Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21465 and BWI76_RS21470 overlap by 4 nucleotides BWI76_RS21470 and BWI76_RS21475 are separated by 68 nucleotides
BWI76_RS21465: BWI76_RS21465 - bifunctional chorismate mutase/prephenate dehydratase, at 4,496,591 to 4,497,751
_RS21465
BWI76_RS21470: BWI76_RS21470 - gluconolaconase, at 4,497,748 to 4,498,620
_RS21470
BWI76_RS21475: BWI76_RS21475 - bifunctional chorismate mutase/prephenate dehydrogenase, at 4,498,689 to 4,499,810
_RS21475
Position (kb)
4497
4498
4499 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4497.319 kb on + strand, within BWI76_RS21465 at 4497.341 kb on + strand, within BWI76_RS21465 at 4497.349 kb on - strand, within BWI76_RS21465 at 4497.354 kb on - strand, within BWI76_RS21465 at 4497.634 kb on - strand, within BWI76_RS21465 at 4497.644 kb on - strand at 4497.655 kb on + strand at 4497.709 kb on - strand at 4497.709 kb on - strand at 4497.888 kb on - strand, within BWI76_RS21470 at 4498.016 kb on + strand, within BWI76_RS21470 at 4498.440 kb on + strand, within BWI76_RS21470 at 4498.521 kb on + strand, within BWI76_RS21470 at 4498.563 kb on + strand at 4498.730 kb on - strand at 4498.908 kb on - strand, within BWI76_RS21475 at 4499.099 kb on - strand, within BWI76_RS21475 at 4499.118 kb on - strand, within BWI76_RS21475 at 4499.220 kb on + strand, within BWI76_RS21475 at 4499.591 kb on - strand, within BWI76_RS21475
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,497,319 + BWI76_RS21465 0.63 -3.2 4,497,341 + BWI76_RS21465 0.65 -2.1 4,497,349 - BWI76_RS21465 0.65 -3.3 4,497,354 - BWI76_RS21465 0.66 -3.9 4,497,634 - BWI76_RS21465 0.90 -4.4 4,497,644 - -0.9 4,497,655 + -2.3 4,497,709 - -0.8 4,497,709 - -1.6 4,497,888 - BWI76_RS21470 0.16 -0.3 4,498,016 + BWI76_RS21470 0.31 -0.3 4,498,440 + BWI76_RS21470 0.79 -1.2 4,498,521 + BWI76_RS21470 0.89 +0.1 4,498,563 + -0.9 4,498,730 - -1.9 4,498,908 - BWI76_RS21475 0.20 -1.8 4,499,099 - BWI76_RS21475 0.37 +0.1 4,499,118 - BWI76_RS21475 0.38 -4.4 4,499,220 + BWI76_RS21475 0.47 -0.9 4,499,591 - BWI76_RS21475 0.80 -1.5
Or see this region's nucleotide sequence