Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25040

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25030 and BWI76_RS25035 overlap by 1 nucleotidesBWI76_RS25035 and BWI76_RS25040 are separated by 43 nucleotidesBWI76_RS25040 and BWI76_RS25045 overlap by 38 nucleotides BWI76_RS25030: BWI76_RS25030 - malonate transporter subunit MadL, at 5,211,287 to 5,211,682 _RS25030 BWI76_RS25035: BWI76_RS25035 - malonate transporter subunit MadM, at 5,211,682 to 5,212,452 _RS25035 BWI76_RS25040: BWI76_RS25040 - hypothetical protein, at 5,212,496 to 5,212,927 _RS25040 BWI76_RS25045: BWI76_RS25045 - Malonate utilization transacting regulator MdcR, at 5,212,890 to 5,213,831 _RS25045 Position (kb) 5212 5213Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 5211.536 kb on - strand, within BWI76_RS25030at 5211.537 kb on - strandat 5211.547 kb on + strand, within BWI76_RS25030at 5211.547 kb on + strand, within BWI76_RS25030at 5211.610 kb on + strand, within BWI76_RS25030at 5211.610 kb on + strand, within BWI76_RS25030at 5211.793 kb on - strand, within BWI76_RS25035at 5211.849 kb on + strand, within BWI76_RS25035at 5211.900 kb on - strand, within BWI76_RS25035at 5211.922 kb on - strand, within BWI76_RS25035at 5212.129 kb on + strand, within BWI76_RS25035at 5212.225 kb on + strand, within BWI76_RS25035at 5212.391 kb on - strandat 5212.433 kb on + strandat 5212.487 kb on - strandat 5212.495 kb on + strandat 5212.503 kb on + strandat 5212.543 kb on - strand, within BWI76_RS25040at 5212.607 kb on - strand, within BWI76_RS25040at 5212.613 kb on - strand, within BWI76_RS25040at 5212.669 kb on - strand, within BWI76_RS25040at 5212.673 kb on + strand, within BWI76_RS25040at 5212.681 kb on - strand, within BWI76_RS25040at 5212.681 kb on - strand, within BWI76_RS25040at 5212.681 kb on - strand, within BWI76_RS25040at 5212.741 kb on + strand, within BWI76_RS25040at 5212.746 kb on + strand, within BWI76_RS25040at 5212.762 kb on - strand, within BWI76_RS25040at 5212.767 kb on - strandat 5212.852 kb on + strandat 5212.881 kb on + strand, within BWI76_RS25040at 5212.896 kb on - strandat 5212.921 kb on + strandat 5212.921 kb on + strandat 5212.929 kb on - strandat 5212.929 kb on - strandat 5212.943 kb on - strandat 5212.965 kb on + strandat 5212.970 kb on - strandat 5212.970 kb on - strandat 5213.026 kb on - strand, within BWI76_RS25045at 5213.040 kb on - strand, within BWI76_RS25045at 5213.051 kb on + strand, within BWI76_RS25045at 5213.059 kb on - strandat 5213.059 kb on - strand, within BWI76_RS25045at 5213.059 kb on - strand, within BWI76_RS25045at 5213.067 kb on - strand, within BWI76_RS25045at 5213.070 kb on - strand, within BWI76_RS25045at 5213.070 kb on - strand, within BWI76_RS25045at 5213.072 kb on - strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.097 kb on + strand, within BWI76_RS25045at 5213.098 kb on + strand, within BWI76_RS25045at 5213.098 kb on + strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.105 kb on - strand, within BWI76_RS25045at 5213.120 kb on - strand, within BWI76_RS25045at 5213.122 kb on + strand, within BWI76_RS25045at 5213.125 kb on - strand, within BWI76_RS25045at 5213.130 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.164 kb on - strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.195 kb on + strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.203 kb on - strand, within BWI76_RS25045at 5213.209 kb on - strand, within BWI76_RS25045at 5213.209 kb on - strand, within BWI76_RS25045at 5213.220 kb on + strand, within BWI76_RS25045at 5213.220 kb on + strand, within BWI76_RS25045at 5213.220 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.228 kb on - strand, within BWI76_RS25045at 5213.229 kb on - strand, within BWI76_RS25045at 5213.233 kb on + strand, within BWI76_RS25045at 5213.267 kb on - strand, within BWI76_RS25045at 5213.272 kb on - strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on + strand, within BWI76_RS25045at 5213.275 kb on - strand, within BWI76_RS25045at 5213.283 kb on + strand, within BWI76_RS25045at 5213.284 kb on - strand, within BWI76_RS25045at 5213.288 kb on + strand, within BWI76_RS25045at 5213.291 kb on - strand, within BWI76_RS25045at 5213.298 kb on + strand, within BWI76_RS25045at 5213.306 kb on + strandat 5213.306 kb on - strand, within BWI76_RS25045at 5213.306 kb on - strand, within BWI76_RS25045at 5213.314 kb on - strand, within BWI76_RS25045at 5213.314 kb on - strand, within BWI76_RS25045at 5213.372 kb on - strand, within BWI76_RS25045at 5213.374 kb on + strand, within BWI76_RS25045at 5213.459 kb on + strand, within BWI76_RS25045at 5213.459 kb on + strand, within BWI76_RS25045at 5213.467 kb on - strandat 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.467 kb on - strand, within BWI76_RS25045at 5213.503 kb on + strand, within BWI76_RS25045at 5213.523 kb on + strand, within BWI76_RS25045at 5213.536 kb on + strand, within BWI76_RS25045at 5213.548 kb on + strand, within BWI76_RS25045at 5213.604 kb on - strand, within BWI76_RS25045at 5213.630 kb on + strand, within BWI76_RS25045at 5213.638 kb on - strandat 5213.675 kb on + strand, within BWI76_RS25045at 5213.675 kb on + strand, within BWI76_RS25045at 5213.717 kb on + strand, within BWI76_RS25045at 5213.774 kb on + strandat 5213.777 kb on + strandat 5213.777 kb on + strandat 5213.777 kb on + strandat 5213.782 kb on - strandat 5213.785 kb on - strandat 5213.810 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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5,211,536 - BWI76_RS25030 0.63 -0.5
5,211,537 - +1.1
5,211,547 + BWI76_RS25030 0.66 -2.6
5,211,547 + BWI76_RS25030 0.66 +0.9
5,211,610 + BWI76_RS25030 0.82 -0.7
5,211,610 + BWI76_RS25030 0.82 -1.6
5,211,793 - BWI76_RS25035 0.14 +0.1
5,211,849 + BWI76_RS25035 0.22 +0.3
5,211,900 - BWI76_RS25035 0.28 +0.1
5,211,922 - BWI76_RS25035 0.31 +0.5
5,212,129 + BWI76_RS25035 0.58 +0.1
5,212,225 + BWI76_RS25035 0.70 +0.5
5,212,391 - -3.8
5,212,433 + +0.2
5,212,487 - +0.4
5,212,495 + -0.3
5,212,503 + +1.1
5,212,543 - BWI76_RS25040 0.11 -1.1
5,212,607 - BWI76_RS25040 0.26 -2.7
5,212,613 - BWI76_RS25040 0.27 -0.1
5,212,669 - BWI76_RS25040 0.40 +0.4
5,212,673 + BWI76_RS25040 0.41 -2.6
5,212,681 - BWI76_RS25040 0.43 -1.5
5,212,681 - BWI76_RS25040 0.43 -0.5
5,212,681 - BWI76_RS25040 0.43 +0.1
5,212,741 + BWI76_RS25040 0.57 +0.1
5,212,746 + BWI76_RS25040 0.58 -3.2
5,212,762 - BWI76_RS25040 0.62 +0.6
5,212,767 - +2.3
5,212,852 + +0.1
5,212,881 + BWI76_RS25040 0.89 -0.3
5,212,896 - +1.1
5,212,921 + -1.2
5,212,921 + -0.9
5,212,929 - +0.6
5,212,929 - -2.8
5,212,943 - -0.8
5,212,965 + -0.4
5,212,970 - -0.5
5,212,970 - +0.4
5,213,026 - BWI76_RS25045 0.14 -0.4
5,213,040 - BWI76_RS25045 0.16 -1.7
5,213,051 + BWI76_RS25045 0.17 -0.5
5,213,059 - -0.9
5,213,059 - BWI76_RS25045 0.18 +1.1
5,213,059 - BWI76_RS25045 0.18 -0.1
5,213,067 - BWI76_RS25045 0.19 -0.0
5,213,070 - BWI76_RS25045 0.19 -1.9
5,213,070 - BWI76_RS25045 0.19 -2.6
5,213,072 - BWI76_RS25045 0.19 -0.6
5,213,097 + BWI76_RS25045 0.22 +0.1
5,213,097 + BWI76_RS25045 0.22 -0.7
5,213,097 + BWI76_RS25045 0.22 -0.5
5,213,097 + BWI76_RS25045 0.22 +1.5
5,213,097 + BWI76_RS25045 0.22 +2.3
5,213,098 + BWI76_RS25045 0.22 -1.7
5,213,098 + BWI76_RS25045 0.22 +1.1
5,213,105 - BWI76_RS25045 0.23 +1.0
5,213,105 - BWI76_RS25045 0.23 +0.1
5,213,105 - BWI76_RS25045 0.23 -1.2
5,213,105 - BWI76_RS25045 0.23 -1.5
5,213,105 - BWI76_RS25045 0.23 -0.4
5,213,105 - BWI76_RS25045 0.23 +2.9
5,213,105 - BWI76_RS25045 0.23 +0.4
5,213,105 - BWI76_RS25045 0.23 +0.2
5,213,105 - BWI76_RS25045 0.23 -2.2
5,213,105 - BWI76_RS25045 0.23 +0.0
5,213,105 - BWI76_RS25045 0.23 -2.3
5,213,120 - BWI76_RS25045 0.24 +2.2
5,213,122 + BWI76_RS25045 0.25 -0.3
5,213,125 - BWI76_RS25045 0.25 +0.9
5,213,130 - BWI76_RS25045 0.25 +0.3
5,213,164 - BWI76_RS25045 0.29 +0.3
5,213,164 - BWI76_RS25045 0.29 -1.3
5,213,164 - BWI76_RS25045 0.29 -3.5
5,213,164 - BWI76_RS25045 0.29 +0.3
5,213,164 - BWI76_RS25045 0.29 -0.0
5,213,195 + BWI76_RS25045 0.32 -0.8
5,213,195 + BWI76_RS25045 0.32 +0.1
5,213,195 + BWI76_RS25045 0.32 -3.3
5,213,195 + BWI76_RS25045 0.32 -3.0
5,213,203 - BWI76_RS25045 0.33 -1.2
5,213,203 - BWI76_RS25045 0.33 +0.1
5,213,203 - BWI76_RS25045 0.33 -2.1
5,213,203 - BWI76_RS25045 0.33 -0.9
5,213,209 - BWI76_RS25045 0.34 +0.3
5,213,209 - BWI76_RS25045 0.34 -0.6
5,213,220 + BWI76_RS25045 0.35 -0.9
5,213,220 + BWI76_RS25045 0.35 -2.4
5,213,220 - BWI76_RS25045 0.35 +1.4
5,213,228 - BWI76_RS25045 0.36 -1.7
5,213,228 - BWI76_RS25045 0.36 +0.0
5,213,228 - BWI76_RS25045 0.36 -0.9
5,213,229 - BWI76_RS25045 0.36 +0.1
5,213,233 + BWI76_RS25045 0.36 +0.3
5,213,267 - BWI76_RS25045 0.40 -0.2
5,213,272 - BWI76_RS25045 0.41 -3.3
5,213,275 + BWI76_RS25045 0.41 +1.3
5,213,275 + BWI76_RS25045 0.41 +0.7
5,213,275 + BWI76_RS25045 0.41 -0.9
5,213,275 - BWI76_RS25045 0.41 +1.1
5,213,283 + BWI76_RS25045 0.42 -0.5
5,213,284 - BWI76_RS25045 0.42 +0.1
5,213,288 + BWI76_RS25045 0.42 -1.9
5,213,291 - BWI76_RS25045 0.43 -0.5
5,213,298 + BWI76_RS25045 0.43 -0.5
5,213,306 + +0.4
5,213,306 - BWI76_RS25045 0.44 +1.0
5,213,306 - BWI76_RS25045 0.44 -2.6
5,213,314 - BWI76_RS25045 0.45 +0.7
5,213,314 - BWI76_RS25045 0.45 -1.2
5,213,372 - BWI76_RS25045 0.51 -1.1
5,213,374 + BWI76_RS25045 0.51 +1.4
5,213,459 + BWI76_RS25045 0.60 +1.1
5,213,459 + BWI76_RS25045 0.60 -0.9
5,213,467 - +0.1
5,213,467 - BWI76_RS25045 0.61 +1.1
5,213,467 - BWI76_RS25045 0.61 -1.1
5,213,467 - BWI76_RS25045 0.61 -0.8
5,213,467 - BWI76_RS25045 0.61 -0.5
5,213,503 + BWI76_RS25045 0.65 +1.1
5,213,523 + BWI76_RS25045 0.67 -0.2
5,213,536 + BWI76_RS25045 0.69 +0.2
5,213,548 + BWI76_RS25045 0.70 +0.9
5,213,604 - BWI76_RS25045 0.76 +1.1
5,213,630 + BWI76_RS25045 0.79 +1.1
5,213,638 - +0.1
5,213,675 + BWI76_RS25045 0.83 +0.6
5,213,675 + BWI76_RS25045 0.83 -1.2
5,213,717 + BWI76_RS25045 0.88 +2.6
5,213,774 + -2.8
5,213,777 + +0.4
5,213,777 + +0.1
5,213,777 + +0.1
5,213,782 - -0.6
5,213,785 - -3.0
5,213,810 + +1.8

Or see this region's nucleotide sequence