Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04285 and BWI76_RS04290 are separated by 88 nucleotides BWI76_RS04290 and BWI76_RS04295 are separated by 27 nucleotides BWI76_RS04295 and BWI76_RS04300 are separated by 385 nucleotides
BWI76_RS04285: BWI76_RS04285 - hypothetical protein, at 882,857 to 883,423
_RS04285
BWI76_RS04290: BWI76_RS04290 - hypothetical protein, at 883,512 to 884,261
_RS04290
BWI76_RS04295: BWI76_RS04295 - hypothetical protein, at 884,289 to 884,693
_RS04295
BWI76_RS04300: BWI76_RS04300 - molecular chaperone DnaK, at 885,079 to 886,995
_RS04300
Position (kb) 884
885 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 883.318 kb on + strand, within BWI76_RS04285 at 883.350 kb on - strand, within BWI76_RS04285 at 883.365 kb on - strand, within BWI76_RS04285 at 883.393 kb on - strand at 883.408 kb on + strand at 883.416 kb on - strand at 883.472 kb on - strand at 883.510 kb on + strand at 883.574 kb on + strand at 883.582 kb on - strand at 883.582 kb on - strand at 883.590 kb on + strand, within BWI76_RS04290 at 883.636 kb on + strand, within BWI76_RS04290 at 883.644 kb on - strand, within BWI76_RS04290 at 883.676 kb on + strand, within BWI76_RS04290 at 883.771 kb on + strand, within BWI76_RS04290 at 883.775 kb on + strand, within BWI76_RS04290 at 883.778 kb on - strand, within BWI76_RS04290 at 883.779 kb on - strand, within BWI76_RS04290 at 883.789 kb on - strand, within BWI76_RS04290 at 883.808 kb on + strand, within BWI76_RS04290 at 883.814 kb on + strand, within BWI76_RS04290 at 883.915 kb on - strand, within BWI76_RS04290 at 883.977 kb on - strand, within BWI76_RS04290 at 884.012 kb on + strand, within BWI76_RS04290 at 884.046 kb on + strand, within BWI76_RS04290 at 884.054 kb on - strand, within BWI76_RS04290 at 884.054 kb on - strand, within BWI76_RS04290 at 884.062 kb on + strand, within BWI76_RS04290 at 884.112 kb on - strand, within BWI76_RS04290 at 884.112 kb on - strand, within BWI76_RS04290 at 884.127 kb on + strand, within BWI76_RS04290 at 884.133 kb on - strand, within BWI76_RS04290 at 884.135 kb on - strand, within BWI76_RS04290 at 884.138 kb on + strand, within BWI76_RS04290 at 884.138 kb on + strand, within BWI76_RS04290 at 884.140 kb on + strand, within BWI76_RS04290 at 884.140 kb on + strand, within BWI76_RS04290 at 884.140 kb on + strand, within BWI76_RS04290 at 884.140 kb on + strand, within BWI76_RS04290 at 884.146 kb on - strand, within BWI76_RS04290 at 884.148 kb on - strand, within BWI76_RS04290 at 884.148 kb on - strand, within BWI76_RS04290 at 884.148 kb on - strand, within BWI76_RS04290 at 884.148 kb on - strand, within BWI76_RS04290 at 884.148 kb on - strand, within BWI76_RS04290 at 884.151 kb on + strand, within BWI76_RS04290 at 884.185 kb on - strand, within BWI76_RS04290 at 884.227 kb on - strand at 884.227 kb on - strand at 884.231 kb on + strand at 884.239 kb on - strand at 884.239 kb on - strand at 884.239 kb on - strand at 884.286 kb on + strand at 884.294 kb on - strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.321 kb on + strand at 884.323 kb on + strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.329 kb on - strand at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.330 kb on + strand, within BWI76_RS04295 at 884.331 kb on - strand at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.338 kb on - strand, within BWI76_RS04295 at 884.370 kb on + strand at 884.380 kb on + strand, within BWI76_RS04295 at 884.382 kb on - strand, within BWI76_RS04295 at 884.395 kb on + strand, within BWI76_RS04295 at 884.397 kb on - strand, within BWI76_RS04295 at 884.397 kb on - strand, within BWI76_RS04295 at 884.397 kb on - strand, within BWI76_RS04295 at 884.397 kb on - strand, within BWI76_RS04295 at 884.419 kb on - strand, within BWI76_RS04295 at 884.423 kb on + strand, within BWI76_RS04295 at 884.431 kb on + strand, within BWI76_RS04295 at 884.431 kb on - strand, within BWI76_RS04295 at 884.431 kb on - strand, within BWI76_RS04295 at 884.431 kb on - strand, within BWI76_RS04295 at 884.443 kb on + strand, within BWI76_RS04295 at 884.443 kb on + strand, within BWI76_RS04295 at 884.443 kb on + strand, within BWI76_RS04295 at 884.447 kb on + strand, within BWI76_RS04295 at 884.447 kb on + strand, within BWI76_RS04295 at 884.447 kb on + strand, within BWI76_RS04295 at 884.448 kb on + strand, within BWI76_RS04295 at 884.448 kb on + strand, within BWI76_RS04295 at 884.453 kb on + strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.455 kb on - strand, within BWI76_RS04295 at 884.456 kb on - strand, within BWI76_RS04295 at 884.456 kb on - strand, within BWI76_RS04295 at 884.456 kb on - strand, within BWI76_RS04295 at 884.456 kb on - strand, within BWI76_RS04295 at 884.463 kb on + strand, within BWI76_RS04295 at 884.463 kb on + strand, within BWI76_RS04295 at 884.466 kb on - strand, within BWI76_RS04295 at 884.466 kb on - strand, within BWI76_RS04295 at 884.471 kb on - strand, within BWI76_RS04295 at 884.475 kb on + strand, within BWI76_RS04295 at 884.476 kb on + strand, within BWI76_RS04295 at 884.479 kb on - strand, within BWI76_RS04295 at 884.526 kb on - strand at 884.526 kb on - strand, within BWI76_RS04295 at 884.526 kb on - strand, within BWI76_RS04295 at 884.558 kb on + strand, within BWI76_RS04295 at 884.558 kb on + strand, within BWI76_RS04295 at 884.558 kb on + strand, within BWI76_RS04295 at 884.559 kb on + strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.566 kb on - strand, within BWI76_RS04295 at 884.572 kb on - strand, within BWI76_RS04295 at 884.572 kb on - strand, within BWI76_RS04295 at 884.572 kb on - strand, within BWI76_RS04295 at 884.603 kb on - strand, within BWI76_RS04295 at 884.603 kb on - strand, within BWI76_RS04295 at 884.612 kb on - strand, within BWI76_RS04295 at 884.630 kb on + strand, within BWI76_RS04295 at 884.635 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.640 kb on + strand, within BWI76_RS04295 at 884.648 kb on - strand, within BWI76_RS04295 at 884.648 kb on - strand, within BWI76_RS04295 at 884.648 kb on - strand, within BWI76_RS04295 at 884.648 kb on - strand, within BWI76_RS04295 at 884.652 kb on + strand, within BWI76_RS04295 at 884.653 kb on + strand at 884.658 kb on - strand at 884.658 kb on - strand at 884.829 kb on + strand at 884.837 kb on - strand at 884.856 kb on + strand at 884.863 kb on + strand at 884.863 kb on + strand at 884.868 kb on + strand at 884.876 kb on + strand at 884.876 kb on - strand at 884.879 kb on + strand at 884.879 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.881 kb on + strand at 884.887 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.889 kb on - strand at 884.899 kb on - strand at 884.936 kb on + strand at 884.936 kb on + strand at 884.944 kb on - strand at 884.944 kb on - strand at 884.948 kb on + strand at 884.954 kb on - strand at 884.957 kb on - strand at 884.972 kb on + strand at 884.994 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 883,318 + BWI76_RS04285 0.81 -0.7 883,350 - BWI76_RS04285 0.87 -1.5 883,365 - BWI76_RS04285 0.90 +0.5 883,393 - +0.1 883,408 + -0.2 883,416 - +0.7 883,472 - -0.1 883,510 + -0.7 883,574 + -2.1 883,582 - +0.1 883,582 - -1.6 883,590 + BWI76_RS04290 0.10 +0.3 883,636 + BWI76_RS04290 0.17 +0.6 883,644 - BWI76_RS04290 0.18 -0.2 883,676 + BWI76_RS04290 0.22 +0.3 883,771 + BWI76_RS04290 0.35 +0.2 883,775 + BWI76_RS04290 0.35 +0.5 883,778 - BWI76_RS04290 0.35 +1.3 883,779 - BWI76_RS04290 0.36 +0.5 883,789 - BWI76_RS04290 0.37 -0.0 883,808 + BWI76_RS04290 0.39 -1.5 883,814 + BWI76_RS04290 0.40 +0.1 883,915 - BWI76_RS04290 0.54 -0.3 883,977 - BWI76_RS04290 0.62 +0.8 884,012 + BWI76_RS04290 0.67 -0.5 884,046 + BWI76_RS04290 0.71 +1.1 884,054 - BWI76_RS04290 0.72 +0.1 884,054 - BWI76_RS04290 0.72 +1.1 884,062 + BWI76_RS04290 0.73 -0.5 884,112 - BWI76_RS04290 0.80 +0.6 884,112 - BWI76_RS04290 0.80 +0.4 884,127 + BWI76_RS04290 0.82 -1.3 884,133 - BWI76_RS04290 0.83 -1.5 884,135 - BWI76_RS04290 0.83 +0.2 884,138 + BWI76_RS04290 0.83 +1.2 884,138 + BWI76_RS04290 0.83 -0.3 884,140 + BWI76_RS04290 0.84 +0.8 884,140 + BWI76_RS04290 0.84 +1.1 884,140 + BWI76_RS04290 0.84 +0.2 884,140 + BWI76_RS04290 0.84 -3.2 884,146 - BWI76_RS04290 0.85 -0.9 884,148 - BWI76_RS04290 0.85 -0.3 884,148 - BWI76_RS04290 0.85 +0.9 884,148 - BWI76_RS04290 0.85 -0.2 884,148 - BWI76_RS04290 0.85 +0.7 884,148 - BWI76_RS04290 0.85 -0.1 884,151 + BWI76_RS04290 0.85 +0.1 884,185 - BWI76_RS04290 0.90 -1.2 884,227 - -1.4 884,227 - +0.3 884,231 + -0.6 884,239 - -1.5 884,239 - +1.1 884,239 - -0.5 884,286 + -0.3 884,294 - -0.5 884,321 + +1.1 884,321 + -0.4 884,321 + +1.1 884,321 + -0.2 884,321 + +0.0 884,321 + +0.4 884,321 + -0.3 884,321 + +0.5 884,323 + +0.4 884,329 - +0.5 884,329 - +0.1 884,329 - -0.6 884,329 - +0.4 884,329 - -0.5 884,329 - -0.3 884,329 - +0.9 884,329 - -0.5 884,329 - -2.1 884,329 - -1.0 884,330 + BWI76_RS04295 0.10 -0.6 884,330 + BWI76_RS04295 0.10 -0.4 884,330 + BWI76_RS04295 0.10 -1.2 884,330 + BWI76_RS04295 0.10 -1.5 884,330 + BWI76_RS04295 0.10 -0.3 884,330 + BWI76_RS04295 0.10 +0.1 884,330 + BWI76_RS04295 0.10 -2.4 884,330 + BWI76_RS04295 0.10 +0.1 884,330 + BWI76_RS04295 0.10 -0.1 884,331 - -0.5 884,338 - BWI76_RS04295 0.12 +0.1 884,338 - BWI76_RS04295 0.12 -0.1 884,338 - BWI76_RS04295 0.12 -2.3 884,338 - BWI76_RS04295 0.12 -0.2 884,338 - BWI76_RS04295 0.12 +0.7 884,338 - BWI76_RS04295 0.12 -0.9 884,338 - BWI76_RS04295 0.12 -0.9 884,338 - BWI76_RS04295 0.12 -0.3 884,338 - BWI76_RS04295 0.12 -0.1 884,338 - BWI76_RS04295 0.12 +0.2 884,338 - BWI76_RS04295 0.12 -0.3 884,338 - BWI76_RS04295 0.12 -0.4 884,370 + +1.1 884,380 + BWI76_RS04295 0.22 +0.1 884,382 - BWI76_RS04295 0.23 -0.8 884,395 + BWI76_RS04295 0.26 +0.8 884,397 - BWI76_RS04295 0.27 -2.1 884,397 - BWI76_RS04295 0.27 -0.2 884,397 - BWI76_RS04295 0.27 -0.0 884,397 - BWI76_RS04295 0.27 -1.9 884,419 - BWI76_RS04295 0.32 -0.3 884,423 + BWI76_RS04295 0.33 +1.0 884,431 + BWI76_RS04295 0.35 +0.2 884,431 - BWI76_RS04295 0.35 -0.7 884,431 - BWI76_RS04295 0.35 -1.6 884,431 - BWI76_RS04295 0.35 +0.8 884,443 + BWI76_RS04295 0.38 +1.1 884,443 + BWI76_RS04295 0.38 -1.3 884,443 + BWI76_RS04295 0.38 -0.5 884,447 + BWI76_RS04295 0.39 -0.6 884,447 + BWI76_RS04295 0.39 -1.1 884,447 + BWI76_RS04295 0.39 +0.8 884,448 + BWI76_RS04295 0.39 -0.0 884,448 + BWI76_RS04295 0.39 -0.4 884,453 + BWI76_RS04295 0.40 -0.5 884,455 - BWI76_RS04295 0.41 -1.5 884,455 - BWI76_RS04295 0.41 -0.3 884,455 - BWI76_RS04295 0.41 +0.4 884,455 - BWI76_RS04295 0.41 +0.2 884,455 - BWI76_RS04295 0.41 +0.6 884,455 - BWI76_RS04295 0.41 +0.2 884,456 - BWI76_RS04295 0.41 -2.3 884,456 - BWI76_RS04295 0.41 +0.0 884,456 - BWI76_RS04295 0.41 -0.5 884,456 - BWI76_RS04295 0.41 +0.2 884,463 + BWI76_RS04295 0.43 -0.5 884,463 + BWI76_RS04295 0.43 -1.1 884,466 - BWI76_RS04295 0.44 -0.5 884,466 - BWI76_RS04295 0.44 +1.1 884,471 - BWI76_RS04295 0.45 -1.9 884,475 + BWI76_RS04295 0.46 -0.7 884,476 + BWI76_RS04295 0.46 -0.7 884,479 - BWI76_RS04295 0.47 -0.8 884,526 - +0.7 884,526 - BWI76_RS04295 0.59 -0.0 884,526 - BWI76_RS04295 0.59 -1.9 884,558 + BWI76_RS04295 0.66 -0.5 884,558 + BWI76_RS04295 0.66 -1.3 884,558 + BWI76_RS04295 0.66 +0.1 884,559 + BWI76_RS04295 0.67 +0.1 884,566 - BWI76_RS04295 0.68 -0.1 884,566 - BWI76_RS04295 0.68 -0.0 884,566 - BWI76_RS04295 0.68 -0.3 884,566 - BWI76_RS04295 0.68 -2.8 884,566 - BWI76_RS04295 0.68 +0.5 884,566 - BWI76_RS04295 0.68 -0.4 884,566 - BWI76_RS04295 0.68 +0.1 884,566 - BWI76_RS04295 0.68 -3.8 884,566 - BWI76_RS04295 0.68 +0.9 884,566 - BWI76_RS04295 0.68 -0.9 884,566 - BWI76_RS04295 0.68 +1.1 884,566 - BWI76_RS04295 0.68 +0.5 884,566 - BWI76_RS04295 0.68 +0.0 884,572 - BWI76_RS04295 0.70 -0.9 884,572 - BWI76_RS04295 0.70 -0.6 884,572 - BWI76_RS04295 0.70 +1.2 884,603 - BWI76_RS04295 0.78 -0.4 884,603 - BWI76_RS04295 0.78 +0.1 884,612 - BWI76_RS04295 0.80 -0.5 884,630 + BWI76_RS04295 0.84 +1.1 884,635 + BWI76_RS04295 0.85 -1.5 884,640 + BWI76_RS04295 0.87 -0.7 884,640 + BWI76_RS04295 0.87 -0.2 884,640 + BWI76_RS04295 0.87 -0.9 884,640 + BWI76_RS04295 0.87 -0.5 884,640 + BWI76_RS04295 0.87 +0.5 884,640 + BWI76_RS04295 0.87 +0.3 884,640 + BWI76_RS04295 0.87 -1.3 884,648 - BWI76_RS04295 0.89 -0.3 884,648 - BWI76_RS04295 0.89 -0.9 884,648 - BWI76_RS04295 0.89 -2.3 884,648 - BWI76_RS04295 0.89 +1.1 884,652 + BWI76_RS04295 0.90 -0.7 884,653 + -2.3 884,658 - -0.9 884,658 - +1.1 884,829 + -0.2 884,837 - +0.1 884,856 + +1.1 884,863 + -1.0 884,863 + -0.4 884,868 + -1.3 884,876 + -1.3 884,876 - +1.1 884,879 + +0.5 884,879 + +0.0 884,881 + -0.1 884,881 + +0.0 884,881 + -0.2 884,881 + -0.5 884,881 + -0.7 884,881 + +0.9 884,881 + +0.5 884,881 + -1.0 884,881 + +0.8 884,881 + +2.4 884,887 - +0.4 884,889 - -0.3 884,889 - -1.7 884,889 - -0.2 884,889 - +1.0 884,889 - +0.1 884,889 - +0.7 884,889 - +1.3 884,889 - -0.1 884,889 - +1.3 884,889 - +2.1 884,889 - +0.6 884,899 - +0.1 884,936 + +1.7 884,936 + -0.7 884,944 - -1.2 884,944 - -0.8 884,948 + +1.2 884,954 - -1.3 884,957 - +0.2 884,972 + +0.7 884,994 + -0.9
Or see this region's nucleotide sequence