Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04140 and BWI76_RS04145 are separated by 94 nucleotides BWI76_RS04145 and BWI76_RS04150 are separated by 58 nucleotides BWI76_RS04150 and BWI76_RS04155 are separated by 10 nucleotides BWI76_RS04155 and BWI76_RS04160 are separated by 104 nucleotides
BWI76_RS04140: BWI76_RS04140 - phosphopentomutase, at 851,800 to 853,023
_RS04140
BWI76_RS04145: BWI76_RS04145 - purine-nucleoside phosphorylase, at 853,118 to 853,840
_RS04145
BWI76_RS04150: BWI76_RS04150 - lipoate--protein ligase A, at 853,899 to 854,915
_RS04150
BWI76_RS04155: BWI76_RS04155 - membrane protein, at 854,926 to 855,567
_RS04155
BWI76_RS04160: BWI76_RS04160 - phosphoserine phosphatase, at 855,672 to 856,643
_RS04160
Position (kb) 853
854
855 Strain fitness (log2 ratio) -2
-1
0
1 at 852.948 kb on - strand at 853.171 kb on + strand at 853.670 kb on + strand, within BWI76_RS04145 at 853.696 kb on - strand, within BWI76_RS04145 at 853.751 kb on + strand, within BWI76_RS04145 at 853.789 kb on - strand at 853.815 kb on + strand at 854.125 kb on - strand, within BWI76_RS04150 at 854.125 kb on - strand, within BWI76_RS04150 at 854.264 kb on - strand, within BWI76_RS04150 at 854.274 kb on - strand, within BWI76_RS04150 at 854.379 kb on + strand, within BWI76_RS04150 at 854.386 kb on + strand, within BWI76_RS04150 at 854.387 kb on - strand, within BWI76_RS04150 at 854.505 kb on + strand, within BWI76_RS04150 at 854.513 kb on - strand, within BWI76_RS04150 at 854.670 kb on - strand, within BWI76_RS04150 at 854.972 kb on - strand at 855.070 kb on + strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.251 kb on - strand, within BWI76_RS04155 at 855.486 kb on + strand, within BWI76_RS04155 at 855.504 kb on - strand at 855.512 kb on - strand at 855.522 kb on - strand at 855.691 kb on + strand at 855.726 kb on + strand at 855.797 kb on + strand, within BWI76_RS04160 at 855.866 kb on - strand, within BWI76_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 852,948 - -1.7 853,171 + +0.2 853,670 + BWI76_RS04145 0.76 -0.1 853,696 - BWI76_RS04145 0.80 +0.0 853,751 + BWI76_RS04145 0.88 -0.9 853,789 - -0.5 853,815 + +0.6 854,125 - BWI76_RS04150 0.22 +0.1 854,125 - BWI76_RS04150 0.22 +0.6 854,264 - BWI76_RS04150 0.36 -0.9 854,274 - BWI76_RS04150 0.37 -0.1 854,379 + BWI76_RS04150 0.47 -1.8 854,386 + BWI76_RS04150 0.48 +1.6 854,387 - BWI76_RS04150 0.48 -0.8 854,505 + BWI76_RS04150 0.60 -0.2 854,513 - BWI76_RS04150 0.60 -2.3 854,670 - BWI76_RS04150 0.76 -0.3 854,972 - +0.3 855,070 + BWI76_RS04155 0.22 +0.5 855,251 - BWI76_RS04155 0.51 -0.0 855,251 - BWI76_RS04155 0.51 +0.5 855,486 + BWI76_RS04155 0.87 +1.2 855,504 - -2.0 855,512 - -0.9 855,522 - +0.3 855,691 + -0.4 855,726 + -2.2 855,797 + BWI76_RS04160 0.13 -0.4 855,866 - BWI76_RS04160 0.20 -1.4
Or see this region's nucleotide sequence