Experiment: RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03950 and BWI76_RS03955 are separated by 99 nucleotides BWI76_RS03955 and BWI76_RS03960 are separated by 14 nucleotides BWI76_RS03960 and BWI76_RS03965 are separated by 16 nucleotides BWI76_RS03965 and BWI76_RS03970 overlap by 23 nucleotides
BWI76_RS03950: BWI76_RS03950 - sigma-54 interacting domain-containing protein, at 816,725 to 819,490
_RS03950
BWI76_RS03955: BWI76_RS03955 - PTS system fructose subfamily transporter subunit IIA, at 819,590 to 820,012
_RS03955
BWI76_RS03960: BWI76_RS03960 - PTS system sorbose subfamily IIB component, at 820,027 to 820,497
_RS03960
BWI76_RS03965: BWI76_RS03965 - PTS sugar transporter, at 820,514 to 821,293
_RS03965
BWI76_RS03970: BWI76_RS03970 - PTS mannose/fructose/sorbose family transporter subunit IID, at 821,271 to 822,122
_RS03970
Position (kb) 820
821 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 819.088 kb on - strand, within BWI76_RS03950 at 819.090 kb on + strand, within BWI76_RS03950 at 819.090 kb on + strand, within BWI76_RS03950 at 819.098 kb on - strand, within BWI76_RS03950 at 819.098 kb on - strand, within BWI76_RS03950 at 819.272 kb on + strand at 819.275 kb on + strand at 819.285 kb on + strand at 819.374 kb on + strand at 819.425 kb on - strand at 819.513 kb on + strand at 819.526 kb on - strand at 819.638 kb on + strand, within BWI76_RS03955 at 819.638 kb on + strand, within BWI76_RS03955 at 819.638 kb on + strand, within BWI76_RS03955 at 819.640 kb on + strand, within BWI76_RS03955 at 819.640 kb on + strand, within BWI76_RS03955 at 819.645 kb on + strand, within BWI76_RS03955 at 819.646 kb on - strand, within BWI76_RS03955 at 819.646 kb on - strand, within BWI76_RS03955 at 819.646 kb on - strand, within BWI76_RS03955 at 819.646 kb on - strand, within BWI76_RS03955 at 819.648 kb on + strand, within BWI76_RS03955 at 819.648 kb on + strand, within BWI76_RS03955 at 819.648 kb on - strand, within BWI76_RS03955 at 819.648 kb on - strand, within BWI76_RS03955 at 819.648 kb on - strand, within BWI76_RS03955 at 819.648 kb on - strand, within BWI76_RS03955 at 819.648 kb on - strand, within BWI76_RS03955 at 819.653 kb on - strand, within BWI76_RS03955 at 819.673 kb on + strand, within BWI76_RS03955 at 819.681 kb on + strand, within BWI76_RS03955 at 819.681 kb on + strand, within BWI76_RS03955 at 819.689 kb on + strand, within BWI76_RS03955 at 819.689 kb on - strand, within BWI76_RS03955 at 819.689 kb on - strand, within BWI76_RS03955 at 819.689 kb on - strand, within BWI76_RS03955 at 819.689 kb on - strand, within BWI76_RS03955 at 819.699 kb on + strand, within BWI76_RS03955 at 819.699 kb on + strand, within BWI76_RS03955 at 819.702 kb on + strand, within BWI76_RS03955 at 819.707 kb on - strand, within BWI76_RS03955 at 819.710 kb on - strand, within BWI76_RS03955 at 819.711 kb on + strand, within BWI76_RS03955 at 819.715 kb on - strand, within BWI76_RS03955 at 819.719 kb on - strand, within BWI76_RS03955 at 819.730 kb on + strand, within BWI76_RS03955 at 819.730 kb on + strand, within BWI76_RS03955 at 819.730 kb on + strand, within BWI76_RS03955 at 819.730 kb on + strand, within BWI76_RS03955 at 819.730 kb on + strand, within BWI76_RS03955 at 819.738 kb on - strand, within BWI76_RS03955 at 819.738 kb on - strand, within BWI76_RS03955 at 819.738 kb on - strand, within BWI76_RS03955 at 819.740 kb on - strand, within BWI76_RS03955 at 819.742 kb on + strand, within BWI76_RS03955 at 819.750 kb on - strand, within BWI76_RS03955 at 819.770 kb on - strand, within BWI76_RS03955 at 819.776 kb on - strand, within BWI76_RS03955 at 819.780 kb on - strand, within BWI76_RS03955 at 819.816 kb on - strand, within BWI76_RS03955 at 819.823 kb on + strand, within BWI76_RS03955 at 819.858 kb on + strand, within BWI76_RS03955 at 819.858 kb on + strand, within BWI76_RS03955 at 819.865 kb on - strand, within BWI76_RS03955 at 819.865 kb on - strand, within BWI76_RS03955 at 819.866 kb on - strand, within BWI76_RS03955 at 819.899 kb on + strand, within BWI76_RS03955 at 819.907 kb on - strand, within BWI76_RS03955 at 819.907 kb on - strand, within BWI76_RS03955 at 819.910 kb on - strand, within BWI76_RS03955 at 819.910 kb on - strand, within BWI76_RS03955 at 819.942 kb on + strand, within BWI76_RS03955 at 819.947 kb on + strand, within BWI76_RS03955 at 819.954 kb on + strand, within BWI76_RS03955 at 819.956 kb on + strand, within BWI76_RS03955 at 819.969 kb on - strand, within BWI76_RS03955 at 819.969 kb on - strand, within BWI76_RS03955 at 819.983 kb on + strand at 820.006 kb on - strand at 820.014 kb on - strand at 820.030 kb on - strand at 820.038 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.060 kb on + strand at 820.068 kb on - strand at 820.075 kb on + strand, within BWI76_RS03960 at 820.083 kb on - strand, within BWI76_RS03960 at 820.125 kb on + strand at 820.149 kb on + strand, within BWI76_RS03960 at 820.184 kb on + strand, within BWI76_RS03960 at 820.184 kb on + strand, within BWI76_RS03960 at 820.184 kb on + strand, within BWI76_RS03960 at 820.184 kb on + strand, within BWI76_RS03960 at 820.184 kb on + strand, within BWI76_RS03960 at 820.192 kb on - strand, within BWI76_RS03960 at 820.192 kb on - strand, within BWI76_RS03960 at 820.217 kb on + strand, within BWI76_RS03960 at 820.217 kb on + strand, within BWI76_RS03960 at 820.225 kb on - strand, within BWI76_RS03960 at 820.267 kb on + strand, within BWI76_RS03960 at 820.278 kb on + strand, within BWI76_RS03960 at 820.286 kb on - strand, within BWI76_RS03960 at 820.287 kb on + strand, within BWI76_RS03960 at 820.287 kb on + strand, within BWI76_RS03960 at 820.300 kb on - strand, within BWI76_RS03960 at 820.302 kb on - strand, within BWI76_RS03960 at 820.325 kb on + strand, within BWI76_RS03960 at 820.325 kb on + strand, within BWI76_RS03960 at 820.333 kb on - strand, within BWI76_RS03960 at 820.333 kb on - strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.341 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.346 kb on + strand, within BWI76_RS03960 at 820.347 kb on + strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.349 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.354 kb on - strand, within BWI76_RS03960 at 820.356 kb on - strand, within BWI76_RS03960 at 820.408 kb on - strand, within BWI76_RS03960 at 820.411 kb on + strand at 820.418 kb on - strand, within BWI76_RS03960 at 820.422 kb on + strand, within BWI76_RS03960 at 820.430 kb on - strand, within BWI76_RS03960 at 820.432 kb on + strand, within BWI76_RS03960 at 820.433 kb on + strand, within BWI76_RS03960 at 820.439 kb on + strand, within BWI76_RS03960 at 820.440 kb on - strand, within BWI76_RS03960 at 820.441 kb on + strand, within BWI76_RS03960 at 820.441 kb on + strand, within BWI76_RS03960 at 820.441 kb on + strand, within BWI76_RS03960 at 820.441 kb on - strand, within BWI76_RS03960 at 820.447 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.449 kb on - strand, within BWI76_RS03960 at 820.456 kb on + strand at 820.463 kb on - strand at 820.476 kb on + strand at 820.510 kb on + strand at 820.510 kb on + strand at 820.510 kb on + strand at 820.510 kb on + strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.518 kb on - strand at 820.526 kb on + strand at 820.534 kb on - strand at 820.544 kb on - strand at 820.544 kb on - strand at 820.620 kb on + strand, within BWI76_RS03965 at 820.625 kb on + strand, within BWI76_RS03965 at 820.628 kb on - strand, within BWI76_RS03965 at 820.721 kb on - strand, within BWI76_RS03965 at 820.809 kb on - strand, within BWI76_RS03965 at 820.843 kb on + strand, within BWI76_RS03965 at 820.882 kb on - strand, within BWI76_RS03965 at 820.892 kb on - strand, within BWI76_RS03965 at 820.965 kb on - strand, within BWI76_RS03965 at 821.214 kb on + strand, within BWI76_RS03965 at 821.327 kb on - strand at 821.357 kb on + strand, within BWI76_RS03970 at 821.450 kb on + strand, within BWI76_RS03970
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Escherichia coli BW25113 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 819,088 - BWI76_RS03950 0.85 +0.1 819,090 + BWI76_RS03950 0.86 -1.7 819,090 + BWI76_RS03950 0.86 -0.2 819,098 - BWI76_RS03950 0.86 +0.5 819,098 - BWI76_RS03950 0.86 -0.0 819,272 + +1.5 819,275 + +0.4 819,285 + -0.4 819,374 + -0.5 819,425 - +0.1 819,513 + +0.5 819,526 - -0.4 819,638 + BWI76_RS03955 0.11 +0.8 819,638 + BWI76_RS03955 0.11 +0.2 819,638 + BWI76_RS03955 0.11 +0.2 819,640 + BWI76_RS03955 0.12 +0.6 819,640 + BWI76_RS03955 0.12 +0.4 819,645 + BWI76_RS03955 0.13 -1.5 819,646 - BWI76_RS03955 0.13 +0.8 819,646 - BWI76_RS03955 0.13 -1.5 819,646 - BWI76_RS03955 0.13 -0.1 819,646 - BWI76_RS03955 0.13 -2.5 819,648 + BWI76_RS03955 0.14 +0.1 819,648 + BWI76_RS03955 0.14 -0.8 819,648 - BWI76_RS03955 0.14 -0.5 819,648 - BWI76_RS03955 0.14 -0.2 819,648 - BWI76_RS03955 0.14 +1.3 819,648 - BWI76_RS03955 0.14 +0.2 819,648 - BWI76_RS03955 0.14 +0.3 819,653 - BWI76_RS03955 0.15 +1.0 819,673 + BWI76_RS03955 0.20 +0.2 819,681 + BWI76_RS03955 0.22 -0.0 819,681 + BWI76_RS03955 0.22 -0.9 819,689 + BWI76_RS03955 0.23 +0.5 819,689 - BWI76_RS03955 0.23 +0.3 819,689 - BWI76_RS03955 0.23 -0.1 819,689 - BWI76_RS03955 0.23 +0.5 819,689 - BWI76_RS03955 0.23 +0.1 819,699 + BWI76_RS03955 0.26 -0.1 819,699 + BWI76_RS03955 0.26 -0.6 819,702 + BWI76_RS03955 0.26 -0.7 819,707 - BWI76_RS03955 0.28 -1.8 819,710 - BWI76_RS03955 0.28 -0.2 819,711 + BWI76_RS03955 0.29 -0.9 819,715 - BWI76_RS03955 0.30 +0.2 819,719 - BWI76_RS03955 0.30 +0.3 819,730 + BWI76_RS03955 0.33 -0.4 819,730 + BWI76_RS03955 0.33 -0.5 819,730 + BWI76_RS03955 0.33 -0.1 819,730 + BWI76_RS03955 0.33 -1.1 819,730 + BWI76_RS03955 0.33 -0.5 819,738 - BWI76_RS03955 0.35 -0.1 819,738 - BWI76_RS03955 0.35 -0.5 819,738 - BWI76_RS03955 0.35 +0.1 819,740 - BWI76_RS03955 0.35 -0.9 819,742 + BWI76_RS03955 0.36 +0.7 819,750 - BWI76_RS03955 0.38 +0.2 819,770 - BWI76_RS03955 0.43 -0.2 819,776 - BWI76_RS03955 0.44 +0.0 819,780 - BWI76_RS03955 0.45 +0.4 819,816 - BWI76_RS03955 0.53 -0.3 819,823 + BWI76_RS03955 0.55 -0.6 819,858 + BWI76_RS03955 0.63 -1.0 819,858 + BWI76_RS03955 0.63 -0.5 819,865 - BWI76_RS03955 0.65 -1.5 819,865 - BWI76_RS03955 0.65 -0.6 819,866 - BWI76_RS03955 0.65 -0.1 819,899 + BWI76_RS03955 0.73 +0.5 819,907 - BWI76_RS03955 0.75 -1.5 819,907 - BWI76_RS03955 0.75 -0.6 819,910 - BWI76_RS03955 0.76 -0.1 819,910 - BWI76_RS03955 0.76 -0.6 819,942 + BWI76_RS03955 0.83 -0.0 819,947 + BWI76_RS03955 0.84 -0.3 819,954 + BWI76_RS03955 0.86 +0.3 819,956 + BWI76_RS03955 0.87 -0.0 819,969 - BWI76_RS03955 0.90 +0.9 819,969 - BWI76_RS03955 0.90 +0.1 819,983 + -0.2 820,006 - -1.2 820,014 - -1.9 820,030 - +2.7 820,038 + +1.7 820,060 + +2.1 820,060 + -0.2 820,060 + +0.4 820,060 + +1.6 820,060 + +2.7 820,060 + +1.3 820,060 + -1.7 820,060 + -0.5 820,068 - -0.1 820,075 + BWI76_RS03960 0.10 +0.6 820,083 - BWI76_RS03960 0.12 -0.9 820,125 + +0.1 820,149 + BWI76_RS03960 0.26 -0.5 820,184 + BWI76_RS03960 0.33 +0.3 820,184 + BWI76_RS03960 0.33 +1.8 820,184 + BWI76_RS03960 0.33 -1.3 820,184 + BWI76_RS03960 0.33 -2.2 820,184 + BWI76_RS03960 0.33 +0.5 820,192 - BWI76_RS03960 0.35 +0.8 820,192 - BWI76_RS03960 0.35 +0.1 820,217 + BWI76_RS03960 0.40 -0.1 820,217 + BWI76_RS03960 0.40 -1.1 820,225 - BWI76_RS03960 0.42 -0.7 820,267 + BWI76_RS03960 0.51 -0.1 820,278 + BWI76_RS03960 0.53 -1.4 820,286 - BWI76_RS03960 0.55 +0.8 820,287 + BWI76_RS03960 0.55 -0.3 820,287 + BWI76_RS03960 0.55 -0.1 820,300 - BWI76_RS03960 0.58 -0.1 820,302 - BWI76_RS03960 0.58 -0.4 820,325 + BWI76_RS03960 0.63 -0.4 820,325 + BWI76_RS03960 0.63 +0.2 820,333 - BWI76_RS03960 0.65 +1.1 820,333 - BWI76_RS03960 0.65 -0.3 820,341 + BWI76_RS03960 0.67 -1.5 820,341 + BWI76_RS03960 0.67 -0.7 820,341 + BWI76_RS03960 0.67 -0.3 820,341 + BWI76_RS03960 0.67 +0.3 820,341 + BWI76_RS03960 0.67 -1.9 820,341 + BWI76_RS03960 0.67 -0.5 820,346 + BWI76_RS03960 0.68 -0.4 820,346 + BWI76_RS03960 0.68 -0.3 820,346 + BWI76_RS03960 0.68 -0.7 820,346 + BWI76_RS03960 0.68 -0.4 820,346 + BWI76_RS03960 0.68 -0.8 820,346 + BWI76_RS03960 0.68 -0.3 820,346 + BWI76_RS03960 0.68 -1.2 820,346 + BWI76_RS03960 0.68 -0.1 820,346 + BWI76_RS03960 0.68 -0.2 820,346 + BWI76_RS03960 0.68 +0.9 820,346 + BWI76_RS03960 0.68 +0.7 820,347 + BWI76_RS03960 0.68 -0.7 820,349 - BWI76_RS03960 0.68 +0.2 820,349 - BWI76_RS03960 0.68 +0.1 820,349 - BWI76_RS03960 0.68 +1.1 820,349 - BWI76_RS03960 0.68 -0.5 820,349 - BWI76_RS03960 0.68 -1.2 820,349 - BWI76_RS03960 0.68 -0.5 820,354 - BWI76_RS03960 0.69 +0.9 820,354 - BWI76_RS03960 0.69 -0.6 820,354 - BWI76_RS03960 0.69 -0.6 820,354 - BWI76_RS03960 0.69 +1.9 820,354 - BWI76_RS03960 0.69 +1.3 820,354 - BWI76_RS03960 0.69 -0.0 820,356 - BWI76_RS03960 0.70 -0.6 820,408 - BWI76_RS03960 0.81 -0.9 820,411 + -0.9 820,418 - BWI76_RS03960 0.83 -1.4 820,422 + BWI76_RS03960 0.84 -0.4 820,430 - BWI76_RS03960 0.86 +0.4 820,432 + BWI76_RS03960 0.86 -0.3 820,433 + BWI76_RS03960 0.86 -1.7 820,439 + BWI76_RS03960 0.87 -0.5 820,440 - BWI76_RS03960 0.88 -0.5 820,441 + BWI76_RS03960 0.88 -0.4 820,441 + BWI76_RS03960 0.88 -0.0 820,441 + BWI76_RS03960 0.88 -0.0 820,441 - BWI76_RS03960 0.88 +0.0 820,447 - BWI76_RS03960 0.89 +1.9 820,449 - BWI76_RS03960 0.90 -0.9 820,449 - BWI76_RS03960 0.90 -0.9 820,449 - BWI76_RS03960 0.90 +0.1 820,449 - BWI76_RS03960 0.90 +0.0 820,449 - BWI76_RS03960 0.90 -0.3 820,449 - BWI76_RS03960 0.90 +0.1 820,456 + +0.1 820,463 - +1.9 820,476 + +0.0 820,510 + +1.3 820,510 + +0.1 820,510 + -0.9 820,510 + -2.0 820,518 - +0.8 820,518 - -0.0 820,518 - -0.6 820,518 - -1.2 820,518 - +0.8 820,518 - -0.9 820,518 - -0.5 820,518 - -3.0 820,518 - +1.1 820,526 + -0.0 820,534 - -0.3 820,544 - +0.6 820,544 - -2.2 820,620 + BWI76_RS03965 0.14 +0.3 820,625 + BWI76_RS03965 0.14 -0.6 820,628 - BWI76_RS03965 0.15 -1.7 820,721 - BWI76_RS03965 0.27 -1.3 820,809 - BWI76_RS03965 0.38 +0.1 820,843 + BWI76_RS03965 0.42 -1.2 820,882 - BWI76_RS03965 0.47 +0.3 820,892 - BWI76_RS03965 0.48 +0.0 820,965 - BWI76_RS03965 0.58 -2.3 821,214 + BWI76_RS03965 0.90 -1.9 821,327 - -0.4 821,357 + BWI76_RS03970 0.10 +0.5 821,450 + BWI76_RS03970 0.21 -0.9
Or see this region's nucleotide sequence