Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18075 and BWI76_RS18080 are separated by 133 nucleotides BWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotides BWI76_RS18085 and BWI76_RS18090 are separated by 135 nucleotides
BWI76_RS18075: BWI76_RS18075 - oligopeptidase B, at 3,754,435 to 3,756,495
_RS18075
BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309
_RS18080
BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816
_RS18085
BWI76_RS18090: BWI76_RS18090 - phosphoribosylglycinamide formyltransferase 2, at 3,757,952 to 3,759,130
_RS18090
Position (kb)
3756
3757
3758 Strain fitness (log2 ratio)
-2
-1
0
1 at 3755.856 kb on + strand, within BWI76_RS18075 at 3756.276 kb on - strand, within BWI76_RS18075 at 3756.525 kb on - strand at 3756.544 kb on + strand at 3756.562 kb on - strand at 3756.570 kb on - strand at 3756.626 kb on + strand at 3756.824 kb on + strand, within BWI76_RS18080 at 3757.080 kb on + strand, within BWI76_RS18080 at 3757.534 kb on - strand, within BWI76_RS18085 at 3757.869 kb on + strand at 3757.992 kb on + strand at 3758.028 kb on - strand at 3758.214 kb on + strand, within BWI76_RS18090
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,755,856 + BWI76_RS18075 0.69 +0.4 3,756,276 - BWI76_RS18075 0.89 -1.0 3,756,525 - +1.0 3,756,544 + +0.8 3,756,562 - -0.3 3,756,570 - -1.0 3,756,626 + -0.0 3,756,824 + BWI76_RS18080 0.29 +0.7 3,757,080 + BWI76_RS18080 0.66 +0.9 3,757,534 - BWI76_RS18085 0.22 -1.7 3,757,869 + -0.7 3,757,992 + -1.1 3,758,028 - -0.5 3,758,214 + BWI76_RS18090 0.22 -1.9
Or see this region's nucleotide sequence