Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14850

Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14845 and BWI76_RS14850 are separated by 366 nucleotidesBWI76_RS14850 and BWI76_RS14855 are separated by 255 nucleotides BWI76_RS14845: BWI76_RS14845 - hypothetical protein, at 3,109,422 to 3,110,048 _RS14845 BWI76_RS14850: BWI76_RS14850 - LacI family transcriptional regulator, at 3,110,415 to 3,111,446 _RS14850 BWI76_RS14855: BWI76_RS14855 - periplasmic binding protein/LacI transcriptional regulator, at 3,111,702 to 3,112,655 _RS14855 Position (kb) 3110 3111 3112Strain fitness (log2 ratio) -3 -2 -1 0 1at 3109.674 kb on - strand, within BWI76_RS14845at 3109.679 kb on - strand, within BWI76_RS14845at 3109.803 kb on - strand, within BWI76_RS14845at 3110.173 kb on - strandat 3110.219 kb on + strandat 3110.219 kb on + strandat 3110.219 kb on + strandat 3110.826 kb on - strand, within BWI76_RS14850at 3110.991 kb on - strand, within BWI76_RS14850at 3111.013 kb on - strand, within BWI76_RS14850at 3111.266 kb on + strand, within BWI76_RS14850at 3111.274 kb on - strand, within BWI76_RS14850at 3111.330 kb on + strand, within BWI76_RS14850at 3111.372 kb on - strandat 3111.468 kb on + strandat 3111.567 kb on + strandat 3111.651 kb on + strandat 3111.652 kb on + strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.660 kb on - strandat 3111.842 kb on + strand, within BWI76_RS14855at 3111.844 kb on - strand, within BWI76_RS14855at 3111.907 kb on + strand, within BWI76_RS14855at 3111.972 kb on - strand, within BWI76_RS14855at 3112.231 kb on - strand, within BWI76_RS14855at 3112.435 kb on + strand, within BWI76_RS14855

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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3,109,674 - BWI76_RS14845 0.40 +1.6
3,109,679 - BWI76_RS14845 0.41 +0.2
3,109,803 - BWI76_RS14845 0.61 -0.3
3,110,173 - +0.3
3,110,219 + +0.4
3,110,219 + +1.2
3,110,219 + -0.1
3,110,826 - BWI76_RS14850 0.40 -3.2
3,110,991 - BWI76_RS14850 0.56 -0.3
3,111,013 - BWI76_RS14850 0.58 -2.5
3,111,266 + BWI76_RS14850 0.82 -1.0
3,111,274 - BWI76_RS14850 0.83 -3.7
3,111,330 + BWI76_RS14850 0.89 +0.0
3,111,372 - -0.5
3,111,468 + -2.8
3,111,567 + -0.8
3,111,651 + +0.1
3,111,652 + +1.0
3,111,660 - +0.3
3,111,660 - -3.5
3,111,660 - +0.4
3,111,660 - -1.0
3,111,842 + BWI76_RS14855 0.15 +0.5
3,111,844 - BWI76_RS14855 0.15 +0.5
3,111,907 + BWI76_RS14855 0.21 +0.9
3,111,972 - BWI76_RS14855 0.28 +1.7
3,112,231 - BWI76_RS14855 0.55 +0.6
3,112,435 + BWI76_RS14855 0.77 -0.1

Or see this region's nucleotide sequence