Experiment: RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00500 and BWI76_RS00505 are separated by 11 nucleotides BWI76_RS00505 and BWI76_RS00510 are separated by 13 nucleotides BWI76_RS00510 and BWI76_RS00515 overlap by 4 nucleotides BWI76_RS00515 and BWI76_RS00520 are separated by 175 nucleotides
BWI76_RS00500: BWI76_RS00500 - PTS sorbose IIC component, at 102,606 to 103,409
_RS00500
BWI76_RS00505: BWI76_RS00505 - PTS system, sorbose-specific IIB component, at 103,421 to 103,918
_RS00505
BWI76_RS00510: BWI76_RS00510 - glucuronyl hydrolase, at 103,932 to 105,110
_RS00510
BWI76_RS00515: BWI76_RS00515 - PTS fructose transporter subunit IIA, at 105,107 to 105,589
_RS00515
BWI76_RS00520: BWI76_RS00520 - transcription regulator of PTS system, at 105,765 to 108,368
_RS00520
Position (kb) 103
104
105
106 Strain fitness (log2 ratio) -2
-1
0
1
2 at 102.936 kb on - strand, within BWI76_RS00500 at 102.985 kb on + strand, within BWI76_RS00500 at 103.017 kb on + strand, within BWI76_RS00500 at 103.025 kb on - strand, within BWI76_RS00500 at 103.058 kb on + strand, within BWI76_RS00500 at 103.063 kb on + strand, within BWI76_RS00500 at 103.063 kb on + strand, within BWI76_RS00500 at 103.066 kb on - strand, within BWI76_RS00500 at 103.071 kb on - strand, within BWI76_RS00500 at 103.302 kb on - strand, within BWI76_RS00500 at 103.309 kb on + strand, within BWI76_RS00500 at 103.346 kb on + strand at 103.346 kb on + strand at 103.360 kb on + strand at 103.505 kb on + strand, within BWI76_RS00505 at 103.720 kb on - strand, within BWI76_RS00505 at 103.824 kb on - strand, within BWI76_RS00505 at 103.845 kb on + strand, within BWI76_RS00505 at 103.865 kb on - strand, within BWI76_RS00505 at 103.933 kb on - strand at 103.944 kb on + strand at 103.963 kb on - strand at 103.968 kb on + strand at 104.111 kb on + strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.119 kb on - strand, within BWI76_RS00510 at 104.223 kb on - strand, within BWI76_RS00510 at 104.265 kb on + strand, within BWI76_RS00510 at 104.341 kb on + strand, within BWI76_RS00510 at 104.347 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.349 kb on - strand, within BWI76_RS00510 at 104.460 kb on - strand, within BWI76_RS00510 at 104.654 kb on - strand, within BWI76_RS00510 at 104.727 kb on - strand, within BWI76_RS00510 at 104.765 kb on - strand, within BWI76_RS00510 at 104.772 kb on - strand, within BWI76_RS00510 at 104.796 kb on - strand, within BWI76_RS00510 at 104.829 kb on + strand at 105.106 kb on + strand at 105.113 kb on - strand at 105.124 kb on - strand at 105.161 kb on - strand, within BWI76_RS00515 at 105.201 kb on + strand, within BWI76_RS00515 at 105.312 kb on + strand, within BWI76_RS00515 at 105.444 kb on - strand, within BWI76_RS00515 at 105.457 kb on - strand, within BWI76_RS00515 at 105.512 kb on - strand, within BWI76_RS00515 at 105.526 kb on + strand, within BWI76_RS00515 at 105.614 kb on - strand at 105.726 kb on - strand at 105.734 kb on - strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.762 kb on + strand at 105.763 kb on + strand at 105.770 kb on - strand at 105.770 kb on - strand at 105.771 kb on - strand at 105.771 kb on - strand at 105.784 kb on - strand at 105.855 kb on + strand at 105.940 kb on - strand at 106.051 kb on + strand, within BWI76_RS00520
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Pseudomonas simiae WCS417 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 102,936 - BWI76_RS00500 0.41 -1.4 102,985 + BWI76_RS00500 0.47 +0.7 103,017 + BWI76_RS00500 0.51 -1.5 103,025 - BWI76_RS00500 0.52 -0.4 103,058 + BWI76_RS00500 0.56 +0.5 103,063 + BWI76_RS00500 0.57 -2.6 103,063 + BWI76_RS00500 0.57 -0.8 103,066 - BWI76_RS00500 0.57 +0.2 103,071 - BWI76_RS00500 0.58 -0.0 103,302 - BWI76_RS00500 0.87 +0.5 103,309 + BWI76_RS00500 0.87 +1.3 103,346 + +0.1 103,346 + +0.3 103,360 + -0.1 103,505 + BWI76_RS00505 0.17 -0.3 103,720 - BWI76_RS00505 0.60 +1.3 103,824 - BWI76_RS00505 0.81 +0.7 103,845 + BWI76_RS00505 0.85 +0.4 103,865 - BWI76_RS00505 0.89 +2.4 103,933 - -0.3 103,944 + +0.5 103,963 - +0.9 103,968 + +0.6 104,111 + BWI76_RS00510 0.15 -2.5 104,119 - BWI76_RS00510 0.16 -1.7 104,119 - BWI76_RS00510 0.16 -0.1 104,119 - BWI76_RS00510 0.16 +1.3 104,223 - BWI76_RS00510 0.25 -0.5 104,265 + BWI76_RS00510 0.28 +0.5 104,341 + BWI76_RS00510 0.35 +1.3 104,347 - BWI76_RS00510 0.35 +0.2 104,349 - BWI76_RS00510 0.35 +0.5 104,349 - BWI76_RS00510 0.35 -0.3 104,460 - BWI76_RS00510 0.45 -0.8 104,654 - BWI76_RS00510 0.61 +2.4 104,727 - BWI76_RS00510 0.67 -1.9 104,765 - BWI76_RS00510 0.71 +0.0 104,772 - BWI76_RS00510 0.71 -1.5 104,796 - BWI76_RS00510 0.73 -1.9 104,829 + +1.3 105,106 + -0.9 105,113 - -1.0 105,124 - -0.7 105,161 - BWI76_RS00515 0.11 +0.3 105,201 + BWI76_RS00515 0.19 -2.3 105,312 + BWI76_RS00515 0.42 +0.0 105,444 - BWI76_RS00515 0.70 -0.4 105,457 - BWI76_RS00515 0.72 +1.3 105,512 - BWI76_RS00515 0.84 -0.3 105,526 + BWI76_RS00515 0.87 -1.7 105,614 - +0.9 105,726 - -0.8 105,734 - +0.6 105,762 + -2.0 105,762 + +1.3 105,762 + -0.9 105,763 + +1.0 105,770 - +0.6 105,770 - +0.7 105,771 - -0.7 105,771 - +0.5 105,784 - -0.3 105,855 + -0.1 105,940 - +1.3 106,051 + BWI76_RS00520 0.11 +0.3
Or see this region's nucleotide sequence