Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17105 and BWI76_RS17110 are separated by 24 nucleotides BWI76_RS17110 and BWI76_RS17115 are separated by 96 nucleotides BWI76_RS17115 and BWI76_RS17120 are separated by 4 nucleotides
BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635
_RS17105
BWI76_RS17110: BWI76_RS17110 - vitamin B12 ABC transporter permease BtuC, at 3,549,660 to 3,550,646
_RS17110
BWI76_RS17115: BWI76_RS17115 - integration host factor subunit alpha, at 3,550,743 to 3,551,042
_RS17115
BWI76_RS17120: BWI76_RS17120 - phenylalanine--tRNA ligase subunit beta, at 3,551,047 to 3,553,434
_RS17120
Position (kb)
3549
3550
3551 Strain fitness (log2 ratio)
-2
-1
0
1 at 3548.743 kb on - strand at 3548.856 kb on + strand at 3548.868 kb on + strand at 3548.882 kb on + strand at 3548.890 kb on - strand at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.091 kb on + strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.137 kb on + strand, within BWI76_RS17105 at 3549.375 kb on + strand, within BWI76_RS17105 at 3549.378 kb on - strand, within BWI76_RS17105 at 3549.382 kb on + strand, within BWI76_RS17105 at 3549.383 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.441 kb on - strand at 3549.446 kb on - strand, within BWI76_RS17105 at 3549.492 kb on - strand, within BWI76_RS17105 at 3549.613 kb on - strand at 3549.713 kb on + strand at 3550.305 kb on + strand, within BWI76_RS17110 at 3550.595 kb on - strand at 3550.638 kb on + strand at 3551.047 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 3,548,743 - -0.2 3,548,856 + -0.2 3,548,868 + -0.7 3,548,882 + -1.2 3,548,890 - -2.5 3,549,086 + BWI76_RS17105 0.31 -0.9 3,549,086 + BWI76_RS17105 0.31 +0.4 3,549,091 + BWI76_RS17105 0.32 -0.3 3,549,094 - BWI76_RS17105 0.32 -0.2 3,549,094 - BWI76_RS17105 0.32 -2.5 3,549,137 + BWI76_RS17105 0.38 -2.3 3,549,375 + BWI76_RS17105 0.67 +0.1 3,549,378 - BWI76_RS17105 0.68 +0.6 3,549,382 + BWI76_RS17105 0.68 -0.5 3,549,383 - BWI76_RS17105 0.68 -0.6 3,549,390 - BWI76_RS17105 0.69 -0.1 3,549,390 - BWI76_RS17105 0.69 +0.0 3,549,441 - +0.7 3,549,446 - BWI76_RS17105 0.76 -0.3 3,549,492 - BWI76_RS17105 0.82 +0.1 3,549,613 - +1.2 3,549,713 + -1.8 3,550,305 + BWI76_RS17110 0.65 -0.5 3,550,595 - -1.5 3,550,638 + +0.5 3,551,047 - -0.2
Or see this region's nucleotide sequence