Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04620 and BWI76_RS04625 are separated by 87 nucleotides BWI76_RS04625 and BWI76_RS04630 are separated by 231 nucleotides BWI76_RS04630 and BWI76_RS04635 are separated by 46 nucleotides
BWI76_RS04620: BWI76_RS04620 - transcriptional regulator SgrR, at 961,923 to 963,578
_RS04620
BWI76_RS04625: BWI76_RS04625 - glucose uptake inhibitor SgrT, at 963,666 to 963,818
_RS04625
BWI76_RS04630: BWI76_RS04630 - sugar efflux transporter SetB, at 964,050 to 965,234
_RS04630
BWI76_RS04635: BWI76_RS04635 - MFS transporter, at 965,281 to 966,591
_RS04635
Position (kb) 964
965
966 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 963.194 kb on + strand, within BWI76_RS04620 at 963.230 kb on + strand, within BWI76_RS04620 at 963.364 kb on + strand, within BWI76_RS04620 at 963.364 kb on + strand, within BWI76_RS04620 at 963.397 kb on + strand, within BWI76_RS04620 at 963.774 kb on + strand, within BWI76_RS04625 at 963.785 kb on + strand, within BWI76_RS04625 at 963.805 kb on + strand at 963.805 kb on + strand at 963.813 kb on - strand at 963.827 kb on + strand at 963.827 kb on + strand at 963.827 kb on + strand at 963.975 kb on + strand at 963.975 kb on + strand at 963.975 kb on + strand at 964.159 kb on + strand at 964.203 kb on + strand, within BWI76_RS04630 at 964.208 kb on + strand, within BWI76_RS04630 at 964.246 kb on + strand, within BWI76_RS04630 at 964.263 kb on + strand, within BWI76_RS04630 at 964.365 kb on - strand, within BWI76_RS04630 at 964.365 kb on - strand, within BWI76_RS04630 at 964.386 kb on - strand, within BWI76_RS04630 at 964.386 kb on - strand, within BWI76_RS04630 at 964.464 kb on + strand, within BWI76_RS04630 at 964.496 kb on + strand, within BWI76_RS04630 at 964.524 kb on + strand, within BWI76_RS04630 at 964.593 kb on + strand, within BWI76_RS04630 at 964.595 kb on + strand, within BWI76_RS04630 at 964.600 kb on + strand, within BWI76_RS04630 at 964.658 kb on - strand, within BWI76_RS04630 at 964.807 kb on + strand, within BWI76_RS04630 at 964.900 kb on + strand, within BWI76_RS04630 at 964.942 kb on - strand, within BWI76_RS04630 at 964.953 kb on - strand, within BWI76_RS04630 at 964.966 kb on + strand, within BWI76_RS04630 at 964.966 kb on + strand, within BWI76_RS04630 at 964.991 kb on + strand, within BWI76_RS04630 at 964.999 kb on - strand, within BWI76_RS04630 at 964.999 kb on - strand, within BWI76_RS04630 at 965.063 kb on + strand, within BWI76_RS04630 at 965.071 kb on - strand, within BWI76_RS04630 at 965.122 kb on + strand at 965.125 kb on - strand at 965.125 kb on - strand at 965.126 kb on + strand at 965.141 kb on + strand at 965.141 kb on + strand at 965.149 kb on + strand at 965.149 kb on + strand at 965.149 kb on - strand at 965.150 kb on - strand at 965.157 kb on - strand at 965.159 kb on + strand at 965.159 kb on + strand at 965.159 kb on + strand at 965.167 kb on - strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.204 kb on + strand at 965.212 kb on - strand at 965.212 kb on - strand at 965.212 kb on - strand at 965.212 kb on - strand at 965.229 kb on + strand at 965.251 kb on + strand at 965.260 kb on + strand at 965.266 kb on - strand at 965.267 kb on - strand at 965.269 kb on + strand at 965.272 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.277 kb on - strand at 965.287 kb on - strand at 965.449 kb on + strand, within BWI76_RS04635 at 965.522 kb on + strand, within BWI76_RS04635 at 965.534 kb on - strand, within BWI76_RS04635 at 965.570 kb on + strand, within BWI76_RS04635 at 965.570 kb on + strand, within BWI76_RS04635 at 965.570 kb on + strand at 965.578 kb on - strand, within BWI76_RS04635 at 965.580 kb on + strand at 965.595 kb on + strand, within BWI76_RS04635 at 965.595 kb on + strand, within BWI76_RS04635 at 965.595 kb on + strand, within BWI76_RS04635 at 965.595 kb on + strand, within BWI76_RS04635 at 965.595 kb on + strand at 965.595 kb on + strand, within BWI76_RS04635 at 965.595 kb on + strand, within BWI76_RS04635 at 965.603 kb on - strand, within BWI76_RS04635 at 965.603 kb on - strand at 965.603 kb on - strand, within BWI76_RS04635 at 965.603 kb on - strand, within BWI76_RS04635 at 965.603 kb on - strand, within BWI76_RS04635 at 965.603 kb on - strand, within BWI76_RS04635 at 965.603 kb on - strand, within BWI76_RS04635 at 965.613 kb on - strand, within BWI76_RS04635 at 965.728 kb on - strand, within BWI76_RS04635 at 965.728 kb on - strand, within BWI76_RS04635 at 965.730 kb on + strand, within BWI76_RS04635 at 965.736 kb on - strand at 965.738 kb on - strand, within BWI76_RS04635 at 965.738 kb on - strand, within BWI76_RS04635 at 965.751 kb on + strand, within BWI76_RS04635 at 965.755 kb on - strand, within BWI76_RS04635 at 965.789 kb on + strand, within BWI76_RS04635 at 965.797 kb on - strand, within BWI76_RS04635 at 965.797 kb on - strand, within BWI76_RS04635 at 965.797 kb on - strand, within BWI76_RS04635 at 965.804 kb on + strand, within BWI76_RS04635 at 965.807 kb on + strand, within BWI76_RS04635 at 965.815 kb on - strand, within BWI76_RS04635 at 965.816 kb on + strand, within BWI76_RS04635 at 965.817 kb on - strand, within BWI76_RS04635 at 965.846 kb on - strand, within BWI76_RS04635 at 965.846 kb on - strand, within BWI76_RS04635 at 965.862 kb on + strand, within BWI76_RS04635 at 965.869 kb on + strand, within BWI76_RS04635 at 965.872 kb on + strand, within BWI76_RS04635 at 965.872 kb on + strand at 965.872 kb on + strand, within BWI76_RS04635 at 965.873 kb on + strand, within BWI76_RS04635 at 965.877 kb on - strand, within BWI76_RS04635 at 965.881 kb on + strand, within BWI76_RS04635 at 965.881 kb on + strand, within BWI76_RS04635 at 965.881 kb on + strand, within BWI76_RS04635 at 965.889 kb on - strand, within BWI76_RS04635 at 965.889 kb on - strand, within BWI76_RS04635 at 965.896 kb on + strand, within BWI76_RS04635 at 965.904 kb on - strand, within BWI76_RS04635 at 965.904 kb on - strand, within BWI76_RS04635 at 965.904 kb on - strand, within BWI76_RS04635 at 965.904 kb on - strand, within BWI76_RS04635 at 965.947 kb on - strand, within BWI76_RS04635 at 965.955 kb on - strand, within BWI76_RS04635 at 965.955 kb on - strand, within BWI76_RS04635 at 965.990 kb on + strand, within BWI76_RS04635 at 966.069 kb on - strand, within BWI76_RS04635 at 966.108 kb on + strand, within BWI76_RS04635 at 966.108 kb on + strand, within BWI76_RS04635 at 966.108 kb on + strand, within BWI76_RS04635 at 966.108 kb on + strand, within BWI76_RS04635 at 966.116 kb on - strand, within BWI76_RS04635 at 966.126 kb on + strand, within BWI76_RS04635 at 966.136 kb on - strand, within BWI76_RS04635 at 966.152 kb on + strand, within BWI76_RS04635 at 966.152 kb on + strand, within BWI76_RS04635 at 966.152 kb on + strand, within BWI76_RS04635 at 966.152 kb on + strand, within BWI76_RS04635 at 966.160 kb on - strand, within BWI76_RS04635 at 966.160 kb on - strand, within BWI76_RS04635 at 966.160 kb on - strand, within BWI76_RS04635 at 966.160 kb on - strand, within BWI76_RS04635 at 966.160 kb on - strand, within BWI76_RS04635 at 966.164 kb on - strand, within BWI76_RS04635 at 966.187 kb on + strand, within BWI76_RS04635 at 966.195 kb on - strand, within BWI76_RS04635 at 966.200 kb on + strand, within BWI76_RS04635 at 966.202 kb on + strand, within BWI76_RS04635 at 966.207 kb on + strand, within BWI76_RS04635 at 966.214 kb on + strand, within BWI76_RS04635 at 966.217 kb on - strand, within BWI76_RS04635 at 966.219 kb on + strand, within BWI76_RS04635 at 966.220 kb on + strand, within BWI76_RS04635 at 966.220 kb on + strand, within BWI76_RS04635 at 966.220 kb on + strand, within BWI76_RS04635 at 966.222 kb on - strand, within BWI76_RS04635 at 966.222 kb on - strand, within BWI76_RS04635 at 966.225 kb on + strand, within BWI76_RS04635 at 966.226 kb on + strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.228 kb on - strand, within BWI76_RS04635 at 966.229 kb on - strand, within BWI76_RS04635 at 966.229 kb on - strand at 966.232 kb on + strand, within BWI76_RS04635 at 966.233 kb on - strand, within BWI76_RS04635 at 966.233 kb on - strand, within BWI76_RS04635
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 963,194 + BWI76_RS04620 0.77 -0.2 963,230 + BWI76_RS04620 0.79 +1.1 963,364 + BWI76_RS04620 0.87 -0.2 963,364 + BWI76_RS04620 0.87 -0.4 963,397 + BWI76_RS04620 0.89 +0.1 963,774 + BWI76_RS04625 0.71 -0.8 963,785 + BWI76_RS04625 0.78 +0.5 963,805 + -1.2 963,805 + +0.7 963,813 - +0.2 963,827 + -0.3 963,827 + +1.2 963,827 + +0.6 963,975 + +0.4 963,975 + +0.6 963,975 + +0.4 964,159 + +0.3 964,203 + BWI76_RS04630 0.13 +0.8 964,208 + BWI76_RS04630 0.13 +1.1 964,246 + BWI76_RS04630 0.17 -0.4 964,263 + BWI76_RS04630 0.18 +0.2 964,365 - BWI76_RS04630 0.27 -1.9 964,365 - BWI76_RS04630 0.27 +0.8 964,386 - BWI76_RS04630 0.28 +0.2 964,386 - BWI76_RS04630 0.28 +1.0 964,464 + BWI76_RS04630 0.35 +0.9 964,496 + BWI76_RS04630 0.38 -0.4 964,524 + BWI76_RS04630 0.40 +0.5 964,593 + BWI76_RS04630 0.46 -1.4 964,595 + BWI76_RS04630 0.46 +0.3 964,600 + BWI76_RS04630 0.46 -0.3 964,658 - BWI76_RS04630 0.51 +0.1 964,807 + BWI76_RS04630 0.64 +1.9 964,900 + BWI76_RS04630 0.72 +0.1 964,942 - BWI76_RS04630 0.75 +0.7 964,953 - BWI76_RS04630 0.76 -0.6 964,966 + BWI76_RS04630 0.77 +1.2 964,966 + BWI76_RS04630 0.77 -1.0 964,991 + BWI76_RS04630 0.79 +0.7 964,999 - BWI76_RS04630 0.80 -1.2 964,999 - BWI76_RS04630 0.80 +0.8 965,063 + BWI76_RS04630 0.85 -2.6 965,071 - BWI76_RS04630 0.86 -0.4 965,122 + +1.3 965,125 - +0.1 965,125 - -0.4 965,126 + +0.9 965,141 + +0.8 965,141 + -0.4 965,149 + +0.3 965,149 + +0.6 965,149 - +1.7 965,150 - -0.4 965,157 - +0.2 965,159 + +0.5 965,159 + -1.2 965,159 + +0.4 965,167 - -0.7 965,204 + +0.3 965,204 + +1.1 965,204 + -0.0 965,204 + +0.8 965,204 + +0.4 965,204 + -1.7 965,204 + +0.6 965,204 + -0.5 965,204 + -0.1 965,204 + +1.0 965,204 + -0.3 965,204 + +0.2 965,212 - -0.9 965,212 - -0.9 965,212 - +0.4 965,212 - -1.4 965,229 + -0.8 965,251 + +0.3 965,260 + +1.1 965,266 - +0.4 965,267 - +0.7 965,269 + -1.7 965,272 - -0.9 965,277 - +0.3 965,277 - +0.4 965,277 - +0.2 965,277 - +0.0 965,277 - +1.2 965,277 - +2.2 965,277 - +1.0 965,277 - -1.9 965,287 - +0.1 965,449 + BWI76_RS04635 0.13 -1.2 965,522 + BWI76_RS04635 0.18 -0.7 965,534 - BWI76_RS04635 0.19 -1.2 965,570 + BWI76_RS04635 0.22 +1.1 965,570 + BWI76_RS04635 0.22 +1.1 965,570 + +1.1 965,578 - BWI76_RS04635 0.23 +3.9 965,580 + -0.9 965,595 + BWI76_RS04635 0.24 +1.1 965,595 + BWI76_RS04635 0.24 +1.1 965,595 + BWI76_RS04635 0.24 +0.7 965,595 + BWI76_RS04635 0.24 -1.7 965,595 + -1.2 965,595 + BWI76_RS04635 0.24 -2.0 965,595 + BWI76_RS04635 0.24 -0.5 965,603 - BWI76_RS04635 0.25 -2.5 965,603 - +1.1 965,603 - BWI76_RS04635 0.25 -1.7 965,603 - BWI76_RS04635 0.25 +2.1 965,603 - BWI76_RS04635 0.25 +0.1 965,603 - BWI76_RS04635 0.25 -1.7 965,603 - BWI76_RS04635 0.25 -0.2 965,613 - BWI76_RS04635 0.25 -2.2 965,728 - BWI76_RS04635 0.34 -2.0 965,728 - BWI76_RS04635 0.34 -1.2 965,730 + BWI76_RS04635 0.34 +1.7 965,736 - +0.1 965,738 - BWI76_RS04635 0.35 +0.5 965,738 - BWI76_RS04635 0.35 -1.2 965,751 + BWI76_RS04635 0.36 +1.1 965,755 - BWI76_RS04635 0.36 +0.2 965,789 + BWI76_RS04635 0.39 -0.5 965,797 - BWI76_RS04635 0.39 -1.1 965,797 - BWI76_RS04635 0.39 -0.5 965,797 - BWI76_RS04635 0.39 -2.1 965,804 + BWI76_RS04635 0.40 +0.5 965,807 + BWI76_RS04635 0.40 -3.0 965,815 - BWI76_RS04635 0.41 -1.7 965,816 + BWI76_RS04635 0.41 -2.3 965,817 - BWI76_RS04635 0.41 -0.8 965,846 - BWI76_RS04635 0.43 -0.9 965,846 - BWI76_RS04635 0.43 +0.2 965,862 + BWI76_RS04635 0.44 -2.9 965,869 + BWI76_RS04635 0.45 +0.3 965,872 + BWI76_RS04635 0.45 -0.7 965,872 + +1.1 965,872 + BWI76_RS04635 0.45 +1.1 965,873 + BWI76_RS04635 0.45 -0.5 965,877 - BWI76_RS04635 0.45 -2.6 965,881 + BWI76_RS04635 0.46 -0.4 965,881 + BWI76_RS04635 0.46 -0.1 965,881 + BWI76_RS04635 0.46 +1.2 965,889 - BWI76_RS04635 0.46 +0.1 965,889 - BWI76_RS04635 0.46 +1.1 965,896 + BWI76_RS04635 0.47 -0.5 965,904 - BWI76_RS04635 0.48 -0.5 965,904 - BWI76_RS04635 0.48 -1.2 965,904 - BWI76_RS04635 0.48 +0.1 965,904 - BWI76_RS04635 0.48 +0.4 965,947 - BWI76_RS04635 0.51 -0.7 965,955 - BWI76_RS04635 0.51 -2.0 965,955 - BWI76_RS04635 0.51 -0.9 965,990 + BWI76_RS04635 0.54 -0.9 966,069 - BWI76_RS04635 0.60 -0.4 966,108 + BWI76_RS04635 0.63 -1.7 966,108 + BWI76_RS04635 0.63 -0.7 966,108 + BWI76_RS04635 0.63 +2.3 966,108 + BWI76_RS04635 0.63 +1.9 966,116 - BWI76_RS04635 0.64 +1.1 966,126 + BWI76_RS04635 0.64 -0.9 966,136 - BWI76_RS04635 0.65 +2.4 966,152 + BWI76_RS04635 0.66 -0.4 966,152 + BWI76_RS04635 0.66 -0.2 966,152 + BWI76_RS04635 0.66 +1.1 966,152 + BWI76_RS04635 0.66 -3.2 966,160 - BWI76_RS04635 0.67 -1.5 966,160 - BWI76_RS04635 0.67 +0.9 966,160 - BWI76_RS04635 0.67 -1.9 966,160 - BWI76_RS04635 0.67 -2.0 966,160 - BWI76_RS04635 0.67 +0.7 966,164 - BWI76_RS04635 0.67 +0.1 966,187 + BWI76_RS04635 0.69 -0.5 966,195 - BWI76_RS04635 0.70 -0.9 966,200 + BWI76_RS04635 0.70 -1.2 966,202 + BWI76_RS04635 0.70 -1.5 966,207 + BWI76_RS04635 0.71 +1.4 966,214 + BWI76_RS04635 0.71 -2.9 966,217 - BWI76_RS04635 0.71 -3.5 966,219 + BWI76_RS04635 0.72 -2.2 966,220 + BWI76_RS04635 0.72 -1.5 966,220 + BWI76_RS04635 0.72 +1.5 966,220 + BWI76_RS04635 0.72 -3.3 966,222 - BWI76_RS04635 0.72 -0.5 966,222 - BWI76_RS04635 0.72 +2.9 966,225 + BWI76_RS04635 0.72 -1.5 966,226 + BWI76_RS04635 0.72 -3.0 966,228 - BWI76_RS04635 0.72 +2.7 966,228 - -0.5 966,228 - BWI76_RS04635 0.72 +0.1 966,228 - BWI76_RS04635 0.72 -0.4 966,228 - BWI76_RS04635 0.72 +0.5 966,228 - BWI76_RS04635 0.72 -0.3 966,228 - BWI76_RS04635 0.72 +1.0 966,228 - BWI76_RS04635 0.72 +2.5 966,228 - BWI76_RS04635 0.72 -0.0 966,228 - BWI76_RS04635 0.72 -0.2 966,229 - BWI76_RS04635 0.72 -2.6 966,229 - -1.2 966,232 + BWI76_RS04635 0.73 +1.1 966,233 - BWI76_RS04635 0.73 -1.2 966,233 - BWI76_RS04635 0.73 +0.7
Or see this region's nucleotide sequence