Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14030

Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14025 and BWI76_RS14030 are separated by 24 nucleotidesBWI76_RS14030 and BWI76_RS14035 overlap by 4 nucleotidesBWI76_RS14035 and BWI76_RS14040 overlap by 4 nucleotides BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475 _RS14025 BWI76_RS14030: BWI76_RS14030 - ABC transporter permease, at 2,932,500 to 2,933,240 _RS14030 BWI76_RS14035: BWI76_RS14035 - ABC transporter ATPase, at 2,933,237 to 2,933,965 _RS14035 BWI76_RS14040: BWI76_RS14040 - thiaminase, at 2,933,962 to 2,934,657 _RS14040 Position (kb) 2932 2933 2934Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2931.533 kb on + strandat 2931.538 kb on + strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.546 kb on - strandat 2931.588 kb on - strandat 2931.589 kb on + strandat 2931.600 kb on + strandat 2931.601 kb on + strandat 2931.619 kb on - strandat 2931.620 kb on - strandat 2931.639 kb on - strand, within BWI76_RS14025at 2931.665 kb on + strand, within BWI76_RS14025at 2931.672 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.703 kb on + strand, within BWI76_RS14025at 2931.711 kb on - strand, within BWI76_RS14025at 2931.734 kb on + strand, within BWI76_RS14025at 2931.772 kb on - strand, within BWI76_RS14025at 2931.898 kb on + strand, within BWI76_RS14025at 2932.013 kb on - strand, within BWI76_RS14025at 2932.197 kb on + strand, within BWI76_RS14025at 2932.483 kb on - strandat 2932.527 kb on + strandat 2932.563 kb on + strandat 2932.789 kb on - strand, within BWI76_RS14030at 2932.819 kb on + strand, within BWI76_RS14030at 2933.013 kb on - strand, within BWI76_RS14030at 2933.034 kb on - strand, within BWI76_RS14030at 2933.228 kb on + strandat 2933.236 kb on - strandat 2933.485 kb on - strand, within BWI76_RS14035at 2933.485 kb on - strand, within BWI76_RS14035at 2933.616 kb on - strand, within BWI76_RS14035at 2933.701 kb on + strand, within BWI76_RS14035at 2933.701 kb on + strand, within BWI76_RS14035at 2933.937 kb on - strandat 2933.994 kb on - strandat 2934.013 kb on - strandat 2934.039 kb on - strand, within BWI76_RS14040at 2934.039 kb on - strand, within BWI76_RS14040

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
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2,931,533 + +1.0
2,931,538 + -0.2
2,931,546 - -0.3
2,931,546 - -3.5
2,931,546 - -0.5
2,931,546 - -1.3
2,931,588 - +0.4
2,931,589 + -1.5
2,931,600 + +0.4
2,931,601 + -2.6
2,931,619 - -0.5
2,931,620 - +0.3
2,931,639 - BWI76_RS14025 0.11 +0.3
2,931,665 + BWI76_RS14025 0.13 +0.6
2,931,672 + BWI76_RS14025 0.14 -3.5
2,931,703 + BWI76_RS14025 0.17 -0.5
2,931,703 + BWI76_RS14025 0.17 +0.8
2,931,711 - BWI76_RS14025 0.18 -0.4
2,931,734 + BWI76_RS14025 0.21 -2.1
2,931,772 - BWI76_RS14025 0.25 -0.2
2,931,898 + BWI76_RS14025 0.38 -0.5
2,932,013 - BWI76_RS14025 0.51 +0.3
2,932,197 + BWI76_RS14025 0.70 +0.5
2,932,483 - +0.2
2,932,527 + -0.5
2,932,563 + +0.1
2,932,789 - BWI76_RS14030 0.39 -2.6
2,932,819 + BWI76_RS14030 0.43 -0.2
2,933,013 - BWI76_RS14030 0.69 -0.5
2,933,034 - BWI76_RS14030 0.72 -0.5
2,933,228 + -2.3
2,933,236 - +0.5
2,933,485 - BWI76_RS14035 0.34 -0.9
2,933,485 - BWI76_RS14035 0.34 +1.7
2,933,616 - BWI76_RS14035 0.52 +0.9
2,933,701 + BWI76_RS14035 0.64 -3.9
2,933,701 + BWI76_RS14035 0.64 -0.2
2,933,937 - +1.3
2,933,994 - -0.8
2,934,013 - -2.6
2,934,039 - BWI76_RS14040 0.11 -1.5
2,934,039 - BWI76_RS14040 0.11 +0.4

Or see this region's nucleotide sequence