Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10615

Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10610 and BWI76_RS10615 are separated by 182 nucleotidesBWI76_RS10615 and BWI76_RS10620 are separated by 130 nucleotides BWI76_RS10610: BWI76_RS10610 - helix-turn-helix transcriptional regulator, at 2,241,450 to 2,241,998 _RS10610 BWI76_RS10615: BWI76_RS10615 - chorismate mutase, at 2,242,181 to 2,242,477 _RS10615 BWI76_RS10620: BWI76_RS10620 - hypothetical protein, at 2,242,608 to 2,243,672 _RS10620 Position (kb) 2242 2243Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2241.296 kb on + strandat 2241.314 kb on + strandat 2241.323 kb on + strandat 2241.331 kb on - strandat 2241.331 kb on - strandat 2241.353 kb on + strandat 2241.373 kb on - strandat 2241.379 kb on + strandat 2241.402 kb on + strandat 2241.435 kb on + strandat 2241.435 kb on + strandat 2241.525 kb on + strandat 2241.525 kb on + strand, within BWI76_RS10610at 2241.525 kb on + strand, within BWI76_RS10610at 2241.531 kb on + strand, within BWI76_RS10610at 2241.531 kb on + strand, within BWI76_RS10610at 2241.531 kb on + strand, within BWI76_RS10610at 2241.578 kb on + strand, within BWI76_RS10610at 2241.586 kb on - strand, within BWI76_RS10610at 2241.611 kb on + strand, within BWI76_RS10610at 2241.616 kb on + strand, within BWI76_RS10610at 2241.725 kb on + strand, within BWI76_RS10610at 2241.892 kb on + strand, within BWI76_RS10610at 2242.116 kb on + strandat 2242.214 kb on - strand, within BWI76_RS10615at 2242.302 kb on + strand, within BWI76_RS10615at 2242.308 kb on - strand, within BWI76_RS10615at 2242.408 kb on + strand, within BWI76_RS10615at 2242.408 kb on - strand, within BWI76_RS10615at 2242.422 kb on + strandat 2242.422 kb on + strand, within BWI76_RS10615at 2242.430 kb on - strand, within BWI76_RS10615at 2242.452 kb on + strandat 2242.545 kb on + strandat 2242.567 kb on - strandat 2242.662 kb on + strandat 2242.707 kb on + strandat 2242.760 kb on + strand, within BWI76_RS10620at 2242.796 kb on + strand, within BWI76_RS10620at 2242.888 kb on + strand, within BWI76_RS10620at 2242.922 kb on - strand, within BWI76_RS10620at 2242.938 kb on + strand, within BWI76_RS10620at 2242.943 kb on + strand, within BWI76_RS10620at 2242.949 kb on - strand, within BWI76_RS10620at 2242.951 kb on - strand, within BWI76_RS10620at 2243.086 kb on + strandat 2243.094 kb on - strand, within BWI76_RS10620at 2243.133 kb on - strand, within BWI76_RS10620at 2243.168 kb on - strand, within BWI76_RS10620at 2243.194 kb on + strand, within BWI76_RS10620at 2243.217 kb on - strand, within BWI76_RS10620at 2243.449 kb on + strand, within BWI76_RS10620at 2243.450 kb on + strand, within BWI76_RS10620at 2243.464 kb on + strand, within BWI76_RS10620at 2243.472 kb on + strand, within BWI76_RS10620at 2243.472 kb on - strand, within BWI76_RS10620

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,241,296 + +0.5
2,241,314 + +0.8
2,241,323 + -0.9
2,241,331 - -0.3
2,241,331 - -2.1
2,241,353 + -0.9
2,241,373 - +0.5
2,241,379 + -2.6
2,241,402 + +1.1
2,241,435 + -0.2
2,241,435 + -0.2
2,241,525 + +0.7
2,241,525 + BWI76_RS10610 0.14 -1.1
2,241,525 + BWI76_RS10610 0.14 -0.5
2,241,531 + BWI76_RS10610 0.15 -2.8
2,241,531 + BWI76_RS10610 0.15 -0.2
2,241,531 + BWI76_RS10610 0.15 -0.1
2,241,578 + BWI76_RS10610 0.23 -0.4
2,241,586 - BWI76_RS10610 0.25 -0.3
2,241,611 + BWI76_RS10610 0.29 +0.9
2,241,616 + BWI76_RS10610 0.30 -0.2
2,241,725 + BWI76_RS10610 0.50 +0.6
2,241,892 + BWI76_RS10610 0.81 +0.7
2,242,116 + -1.4
2,242,214 - BWI76_RS10615 0.11 +1.1
2,242,302 + BWI76_RS10615 0.41 -0.8
2,242,308 - BWI76_RS10615 0.43 -0.1
2,242,408 + BWI76_RS10615 0.76 -0.5
2,242,408 - BWI76_RS10615 0.76 +1.4
2,242,422 + -1.9
2,242,422 + BWI76_RS10615 0.81 +0.3
2,242,430 - BWI76_RS10615 0.84 +0.6
2,242,452 + +0.3
2,242,545 + +0.7
2,242,567 - +0.4
2,242,662 + +0.7
2,242,707 + +0.1
2,242,760 + BWI76_RS10620 0.14 -0.1
2,242,796 + BWI76_RS10620 0.18 -0.0
2,242,888 + BWI76_RS10620 0.26 +0.6
2,242,922 - BWI76_RS10620 0.29 -0.7
2,242,938 + BWI76_RS10620 0.31 -1.5
2,242,943 + BWI76_RS10620 0.31 -0.3
2,242,949 - BWI76_RS10620 0.32 -0.3
2,242,951 - BWI76_RS10620 0.32 +0.7
2,243,086 + +0.7
2,243,094 - BWI76_RS10620 0.46 -0.0
2,243,133 - BWI76_RS10620 0.49 -0.3
2,243,168 - BWI76_RS10620 0.53 +0.9
2,243,194 + BWI76_RS10620 0.55 -0.3
2,243,217 - BWI76_RS10620 0.57 -1.1
2,243,449 + BWI76_RS10620 0.79 -0.7
2,243,450 + BWI76_RS10620 0.79 +2.1
2,243,464 + BWI76_RS10620 0.80 +0.6
2,243,472 + BWI76_RS10620 0.81 +0.2
2,243,472 - BWI76_RS10620 0.81 -0.3

Or see this region's nucleotide sequence