at 2638.458 kb on - strand, within BWI76_RS12640 at 2638.458 kb on - strand at 2638.546 kb on - strand, within BWI76_RS12640 at 2638.594 kb on - strand, within BWI76_RS12640 at 2638.638 kb on - strand, within BWI76_RS12640 at 2639.007 kb on - strand, within BWI76_RS12645 at 2639.008 kb on - strand, within BWI76_RS12645 at 2639.039 kb on + strand, within BWI76_RS12645 at 2639.045 kb on + strand, within BWI76_RS12645 at 2639.050 kb on + strand, within BWI76_RS12645 at 2639.089 kb on + strand, within BWI76_RS12645 at 2639.125 kb on + strand, within BWI76_RS12645 at 2639.185 kb on + strand, within BWI76_RS12645 at 2639.244 kb on + strand, within BWI76_RS12645 at 2639.244 kb on + strand, within BWI76_RS12645 at 2639.244 kb on + strand, within BWI76_RS12645 at 2639.244 kb on + strand, within BWI76_RS12645 at 2639.244 kb on + strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.252 kb on - strand, within BWI76_RS12645 at 2639.285 kb on + strand, within BWI76_RS12645 at 2639.290 kb on + strand, within BWI76_RS12645 at 2639.320 kb on - strand, within BWI76_RS12645 at 2639.367 kb on + strand, within BWI76_RS12645 at 2639.403 kb on + strand, within BWI76_RS12645 at 2639.403 kb on - strand, within BWI76_RS12645 at 2639.419 kb on + strand, within BWI76_RS12645 at 2639.450 kb on + strand, within BWI76_RS12645 at 2639.458 kb on - strand at 2639.468 kb on + strand at 2639.472 kb on + strand at 2639.480 kb on - strand at 2639.482 kb on + strand at 2639.565 kb on + strand at 2639.578 kb on - strand at 2639.598 kb on - strand at 2639.664 kb on + strand at 2639.740 kb on + strand at 2639.743 kb on - strand at 2639.745 kb on + strand at 2639.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,637,249 + -0.6 2,637,275 + +1.0 2,637,299 - +0.5 2,637,375 + -2.6 2,637,375 + +1.4 2,637,375 + -0.4 2,637,375 + +1.0 2,637,375 + -0.3 2,637,375 + +1.0 2,637,375 + -1.6 2,637,375 + +0.3 2,637,375 + -0.8 2,637,375 + -0.2 2,637,375 + +0.6 2,637,375 + -0.5 2,637,375 + -0.3 2,637,375 + -0.5 2,637,375 + +0.7 2,637,375 + -0.8 2,637,383 - +1.0 2,637,383 - -1.6 2,637,383 - +1.9 2,637,383 - +1.4 2,637,383 - +1.0 2,637,383 - -0.8 2,637,394 + -0.2 2,637,397 - +0.1 2,637,432 + +0.2 2,637,471 - +0.8 2,637,478 - +0.4 2,637,520 - +2.2 2,637,533 + +0.4 2,637,589 + +0.0 2,637,589 + -0.6 2,637,602 + -2.6 2,637,610 - -0.6 2,637,613 + +0.6 2,637,613 + -1.0 2,637,646 + -2.0 2,637,656 + +0.9 2,637,717 + -0.2 2,637,758 + +0.1 2,637,764 + +0.2 2,637,772 - -0.1 2,637,772 - +1.3 2,637,772 - +1.2 2,637,802 + BWI76_RS12635 0.18 -0.6 2,637,802 + BWI76_RS12635 0.18 -1.0 2,637,802 + BWI76_RS12635 0.18 -1.4 2,637,802 - BWI76_RS12635 0.18 +0.2 2,637,810 + BWI76_RS12635 0.22 +0.3 2,637,810 - BWI76_RS12635 0.22 +0.2 2,637,810 - +2.0 2,637,810 - BWI76_RS12635 0.22 -0.7 2,637,813 + BWI76_RS12635 0.24 +0.0 2,637,815 + BWI76_RS12635 0.25 -3.1 2,637,815 + BWI76_RS12635 0.25 +0.0 2,637,815 + BWI76_RS12635 0.25 -0.1 2,637,815 + BWI76_RS12635 0.25 +0.0 2,637,815 + BWI76_RS12635 0.25 -0.1 2,637,815 + +2.0 2,637,815 + BWI76_RS12635 0.25 -0.2 2,637,815 + +0.3 2,637,815 + BWI76_RS12635 0.25 +0.4 2,637,815 + BWI76_RS12635 0.25 -1.2 2,637,815 + +1.0 2,637,815 + BWI76_RS12635 0.25 +0.4 2,637,815 + +0.0 2,637,815 + BWI76_RS12635 0.25 -0.9 2,637,815 + BWI76_RS12635 0.25 +0.5 2,637,815 + BWI76_RS12635 0.25 +0.6 2,637,815 + BWI76_RS12635 0.25 +0.0 2,637,815 + BWI76_RS12635 0.25 +0.6 2,637,815 + BWI76_RS12635 0.25 -2.1 2,637,815 + BWI76_RS12635 0.25 +1.3 2,637,815 + BWI76_RS12635 0.25 -0.3 2,637,815 + BWI76_RS12635 0.25 +2.4 2,637,815 + BWI76_RS12635 0.25 -1.3 2,637,823 - BWI76_RS12635 0.29 +1.0 2,637,823 - BWI76_RS12635 0.29 +0.0 2,637,823 - BWI76_RS12635 0.29 +1.0 2,637,825 - BWI76_RS12635 0.30 -1.6 2,638,023 + +1.0 2,638,094 - +1.0 2,638,097 + -0.8 2,638,173 + -0.6 2,638,214 + +0.2 2,638,214 + +1.0 2,638,285 - BWI76_RS12640 0.18 -0.2 2,638,337 + BWI76_RS12640 0.26 -0.2 2,638,344 + BWI76_RS12640 0.27 -0.7 2,638,352 - BWI76_RS12640 0.28 -1.3 2,638,352 - BWI76_RS12640 0.28 -0.3 2,638,352 - BWI76_RS12640 0.28 -0.6 2,638,401 - BWI76_RS12640 0.36 -2.3 2,638,423 + BWI76_RS12640 0.40 -0.9 2,638,423 + BWI76_RS12640 0.40 -0.6 2,638,436 + BWI76_RS12640 0.42 -1.8 2,638,450 + BWI76_RS12640 0.44 +1.0 2,638,458 - BWI76_RS12640 0.45 -1.1 2,638,458 - -0.6 2,638,546 - BWI76_RS12640 0.59 +0.4 2,638,594 - BWI76_RS12640 0.67 -0.8 2,638,638 - BWI76_RS12640 0.74 +0.4 2,639,007 - BWI76_RS12645 0.14 +0.1 2,639,008 - BWI76_RS12645 0.14 +1.0 2,639,039 + BWI76_RS12645 0.19 +0.1 2,639,045 + BWI76_RS12645 0.20 +0.3 2,639,050 + BWI76_RS12645 0.21 +0.4 2,639,089 + BWI76_RS12645 0.28 +1.0 2,639,125 + BWI76_RS12645 0.34 -0.4 2,639,185 + BWI76_RS12645 0.44 -0.6 2,639,244 + BWI76_RS12645 0.54 +1.6 2,639,244 + BWI76_RS12645 0.54 +1.2 2,639,244 + BWI76_RS12645 0.54 +0.3 2,639,244 + BWI76_RS12645 0.54 +0.4 2,639,244 + BWI76_RS12645 0.54 +0.8 2,639,252 - BWI76_RS12645 0.56 +0.1 2,639,252 - BWI76_RS12645 0.56 -0.8 2,639,252 - BWI76_RS12645 0.56 +1.4 2,639,252 - BWI76_RS12645 0.56 +0.3 2,639,252 - BWI76_RS12645 0.56 +1.2 2,639,252 - BWI76_RS12645 0.56 -0.3 2,639,285 + BWI76_RS12645 0.61 +0.4 2,639,290 + BWI76_RS12645 0.62 +0.5 2,639,320 - BWI76_RS12645 0.67 +0.2 2,639,367 + BWI76_RS12645 0.75 -0.5 2,639,403 + BWI76_RS12645 0.82 +0.2 2,639,403 - BWI76_RS12645 0.82 +0.4 2,639,419 + BWI76_RS12645 0.84 -0.2 2,639,450 + BWI76_RS12645 0.90 +0.4 2,639,458 - +1.0 2,639,468 + +1.0 2,639,472 + +1.4 2,639,480 - +1.5 2,639,482 + +0.8 2,639,565 + -1.3 2,639,578 - -2.3 2,639,598 - -1.0 2,639,664 + -0.6 2,639,740 + +1.0 2,639,743 - +1.0 2,639,745 + -0.2 2,639,761 + -0.2
Or see this region's nucleotide sequence