Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23700 and BWI76_RS23705 are separated by 2 nucleotides BWI76_RS23705 and BWI76_RS23710 are separated by 11 nucleotides BWI76_RS23710 and BWI76_RS23715 are separated by 135 nucleotides
BWI76_RS23700: BWI76_RS23700 - glycerate CoA-transferase (from data) , at 4,946,698 to 4,948,299
_RS23700
BWI76_RS23705: BWI76_RS23705 - 2-deoxy-D-ribonyl-CoA 3-dehydrogenase (from data) , at 4,948,302 to 4,949,036
_RS23705
BWI76_RS23710: BWI76_RS23710 - 3-keto-2-deoxy-D-ribonyl-CoA thiolase (from data) , at 4,949,048 to 4,950,238
_RS23710
BWI76_RS23715: BWI76_RS23715 - 2-deoxy-D-ribonate transporter 1 (from data) , at 4,950,374 to 4,951,711
_RS23715
Position (kb)
4949
4950
4951 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4948.120 kb on + strand, within BWI76_RS23700 at 4948.368 kb on - strand at 4948.502 kb on + strand, within BWI76_RS23705 at 4948.657 kb on + strand, within BWI76_RS23705 at 4949.140 kb on + strand at 4949.173 kb on - strand, within BWI76_RS23710 at 4949.429 kb on - strand, within BWI76_RS23710 at 4949.480 kb on + strand, within BWI76_RS23710 at 4949.754 kb on - strand, within BWI76_RS23710 at 4949.876 kb on + strand, within BWI76_RS23710 at 4950.029 kb on + strand, within BWI76_RS23710 at 4950.593 kb on + strand, within BWI76_RS23715 at 4950.593 kb on + strand, within BWI76_RS23715 at 4950.601 kb on - strand, within BWI76_RS23715 at 4950.601 kb on - strand, within BWI76_RS23715 at 4950.640 kb on + strand, within BWI76_RS23715 at 4950.640 kb on + strand, within BWI76_RS23715 at 4950.645 kb on + strand, within BWI76_RS23715 at 4951.044 kb on - strand, within BWI76_RS23715 at 4951.165 kb on - strand, within BWI76_RS23715
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,948,120 + BWI76_RS23700 0.89 -0.3 4,948,368 - +0.9 4,948,502 + BWI76_RS23705 0.27 -0.4 4,948,657 + BWI76_RS23705 0.48 +0.2 4,949,140 + +1.0 4,949,173 - BWI76_RS23710 0.10 -0.0 4,949,429 - BWI76_RS23710 0.32 +1.3 4,949,480 + BWI76_RS23710 0.36 -0.1 4,949,754 - BWI76_RS23710 0.59 +1.9 4,949,876 + BWI76_RS23710 0.70 -0.8 4,950,029 + BWI76_RS23710 0.82 -0.1 4,950,593 + BWI76_RS23715 0.16 -1.4 4,950,593 + BWI76_RS23715 0.16 -0.3 4,950,601 - BWI76_RS23715 0.17 -0.3 4,950,601 - BWI76_RS23715 0.17 +0.1 4,950,640 + BWI76_RS23715 0.20 +1.0 4,950,640 + BWI76_RS23715 0.20 +0.2 4,950,645 + BWI76_RS23715 0.20 -2.4 4,951,044 - BWI76_RS23715 0.50 -0.6 4,951,165 - BWI76_RS23715 0.59 -2.5
Or see this region's nucleotide sequence