Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12515 and BWI76_RS12520 are separated by 257 nucleotides BWI76_RS12520 and BWI76_RS12525 are separated by 176 nucleotides BWI76_RS12525 and BWI76_RS12530 are separated by 57 nucleotides
BWI76_RS12515: BWI76_RS12515 - protease, at 2,614,296 to 2,614,859
_RS12515
BWI76_RS12520: BWI76_RS12520 - hypothetical protein, at 2,615,117 to 2,615,320
_RS12520
BWI76_RS12525: BWI76_RS12525 - beta-1,4-xylosidase, at 2,615,497 to 2,617,176
_RS12525
BWI76_RS12530: BWI76_RS12530 - sugar (glycoside-Pentoside-Hexuronide) transporter, at 2,617,234 to 2,618,688
_RS12530
Position (kb)
2615
2616
2617
2618 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2614.625 kb on - strand, within BWI76_RS12515 at 2614.653 kb on + strand, within BWI76_RS12515 at 2614.889 kb on + strand at 2614.901 kb on - strand at 2614.901 kb on - strand at 2614.963 kb on + strand at 2615.139 kb on + strand, within BWI76_RS12520 at 2615.227 kb on + strand, within BWI76_RS12520 at 2615.232 kb on + strand, within BWI76_RS12520 at 2615.232 kb on - strand, within BWI76_RS12520 at 2615.304 kb on + strand at 2615.312 kb on - strand at 2615.412 kb on - strand at 2615.485 kb on + strand at 2615.493 kb on - strand at 2615.604 kb on - strand at 2615.619 kb on - strand at 2615.630 kb on - strand at 2615.680 kb on - strand, within BWI76_RS12525 at 2615.753 kb on - strand, within BWI76_RS12525 at 2615.773 kb on - strand, within BWI76_RS12525 at 2615.811 kb on - strand, within BWI76_RS12525 at 2615.862 kb on + strand, within BWI76_RS12525 at 2615.865 kb on - strand, within BWI76_RS12525 at 2615.963 kb on + strand, within BWI76_RS12525 at 2615.972 kb on - strand, within BWI76_RS12525 at 2616.039 kb on + strand, within BWI76_RS12525 at 2616.090 kb on + strand, within BWI76_RS12525 at 2616.130 kb on + strand, within BWI76_RS12525 at 2616.166 kb on - strand, within BWI76_RS12525 at 2616.177 kb on + strand, within BWI76_RS12525 at 2616.177 kb on + strand, within BWI76_RS12525 at 2616.259 kb on - strand, within BWI76_RS12525 at 2616.323 kb on - strand, within BWI76_RS12525 at 2616.343 kb on - strand, within BWI76_RS12525 at 2616.353 kb on - strand, within BWI76_RS12525 at 2616.384 kb on - strand, within BWI76_RS12525 at 2616.475 kb on - strand, within BWI76_RS12525 at 2616.514 kb on + strand, within BWI76_RS12525 at 2616.523 kb on - strand, within BWI76_RS12525 at 2616.619 kb on - strand, within BWI76_RS12525 at 2616.702 kb on - strand, within BWI76_RS12525 at 2616.744 kb on - strand, within BWI76_RS12525 at 2616.776 kb on - strand, within BWI76_RS12525 at 2616.793 kb on + strand, within BWI76_RS12525 at 2616.801 kb on - strand, within BWI76_RS12525 at 2616.806 kb on + strand, within BWI76_RS12525 at 2616.814 kb on - strand, within BWI76_RS12525 at 2616.907 kb on + strand, within BWI76_RS12525 at 2616.907 kb on + strand, within BWI76_RS12525 at 2616.915 kb on - strand, within BWI76_RS12525 at 2616.916 kb on - strand, within BWI76_RS12525 at 2616.933 kb on + strand, within BWI76_RS12525 at 2616.961 kb on - strand, within BWI76_RS12525 at 2617.067 kb on + strand at 2617.075 kb on - strand at 2617.187 kb on - strand at 2617.187 kb on - strand at 2617.239 kb on + strand at 2617.261 kb on + strand at 2617.300 kb on - strand at 2617.325 kb on - strand at 2617.337 kb on + strand at 2617.547 kb on - strand, within BWI76_RS12530 at 2617.565 kb on - strand, within BWI76_RS12530 at 2617.566 kb on - strand, within BWI76_RS12530 at 2617.590 kb on + strand at 2617.590 kb on + strand, within BWI76_RS12530 at 2617.598 kb on - strand, within BWI76_RS12530 at 2617.598 kb on - strand, within BWI76_RS12530 at 2617.631 kb on - strand, within BWI76_RS12530 at 2617.638 kb on - strand, within BWI76_RS12530 at 2617.649 kb on - strand, within BWI76_RS12530 at 2617.787 kb on + strand, within BWI76_RS12530 at 2617.787 kb on + strand, within BWI76_RS12530 at 2617.800 kb on - strand, within BWI76_RS12530 at 2617.815 kb on - strand, within BWI76_RS12530 at 2617.831 kb on + strand, within BWI76_RS12530 at 2617.839 kb on - strand at 2617.889 kb on + strand, within BWI76_RS12530 at 2617.937 kb on + strand, within BWI76_RS12530 at 2617.956 kb on + strand, within BWI76_RS12530 at 2617.964 kb on - strand, within BWI76_RS12530 at 2617.964 kb on - strand, within BWI76_RS12530 at 2617.997 kb on - strand, within BWI76_RS12530 at 2618.015 kb on + strand, within BWI76_RS12530 at 2618.118 kb on + strand, within BWI76_RS12530 at 2618.126 kb on - strand, within BWI76_RS12530 at 2618.126 kb on - strand, within BWI76_RS12530 at 2618.169 kb on + strand, within BWI76_RS12530 at 2618.169 kb on + strand, within BWI76_RS12530 at 2618.169 kb on + strand, within BWI76_RS12530 at 2618.169 kb on + strand, within BWI76_RS12530
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,614,625 - BWI76_RS12515 0.58 -0.6 2,614,653 + BWI76_RS12515 0.63 -0.5 2,614,889 + -0.1 2,614,901 - +0.6 2,614,901 - -1.6 2,614,963 + +0.0 2,615,139 + BWI76_RS12520 0.11 +0.7 2,615,227 + BWI76_RS12520 0.54 +0.8 2,615,232 + BWI76_RS12520 0.56 +1.0 2,615,232 - BWI76_RS12520 0.56 -1.3 2,615,304 + -0.4 2,615,312 - +0.4 2,615,412 - -2.0 2,615,485 + +0.0 2,615,493 - +0.2 2,615,604 - +0.3 2,615,619 - -3.2 2,615,630 - -0.4 2,615,680 - BWI76_RS12525 0.11 -0.3 2,615,753 - BWI76_RS12525 0.15 +0.5 2,615,773 - BWI76_RS12525 0.16 -0.6 2,615,811 - BWI76_RS12525 0.19 +0.2 2,615,862 + BWI76_RS12525 0.22 -1.0 2,615,865 - BWI76_RS12525 0.22 +0.0 2,615,963 + BWI76_RS12525 0.28 -1.3 2,615,972 - BWI76_RS12525 0.28 -0.1 2,616,039 + BWI76_RS12525 0.32 +0.3 2,616,090 + BWI76_RS12525 0.35 -1.6 2,616,130 + BWI76_RS12525 0.38 -1.8 2,616,166 - BWI76_RS12525 0.40 -0.7 2,616,177 + BWI76_RS12525 0.40 -0.6 2,616,177 + BWI76_RS12525 0.40 +1.7 2,616,259 - BWI76_RS12525 0.45 +0.4 2,616,323 - BWI76_RS12525 0.49 -1.6 2,616,343 - BWI76_RS12525 0.50 +1.0 2,616,353 - BWI76_RS12525 0.51 +1.0 2,616,384 - BWI76_RS12525 0.53 -1.0 2,616,475 - BWI76_RS12525 0.58 +1.3 2,616,514 + BWI76_RS12525 0.61 -1.0 2,616,523 - BWI76_RS12525 0.61 -3.3 2,616,619 - BWI76_RS12525 0.67 -0.8 2,616,702 - BWI76_RS12525 0.72 +0.5 2,616,744 - BWI76_RS12525 0.74 -2.1 2,616,776 - BWI76_RS12525 0.76 -0.1 2,616,793 + BWI76_RS12525 0.77 -0.5 2,616,801 - BWI76_RS12525 0.78 -1.7 2,616,806 + BWI76_RS12525 0.78 +1.3 2,616,814 - BWI76_RS12525 0.78 -0.2 2,616,907 + BWI76_RS12525 0.84 -1.7 2,616,907 + BWI76_RS12525 0.84 -0.1 2,616,915 - BWI76_RS12525 0.84 -2.7 2,616,916 - BWI76_RS12525 0.84 -2.1 2,616,933 + BWI76_RS12525 0.85 -0.6 2,616,961 - BWI76_RS12525 0.87 +0.2 2,617,067 + +2.6 2,617,075 - +1.0 2,617,187 - -1.3 2,617,187 - +1.1 2,617,239 + +1.4 2,617,261 + -0.6 2,617,300 - +0.3 2,617,325 - -0.3 2,617,337 + -3.3 2,617,547 - BWI76_RS12530 0.22 -0.4 2,617,565 - BWI76_RS12530 0.23 +0.0 2,617,566 - BWI76_RS12530 0.23 +1.0 2,617,590 + +0.0 2,617,590 + BWI76_RS12530 0.24 -1.0 2,617,598 - BWI76_RS12530 0.25 -0.2 2,617,598 - BWI76_RS12530 0.25 -1.4 2,617,631 - BWI76_RS12530 0.27 -1.6 2,617,638 - BWI76_RS12530 0.28 +1.0 2,617,649 - BWI76_RS12530 0.29 -0.4 2,617,787 + BWI76_RS12530 0.38 -0.0 2,617,787 + BWI76_RS12530 0.38 -0.9 2,617,800 - BWI76_RS12530 0.39 -0.1 2,617,815 - BWI76_RS12530 0.40 +1.1 2,617,831 + BWI76_RS12530 0.41 -0.0 2,617,839 - +1.0 2,617,889 + BWI76_RS12530 0.45 -1.1 2,617,937 + BWI76_RS12530 0.48 -1.3 2,617,956 + BWI76_RS12530 0.50 +0.4 2,617,964 - BWI76_RS12530 0.50 -1.3 2,617,964 - BWI76_RS12530 0.50 -0.8 2,617,997 - BWI76_RS12530 0.52 +0.2 2,618,015 + BWI76_RS12530 0.54 -1.8 2,618,118 + BWI76_RS12530 0.61 -0.4 2,618,126 - BWI76_RS12530 0.61 -3.7 2,618,126 - BWI76_RS12530 0.61 +0.2 2,618,169 + BWI76_RS12530 0.64 -2.4 2,618,169 + BWI76_RS12530 0.64 -0.5 2,618,169 + BWI76_RS12530 0.64 +0.4 2,618,169 + BWI76_RS12530 0.64 +0.6
Or see this region's nucleotide sequence