Experiment: 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5478 and Pf6N2E2_5479 overlap by 4 nucleotides Pf6N2E2_5479 and Pf6N2E2_5480 are separated by 15 nucleotides Pf6N2E2_5480 and Pf6N2E2_5481 are separated by 25 nucleotides
Pf6N2E2_5478 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,296,331 to 6,297,197
_5478
Pf6N2E2_5479 - ABC spermidine/putrescine transporter, inner membrane subunit, at 6,297,194 to 6,298,042
_5479
Pf6N2E2_5480 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 6,298,058 to 6,299,113
_5480
Pf6N2E2_5481 - Transcriptional regulator, AraC family, at 6,299,139 to 6,300,068
_5481
Position (kb)
6298
6299
6300 Strain fitness (log2 ratio)
-1
0
1
2 at 6297.153 kb on + strand at 6297.360 kb on - strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.872 kb on + strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.873 kb on - strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.881 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.917 kb on + strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6297.918 kb on - strand, within Pf6N2E2_5479 at 6298.071 kb on + strand at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.648 kb on + strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.649 kb on - strand, within Pf6N2E2_5480 at 6298.722 kb on - strand, within Pf6N2E2_5480 at 6299.026 kb on + strand at 6299.026 kb on + strand at 6299.027 kb on - strand at 6299.027 kb on - strand at 6299.080 kb on - strand at 6299.200 kb on + strand at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.317 kb on + strand, within Pf6N2E2_5481 at 6299.528 kb on - strand, within Pf6N2E2_5481 at 6299.580 kb on - strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.641 kb on + strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.642 kb on - strand, within Pf6N2E2_5481 at 6299.671 kb on - strand, within Pf6N2E2_5481 at 6299.809 kb on + strand, within Pf6N2E2_5481 at 6299.824 kb on + strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.825 kb on - strand, within Pf6N2E2_5481 at 6299.912 kb on + strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.913 kb on - strand, within Pf6N2E2_5481 at 6299.962 kb on + strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6299.963 kb on - strand, within Pf6N2E2_5481 at 6300.028 kb on + strand at 6300.029 kb on - strand at 6300.029 kb on - strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.109 kb on + strand at 6300.110 kb on - strand at 6300.110 kb on - strand at 6300.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine remove 6,297,153 + -0.2 6,297,360 - Pf6N2E2_5479 0.20 +0.1 6,297,872 + Pf6N2E2_5479 0.80 -0.4 6,297,872 + Pf6N2E2_5479 0.80 +1.2 6,297,873 - Pf6N2E2_5479 0.80 +2.1 6,297,873 - Pf6N2E2_5479 0.80 -0.9 6,297,873 - Pf6N2E2_5479 0.80 +0.1 6,297,873 - Pf6N2E2_5479 0.80 -0.1 6,297,881 + Pf6N2E2_5479 0.81 -0.4 6,297,881 + Pf6N2E2_5479 0.81 +0.7 6,297,917 + Pf6N2E2_5479 0.85 +0.5 6,297,917 + Pf6N2E2_5479 0.85 +1.1 6,297,917 + Pf6N2E2_5479 0.85 +0.3 6,297,917 + Pf6N2E2_5479 0.85 +0.0 6,297,918 - Pf6N2E2_5479 0.85 +1.7 6,297,918 - Pf6N2E2_5479 0.85 +0.6 6,298,071 + +1.2 6,298,648 + Pf6N2E2_5480 0.56 +1.1 6,298,648 + Pf6N2E2_5480 0.56 -0.1 6,298,648 + Pf6N2E2_5480 0.56 +0.9 6,298,648 + Pf6N2E2_5480 0.56 -0.7 6,298,648 + Pf6N2E2_5480 0.56 -0.1 6,298,649 - Pf6N2E2_5480 0.56 +0.9 6,298,649 - Pf6N2E2_5480 0.56 +0.1 6,298,649 - Pf6N2E2_5480 0.56 +0.6 6,298,649 - Pf6N2E2_5480 0.56 +0.1 6,298,649 - Pf6N2E2_5480 0.56 +0.9 6,298,722 - Pf6N2E2_5480 0.63 +0.4 6,299,026 + +0.0 6,299,026 + +0.5 6,299,027 - +0.9 6,299,027 - -0.4 6,299,080 - +0.9 6,299,200 + +1.7 6,299,317 + Pf6N2E2_5481 0.19 +0.4 6,299,317 + Pf6N2E2_5481 0.19 +0.9 6,299,317 + Pf6N2E2_5481 0.19 +0.1 6,299,528 - Pf6N2E2_5481 0.42 +0.7 6,299,580 - Pf6N2E2_5481 0.47 +0.6 6,299,641 + Pf6N2E2_5481 0.54 -0.1 6,299,641 + Pf6N2E2_5481 0.54 +0.2 6,299,642 - Pf6N2E2_5481 0.54 +0.9 6,299,642 - Pf6N2E2_5481 0.54 +0.6 6,299,642 - Pf6N2E2_5481 0.54 -0.3 6,299,642 - Pf6N2E2_5481 0.54 +0.6 6,299,671 - Pf6N2E2_5481 0.57 -1.0 6,299,809 + Pf6N2E2_5481 0.72 +0.1 6,299,824 + Pf6N2E2_5481 0.74 -0.4 6,299,825 - Pf6N2E2_5481 0.74 +0.7 6,299,825 - Pf6N2E2_5481 0.74 +0.4 6,299,825 - Pf6N2E2_5481 0.74 +1.5 6,299,825 - Pf6N2E2_5481 0.74 -0.1 6,299,912 + Pf6N2E2_5481 0.83 -0.9 6,299,913 - Pf6N2E2_5481 0.83 +0.1 6,299,913 - Pf6N2E2_5481 0.83 +1.0 6,299,962 + Pf6N2E2_5481 0.88 -0.3 6,299,963 - Pf6N2E2_5481 0.89 -0.9 6,299,963 - Pf6N2E2_5481 0.89 -0.2 6,299,963 - Pf6N2E2_5481 0.89 +0.7 6,300,028 + +0.1 6,300,029 - +0.3 6,300,029 - +0.8 6,300,109 + +0.1 6,300,109 + +0.1 6,300,109 + +0.9 6,300,110 - +1.4 6,300,110 - +0.6 6,300,110 - +2.3
Or see this region's nucleotide sequence