Experiment: RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00950 and BWI76_RS00955 are separated by 349 nucleotides BWI76_RS00955 and BWI76_RS00960 are separated by 139 nucleotides BWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotides BWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides
BWI76_RS00950: BWI76_RS00950 - ATP-dependent protease, at 193,279 to 194,799
_RS00950
BWI76_RS00955: BWI76_RS00955 - IlvGMEDA operon leader peptide, at 195,149 to 195,247
_RS00955
BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033
_RS00960
BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287
_RS00965
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 194.428 kb on + strand, within BWI76_RS00950 at 194.517 kb on + strand, within BWI76_RS00950 at 194.525 kb on - strand, within BWI76_RS00950 at 194.656 kb on - strand at 194.699 kb on - strand at 194.748 kb on + strand at 194.769 kb on - strand at 194.784 kb on - strand at 194.948 kb on + strand at 195.501 kb on + strand at 195.528 kb on - strand at 195.528 kb on - strand at 195.565 kb on - strand, within BWI76_RS00960 at 195.595 kb on + strand, within BWI76_RS00960 at 195.642 kb on - strand at 195.652 kb on - strand, within BWI76_RS00960 at 195.841 kb on - strand, within BWI76_RS00960 at 196.023 kb on + strand, within BWI76_RS00960 at 196.149 kb on - strand, within BWI76_RS00960 at 196.149 kb on - strand, within BWI76_RS00960 at 196.541 kb on - strand, within BWI76_RS00960 at 196.597 kb on + strand at 196.605 kb on - strand, within BWI76_RS00960 at 196.754 kb on + strand, within BWI76_RS00960 at 196.869 kb on + strand at 196.933 kb on + strand at 196.941 kb on - strand at 196.973 kb on + strand at 196.981 kb on - strand at 197.236 kb on - strand, within BWI76_RS00965 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Cupriavidus sp. AnTP704 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 194,428 + BWI76_RS00950 0.76 -1.6 194,517 + BWI76_RS00950 0.81 -0.5 194,525 - BWI76_RS00950 0.82 -0.5 194,656 - +0.3 194,699 - +0.2 194,748 + -0.1 194,769 - +0.5 194,784 - +0.3 194,948 + -0.2 195,501 + -1.5 195,528 - -2.0 195,528 - -1.8 195,565 - BWI76_RS00960 0.11 -2.9 195,595 + BWI76_RS00960 0.13 -1.4 195,642 - +0.0 195,652 - BWI76_RS00960 0.16 -0.8 195,841 - BWI76_RS00960 0.28 -1.3 196,023 + BWI76_RS00960 0.39 -1.5 196,149 - BWI76_RS00960 0.46 -1.4 196,149 - BWI76_RS00960 0.46 -1.3 196,541 - BWI76_RS00960 0.70 -3.5 196,597 + +1.0 196,605 - BWI76_RS00960 0.74 -2.8 196,754 + BWI76_RS00960 0.83 +0.3 196,869 + -0.0 196,933 + +0.4 196,941 - -1.6 196,973 + +0.4 196,981 - -0.9 197,236 - BWI76_RS00965 0.80 -2.6 197,330 - -1.0 197,383 + -2.3 197,399 - BWI76_RS00970 0.10 -4.7 197,484 + BWI76_RS00970 0.19 -3.5 197,484 + BWI76_RS00970 0.19 -0.6 197,540 + BWI76_RS00970 0.25 -0.7 197,540 + BWI76_RS00970 0.25 +0.0 197,686 + BWI76_RS00970 0.41 -0.2
Or see this region's nucleotide sequence