Experiment: RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11765 and BWI76_RS11770 are separated by 134 nucleotides BWI76_RS11770 and BWI76_RS11775 are separated by 91 nucleotides BWI76_RS11775 and BWI76_RS11780 are separated by 148 nucleotides BWI76_RS11780 and BWI76_RS11785 are separated by 130 nucleotides
BWI76_RS11765: BWI76_RS11765 - bifunctional transporter/kinase, at 2,475,327 to 2,476,718
_RS11765
BWI76_RS11770: BWI76_RS11770 - hypothetical protein, at 2,476,853 to 2,477,443
_RS11770
BWI76_RS11775: BWI76_RS11775 - 2-deoxy-D-gluconate 3-dehydrogenase, at 2,477,535 to 2,478,296
_RS11775
BWI76_RS11780: BWI76_RS11780 - 2-deoxyglucose-6-phosphatase, at 2,478,445 to 2,479,113
_RS11780
BWI76_RS11785: BWI76_RS11785 - hypothetical protein, at 2,479,244 to 2,479,780
_RS11785
Position (kb)
2477
2478
2479 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2476.638 kb on + strand at 2476.643 kb on - strand at 2476.937 kb on + strand, within BWI76_RS11770 at 2476.976 kb on - strand, within BWI76_RS11770 at 2477.275 kb on - strand, within BWI76_RS11770 at 2477.451 kb on + strand at 2477.575 kb on - strand at 2477.628 kb on - strand, within BWI76_RS11775 at 2477.829 kb on - strand, within BWI76_RS11775 at 2478.275 kb on + strand at 2478.283 kb on - strand at 2478.340 kb on + strand at 2478.613 kb on + strand, within BWI76_RS11780 at 2478.926 kb on + strand, within BWI76_RS11780 at 2479.249 kb on - strand at 2479.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Sinorhizobium meliloti 1021 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 1 x remove 2,476,638 + +0.9 2,476,643 - -1.6 2,476,937 + BWI76_RS11770 0.14 -0.1 2,476,976 - BWI76_RS11770 0.21 +0.1 2,477,275 - BWI76_RS11770 0.71 -0.7 2,477,451 + -1.3 2,477,575 - +0.2 2,477,628 - BWI76_RS11775 0.12 -0.9 2,477,829 - BWI76_RS11775 0.39 -1.3 2,478,275 + -0.6 2,478,283 - +0.0 2,478,340 + -3.6 2,478,613 + BWI76_RS11780 0.25 +0.4 2,478,926 + BWI76_RS11780 0.72 -2.3 2,479,249 - +1.8 2,479,249 - +0.4
Or see this region's nucleotide sequence