Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09395 and BWI76_RS09400 are separated by 76 nucleotides BWI76_RS09400 and BWI76_RS09405 are separated by 305 nucleotides BWI76_RS09405 and BWI76_RS09410 overlap by 38 nucleotides
BWI76_RS09395: BWI76_RS09395 - late control gene D protein, at 1,970,770 to 1,971,855
_RS09395
BWI76_RS09400: BWI76_RS09400 - phage transcriptional activator, Ogr/delta, at 1,971,932 to 1,972,147
_RS09400
BWI76_RS09405: BWI76_RS09405 - hypothetical protein, at 1,972,453 to 1,972,632
_RS09405
BWI76_RS09410: BWI76_RS09410 - reverse transcriptase, at 1,972,595 to 1,973,191
_RS09410
Position (kb) 1971
1972
1973 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1971.182 kb on + strand, within BWI76_RS09395 at 1971.190 kb on - strand, within BWI76_RS09395 at 1971.309 kb on + strand, within BWI76_RS09395 at 1971.311 kb on + strand, within BWI76_RS09395 at 1971.574 kb on - strand, within BWI76_RS09395 at 1971.617 kb on - strand at 1971.657 kb on + strand, within BWI76_RS09395 at 1971.684 kb on + strand, within BWI76_RS09395 at 1971.713 kb on - strand, within BWI76_RS09395 at 1971.745 kb on - strand, within BWI76_RS09395 at 1971.755 kb on - strand at 1971.791 kb on - strand at 1971.915 kb on + strand at 1972.037 kb on + strand, within BWI76_RS09400 at 1972.070 kb on - strand, within BWI76_RS09400 at 1972.135 kb on - strand at 1972.188 kb on + strand at 1972.234 kb on + strand at 1972.249 kb on + strand at 1972.293 kb on - strand at 1972.329 kb on + strand at 1972.331 kb on + strand at 1972.434 kb on - strand at 1972.434 kb on - strand at 1972.434 kb on - strand at 1972.441 kb on + strand at 1972.458 kb on - strand at 1972.477 kb on + strand, within BWI76_RS09405 at 1972.477 kb on + strand, within BWI76_RS09405 at 1972.509 kb on + strand, within BWI76_RS09405 at 1972.509 kb on + strand, within BWI76_RS09405 at 1972.534 kb on + strand, within BWI76_RS09405 at 1972.544 kb on + strand, within BWI76_RS09405 at 1972.570 kb on + strand, within BWI76_RS09405 at 1972.638 kb on + strand at 1972.646 kb on + strand at 1972.646 kb on - strand at 1972.657 kb on + strand, within BWI76_RS09410 at 1972.665 kb on + strand, within BWI76_RS09410 at 1972.665 kb on + strand, within BWI76_RS09410 at 1972.673 kb on - strand, within BWI76_RS09410 at 1972.691 kb on + strand, within BWI76_RS09410 at 1972.770 kb on - strand, within BWI76_RS09410 at 1972.881 kb on + strand, within BWI76_RS09410 at 1972.974 kb on + strand, within BWI76_RS09410 at 1972.974 kb on + strand, within BWI76_RS09410 at 1973.007 kb on - strand, within BWI76_RS09410 at 1973.019 kb on + strand, within BWI76_RS09410 at 1973.049 kb on + strand, within BWI76_RS09410 at 1973.107 kb on + strand, within BWI76_RS09410
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 1,971,182 + BWI76_RS09395 0.38 +0.4 1,971,190 - BWI76_RS09395 0.39 +1.0 1,971,309 + BWI76_RS09395 0.50 -0.1 1,971,311 + BWI76_RS09395 0.50 +0.2 1,971,574 - BWI76_RS09395 0.74 -0.3 1,971,617 - -0.6 1,971,657 + BWI76_RS09395 0.82 -0.7 1,971,684 + BWI76_RS09395 0.84 +1.2 1,971,713 - BWI76_RS09395 0.87 -1.7 1,971,745 - BWI76_RS09395 0.90 -1.8 1,971,755 - -0.6 1,971,791 - -0.0 1,971,915 + +0.7 1,972,037 + BWI76_RS09400 0.49 +0.3 1,972,070 - BWI76_RS09400 0.64 -1.1 1,972,135 - -2.2 1,972,188 + -2.8 1,972,234 + -1.3 1,972,249 + -0.2 1,972,293 - -1.6 1,972,329 + -0.9 1,972,331 + +0.4 1,972,434 - -2.0 1,972,434 - -1.3 1,972,434 - -3.5 1,972,441 + -1.4 1,972,458 - -1.3 1,972,477 + BWI76_RS09405 0.13 -0.4 1,972,477 + BWI76_RS09405 0.13 -0.0 1,972,509 + BWI76_RS09405 0.31 -1.0 1,972,509 + BWI76_RS09405 0.31 +0.3 1,972,534 + BWI76_RS09405 0.45 -1.0 1,972,544 + BWI76_RS09405 0.51 -0.0 1,972,570 + BWI76_RS09405 0.65 +1.8 1,972,638 + -0.1 1,972,646 + +0.2 1,972,646 - -1.8 1,972,657 + BWI76_RS09410 0.10 -1.3 1,972,665 + BWI76_RS09410 0.12 +1.0 1,972,665 + BWI76_RS09410 0.12 +0.2 1,972,673 - BWI76_RS09410 0.13 -1.3 1,972,691 + BWI76_RS09410 0.16 -0.6 1,972,770 - BWI76_RS09410 0.29 -1.3 1,972,881 + BWI76_RS09410 0.48 -0.7 1,972,974 + BWI76_RS09410 0.63 +1.0 1,972,974 + BWI76_RS09410 0.63 -0.6 1,973,007 - BWI76_RS09410 0.69 -2.5 1,973,019 + BWI76_RS09410 0.71 -1.3 1,973,049 + BWI76_RS09410 0.76 -1.0 1,973,107 + BWI76_RS09410 0.86 -1.0
Or see this region's nucleotide sequence