Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21070 and BWI76_RS21075 are separated by 10 nucleotides BWI76_RS21075 and BWI76_RS21080 are separated by 34 nucleotides BWI76_RS21080 and BWI76_RS21085 are separated by 91 nucleotides
BWI76_RS21070: BWI76_RS21070 - outer membrane protein assembly factor BamB, at 4,408,039 to 4,409,217
_RS21070
BWI76_RS21075: BWI76_RS21075 - membrane protein, at 4,409,228 to 4,409,848
_RS21075
BWI76_RS21080: BWI76_RS21080 - histidine--tRNA ligase, at 4,409,883 to 4,411,157
_RS21080
BWI76_RS21085: BWI76_RS21085 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin), at 4,411,249 to 4,412,370
_RS21085
Position (kb) 4409
4410
4411
4412 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4409.039 kb on - strand, within BWI76_RS21070 at 4409.392 kb on - strand, within BWI76_RS21075 at 4409.438 kb on - strand, within BWI76_RS21075 at 4409.713 kb on - strand, within BWI76_RS21075 at 4409.894 kb on - strand at 4411.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 4,409,039 - BWI76_RS21070 0.85 -1.9 4,409,392 - BWI76_RS21075 0.26 +0.1 4,409,438 - BWI76_RS21075 0.34 -2.8 4,409,713 - BWI76_RS21075 0.78 -0.9 4,409,894 - -2.3 4,411,217 - -1.9
Or see this region's nucleotide sequence